FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTGAGGAACTTGTAATTTGTGGTAGAAGCAGAATTCCTGATGACAAC52.793296089385475No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG42.2346368715083798Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG42.2346368715083798Clontech Universal Primer Mix Long (96% over 26bp)
TATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG31.675977653631285Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31.675977653631285Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACGACGCTCTTCCGATCTCAGACCATGAGGCAGTCCCCGCATCATGGT31.675977653631285No Hit
GTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACA31.675977653631285Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGCTGAGCCATGGAATCTGTTTTCTATCTCCCAGTTTAAAGTCCTCAAG31.675977653631285No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG21.1173184357541899No Hit
GTACATGGGTGATAGCCACAGTCTACACTCTTTCCCTACACGGAGCTGCC21.1173184357541899Illumina Single End PCR Primer 1 (95% over 21bp)
GCGCACGCCCGAGGAATATGCGGCAGGCCACCTGCCCGGCTTCGCCAGCG21.1173184357541899No Hit
GTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCAGAGTACAT21.1173184357541899Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCAGCCCCGTGTCTGGGAGCCTGTGCCTCTGGAGGAGGTCCAATTGGT21.1173184357541899No Hit
AGACAACAATAAATATTAAGTAACTGCTCATGAATTTTGTGGGTAAAGAG21.1173184357541899No Hit
ATGCCGACGTTTACGTGATGGCCGACGGCGACGCCACCTACGACGCGGGC21.1173184357541899No Hit
GTCTGCATTGCTGCCAGCATTATCTTTCCAAGCAAATCTGCTTCGAATCC21.1173184357541899No Hit
GGTGAGCAGTTCTTTCAAAGCCTCAGCTAAACAAAGGACATTCTTCCCTT21.1173184357541899No Hit
AGATTAGATCAATATCTCTCAAATAGTCTTTCACAGTCAGATACTTGTGT21.1173184357541899No Hit
CTCACCCATCCATCCAACCTTCCAACCATTTATCCACCCATCCAACTATT21.1173184357541899No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA21.1173184357541899Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCTCAGATGAAACCATACTTGAGATACTCAGAACATACTACACTATAC21.1173184357541899No Hit
CCTGTAATCCCAGCACATTGGGAAGCGGCTCAAGCGATCTTCCCATCACA21.1173184357541899No Hit
ATCTGATGGCCCCTTTCTATGTGAGGCAGGATAGCATTACAGACAGGAAG21.1173184357541899No Hit
GAGTGTAATCTCGGTGGTCGCCGTATCACCCATGTACTCCTGTCTCTTAT21.1173184357541899Illumina Single End PCR Primer 1 (95% over 22bp)
CTTTCTGGTAGCTCTTGAACACGAACGGGCCAGTGCCGATCGGCTTCTGG10.5586592178770949No Hit
AACATTCACGGCCATCTAAGTCCCAACCTGCCGATGAAGCAACAAAGCTC10.5586592178770949No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATTA10.5586592178770949Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAGTAATCTGCGTTGATACCACTGCT10.5586592178770949Clontech SMART CDS Primer II A (96% over 25bp)
GTTGAGATTCATCCTGAAATCAGAGAGACTTTCAGAGGATGATAAAAATG10.5586592178770949No Hit
GGGAGATGGGAAGTGGCAGGAAAGCAAAGTGTTGGAGGAACCAAAGAGAA10.5586592178770949No Hit
CTTTTAGAGTAGATCTGTGGAAAGCACCCATGTTTACCAATCCCTTCTAT10.5586592178770949No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTACCAACGCAGAGTACATGGGAA10.5586592178770949Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGCT10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAAAGCAGAGTACATTGGAAATAAAATCAAAC10.5586592178770949Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTTATTTACAGTCTTTCTGGAACTTTTCTTTTATACATCTAAACCTCAAT10.5586592178770949No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA10.5586592178770949No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGG10.5586592178770949No Hit
GTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCCGC10.5586592178770949No Hit
GACGTCGGTAGGGAAGATGTAGTGTGGTTCTCCCATGTACTCTGCGTTGA10.5586592178770949No Hit
CTGCTTACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10.5586592178770949Clontech SMART CDS Primer II A (100% over 26bp)
CAACCTCGACTTCCCGCCCGGCACCCACGTCCGCGTGCATCCGCTGCTGC10.5586592178770949No Hit
GGCCGGTTTCCCGGCCCCCTCTTCAATCCTCCTGTCTCTTATACACATCT10.5586592178770949No Hit
GTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGGC10.5586592178770949No Hit
GCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTC10.5586592178770949No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGTCCACCGAGAGCTACA10.5586592178770949Illumina Single End PCR Primer 1 (95% over 22bp)
CATGTAAGCAGTGGTATCAACGCAGAGTGCTTACATGTACTCTGCGTTGA10.5586592178770949Clontech SMART CDS Primer II A (96% over 26bp)
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC10.5586592178770949No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTT10.5586592178770949Illumina Single End PCR Primer 1 (100% over 38bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
GATCTACATCGGCAGCCAGGGCATCGTGCAGGGCACCTACGAGACCTTCG10.5586592178770949No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC10.5586592178770949No Hit
CGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGGTGTAA10.5586592178770949No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA10.5586592178770949Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGA10.5586592178770949Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT10.5586592178770949No Hit
GGTGACGGGCAGCTCAGGCCACAGGGTCACTTGATGTCCCATGAACTAGG10.5586592178770949No Hit
CTTCCATGGCATTCAGGACCCCCGACAGCTGAAGCCTCTCTTCATATAGC10.5586592178770949No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.5586592178770949Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAGG10.5586592178770949No Hit
GTGTTGACTTGCAAGATTTTGCGTTTATTCAGGCAAAAACTGGTCAAAAT10.5586592178770949No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGTGCCATGTACTCTGCGTTGATACC10.5586592178770949Illumina Single End PCR Primer 1 (96% over 28bp)
GAGCTTTTGTTACTGTCTCAGAGGTCTCTTGTGCTTTCATGTTTACCCCC10.5586592178770949No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.5586592178770949Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCACCATGGCGTCGCGCGGGCTCGACCAAGCCGTCGGCACCAACACCA10.5586592178770949No Hit
GATGAGATTAGGGTGGGGACACAAAGCCAAACCATATCACAACTCAAATT10.5586592178770949No Hit
CATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG10.5586592178770949No Hit
GTGCACCACCACGTCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGATT10.5586592178770949No Hit
GCATGCGGTTGTTCGGAATGTCCGACCACACCAGCGTGCGGTGCGCCGCG10.5586592178770949No Hit
ACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATACTGTCT10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
GAGTGTAGATCTCGGTGGTCGCCGTATCATCCCATGTACTCTGCGTTGAT10.5586592178770949Illumina Single End PCR Primer 1 (96% over 30bp)
GTTAACCTCAAATGTAAATGGGCTAAATGCTCCAATTAAAAGACACAGAC10.5586592178770949No Hit
GTGCATGGGGAAGCAGTGGTATCAACGCAGAGGTACTTTTTTTTTTTTTT10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACC10.5586592178770949Clontech Universal Primer Mix Long (95% over 21bp)
CCCTAAGCCCCCACTGCCTTCCTTAGTGATAGCAGTCATAAATATCAGGG10.5586592178770949No Hit
GGTCTGTACTGTGTGTAGTGCTGTTTACATAGATGAATTTAGGTTGTAAT10.5586592178770949No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA10.5586592178770949No Hit
GTGTAGGAAAGATGTAATCTCGGTGTCGCGATACCCATGTACTCTGCGTT10.5586592178770949No Hit
ACGCAGAGTACATGGGAATGATACGGCGACCACCGGTGTAGATCTCGGTG10.5586592178770949No Hit
CCCGGGTGTAGGCCTGTCGTCGATGCGGGAGCGCGCCGAGGAGCTCGGCG10.5586592178770949No Hit
CTCTTTCCCTACACGACGCTCTTCCTATCTCTGTCCCAAGTTCTGCAGGT10.5586592178770949Illumina Single End PCR Primer 1 (96% over 30bp)
CCCAGGACCAGACCGATTCACAGCCGAATTCTACCAGAGGTACAAGGAGG10.5586592178770949No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT10.5586592178770949Clontech SMART CDS Primer II A (100% over 25bp)
ACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGGAAGG10.5586592178770949No Hit
GTACATGGAGAGATCTACACTCTTTCCCTACAGCAACTCCAGTCCTTGCA10.5586592178770949Illumina Single End PCR Primer 1 (95% over 23bp)
GGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACC10.5586592178770949No Hit
CCGGCAAGCCGCCCGCCCGCCGCAGCAGGTCGATCGCCGGGTCGAGCTGC10.5586592178770949No Hit
CTTCAAAGGTCAAGGATAATCCTCCCTCTGGTCTTAGTTTGCTTTAAGAA10.5586592178770949No Hit
GCCTCGAAAGAGTCCTGTATTGTTATTTTTCGACACTACCTCCCCGGGTC10.5586592178770949No Hit
AATTTCCTGAGCAACAGGGGTGCTAGGTGTTTGGTACATCTCTTTTTTTT10.5586592178770949No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGAGTCTGTCT10.5586592178770949Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.5586592178770949Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGTAAAAAAAAAAAAAAAAAAAAAAATTAC10.5586592178770949No Hit
ATTTTTTTTTCAGTCTTTTTTTCTTTCTTCTTCAGATTAGACAAATGTCA10.5586592178770949No Hit
ACCGAGATCTACACTCTTTCCCTACACGACGCTGATCGGAAGAGCGTCGT10.5586592178770949Illumina Single End PCR Primer 1 (96% over 33bp)
ATTCATGGTGGCTGGCAGTAATGTACGAAATACATAGCGGTTGTTGATGG10.5586592178770949No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTG10.5586592178770949Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTGAGTGGGAAAGATTTTTCCCCTAATAAAAAGAGGTGCACAGGAGGA10.5586592178770949No Hit
ACCCCGAGGCGGCCAACCGCGTGATGGAAACCGAGCACCGCGTCAATGCG10.5586592178770949No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
CTCAGACACAGATGTCTTAAATATAAAAAATTAGCCAGGTATGGTGGCAT10.5586592178770949No Hit
GTACTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCAAGAACT10.5586592178770949Clontech Universal Primer Mix Long (95% over 21bp)
TTGTCCATTACCGTAAAACTTAATGGTGGACAAGTTAGCTGTGGTTGATT10.5586592178770949No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
ATGCAACACCATTCTCGGGCAACACAAGGAGGCCCTGGGCTAGAGACTGT10.5586592178770949No Hit
ATCCTGCCCCCATCATTTCCGCTGACATCATTTCCCTCTGTCTCTTATAC10.5586592178770949No Hit
TGCTTCCCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
GATATATTGGGAAGGAAACTCTTGAAGATAAGAATTTTTTCTTTTTTTTT10.5586592178770949No Hit
GGTTGTGATGTTTACGTTCCATTTTCCAGGCTGAGAATGAATTAGGTTAC10.5586592178770949No Hit
AACAACAGAAAGAACATGCTGAATTGATTGAAGATTATCGGATCAAACAG10.5586592178770949No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAATTACCT10.5586592178770949Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTCA10.5586592178770949Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCGAGGAGTTCCCGTACGCCATCCGCCAGGTCTCCGAGGCACTCGGCTC10.5586592178770949No Hit
GTTTTGTAATAAAGTGCTGAATATTTCAAGTAACTTACTGAATACTGTAC10.5586592178770949No Hit
ATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGAT10.5586592178770949No Hit
GTATCAACGCAGAGTACATGGGAACCACGAGATACATCTTTCCCCTGTCT10.5586592178770949No Hit
AATCAACCCACAGAAAATGAGAAAAACTGTAAAAAAAACACATAACATAG10.5586592178770949No Hit
GTGTGGTGGCTTGTGCCTGTAGTCCCAGCTACTCAAGAGGCTGAGGTGAG10.5586592178770949No Hit
GCTTTCATCACTTTCTTCCTGCCTTAACTTCTACATCTGTTTAGCCACCA10.5586592178770949No Hit
CAAACACTACTCTAAAACCATCTAAATGTCTAACGGTTCCTCACCCAATT10.5586592178770949No Hit
GGATACAGAACAGTTCTACCACCCAAAAAGGCCTTCTTGCTGTCCTTTCA10.5586592178770949No Hit
CAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAAAT10.5586592178770949No Hit
ACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGTGCC10.5586592178770949Clontech Universal Primer Mix Long (100% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5586592178770949No Hit
CTCTTTTGGCTGATTGCAATTCTGGCCCAACTCAACCCTCTCTTTGGACC10.5586592178770949No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.5586592178770949No Hit
ACGCTCTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGCGTCGTGTA10.5586592178770949No Hit
TATTAAACGTTGGTACGAAGAAAATAAAAAATATATGCCGCGCCTACCTC10.5586592178770949No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTGGCATAGGGCCAGACCCTAGGCCTGAAAAGGATGCAAAGCCTGCT10.5586592178770949No Hit
CCATTTGATGATTCCATTCAATTCAAGTCAATGATGATTCCATTCGATTC10.5586592178770949No Hit
ATATTATAGATTTTAGTATTCTCTAATTCAGGTGCCAGCAAAGTTTGGCC10.5586592178770949No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATCGGCGAC10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGACGGCGAACCGAGTCACACTTCCCTACACCTGTC10.5586592178770949No Hit
ATATACACATTTGCATTTCTGTTATTAGCGACTCTAAACACCATTTGAAA10.5586592178770949No Hit
AAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGG10.5586592178770949No Hit
ACCGAGATCTACACTCTTTCCCTACACGACGCTCATCCGATCTCTGTCCC10.5586592178770949Illumina Single End PCR Primer 1 (97% over 43bp)
CTTCTGGGGGTATCAGCCCGTCGGCTACTTCGCCCCGGACCCGCGCTACC10.5586592178770949No Hit
AGATGGACTGGCCCTTGTCGGACAGCACATAGTCCAGCACCTTCTTGCCG10.5586592178770949No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTGGTCCTCGCCGGCGTCGCGGCTGTTGGCGGCGGCGAAGCGCTCCA10.5586592178770949No Hit
GTTCACCCACTAATAGGGAACGTGAGCTGGGATTAGACCGTCGTGAGACA10.5586592178770949No Hit

[FAIL]Adapter Content

Adapter graph