FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG95.027932960893855Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATGTGAAAAGGGACTTTACTTTGAAATTAAATCTGATAAATCACTTTT52.793296089385475No Hit
AAAGGAGACTGACGGAGAGGATTCTTCCTGGATCCCAGCACTTCCTCTGA31.675977653631285No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG31.675977653631285Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG31.675977653631285Clontech Universal Primer Mix Long (96% over 26bp)
CACCCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTTGA31.675977653631285Clontech SMART CDS Primer II A (96% over 25bp)
GAGTACATGGGTGATACGGCGACCACCGAGATTACACTCCTGTCTCTTAT21.1173184357541899Illumina Single End PCR Primer 1 (95% over 21bp)
GCGCGGCCAGTTCGGCCGGGGCGATCTCGTTCACTGCGACGGCAACCGGG21.1173184357541899No Hit
CCCTACACGACGCCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGGG21.1173184357541899No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.1173184357541899Clontech Universal Primer Mix Long (96% over 26bp)
ACCTACAGCTGTGTGGCCACCCATTCCAGCCACGGGCCCCAGGAAAGGGA21.1173184357541899No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.1173184357541899Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAAATTATCAACTGCCATAGGGCTGCCATGTTAGGAGGAAGCCTAAA21.1173184357541899No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC21.1173184357541899No Hit
GAAGGATGGGTGGCAATTGGATGAGGAAAGAATATAGGCTGTTACTGGAG21.1173184357541899No Hit
AGCTTGCTCACCGAGCCTTCGGGCCGCGTGCTGTAGGCCGTGGCTTCCTC21.1173184357541899No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT21.1173184357541899Clontech SMART CDS Primer II A (100% over 26bp)
GCGCCCGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCA21.1173184357541899No Hit
GCCTAAACTCTTTTCGCAAGAAGCACAGCGTGTTTTGAGCACGGCTTGAA21.1173184357541899No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21.1173184357541899Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.1173184357541899Clontech Universal Primer Mix Long (96% over 26bp)
GGATGGATGAGTGGGTGGATGGATGGGTAGATGGGTGATAAATGGATAGG21.1173184357541899No Hit
GAAAATGAGGTGGTCTGTGTTCCTGTACTATAAGGTCAGTGGTAGAATTT21.1173184357541899No Hit
GGGCTGCTACCTATGAAGCCCTACTATACTCAAGTCTACCAGAAGATTTG21.1173184357541899No Hit
ATCCACTTGCTTGTTCCTCATGAAAGCCAAGTGAGAGGGGGCTTTGTCAC21.1173184357541899No Hit
GGCTGCCCATGCTAAAGAGATGGAAGCCTTGAGGGCTAAACTGATGAAAG21.1173184357541899No Hit
ACCGAGATCTACATTTTCCCTACACGACGTCTTGCCTCAGATTCTGTCGG10.5586592178770949No Hit
GGCAAGGAAGAGCGTCGTGTAGGGAAAGATAGATCTCGGTGGTCGCCGTA10.5586592178770949Illumina Single End PCR Primer 1 (95% over 24bp)
AGGGAAATGATGTCAGCGGAAATGATGGGGGCAGGATCTGTCTCTTATAC10.5586592178770949No Hit
GCCCTCGGCGGTGTGGTTGTAGACCACGTCGAGCCAGGTCTCGATCCCGG10.5586592178770949No Hit
ATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGT10.5586592178770949No Hit
GTGTAGGGAAGTGTGACTCGGTTCGCCGTCCCATGTACTCTGCGTCTGTC10.5586592178770949No Hit
AACAGGCATTGGGTTAGATTTGGCTCATAGGCCAAACTTTGCTGGCACCT10.5586592178770949No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAATTAACAAGGCGTTTTAAACAAA10.5586592178770949No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTCTGCGTTGATACCA10.5586592178770949Clontech SMART CDS Primer II A (100% over 21bp)
GAGGTCACAGTGATGGCAGCCTGGGGCCAGACAAGGGATGCGTGTCTTGT10.5586592178770949No Hit
CACCTGGATGGCAGCAGGGGCGCCGGGGTCCTCTCGACGCCAGAGAGAAA10.5586592178770949No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTAAGTTTATAACAAAGAATACCAA10.5586592178770949No Hit
GTATCAACGCAGAGTACCAGCAGCGGGAGCAGGGGTTTCCTCCTGTGCAC10.5586592178770949No Hit
AGGCTGGACTGCAATGGTGTAATCTCAGCTCACTGCAACCTCCACCTCCC10.5586592178770949No Hit
TGAAAAACGAGCGTCGTGTAGAGAAAGAGAGATCTCTGCGCCCATGTACT10.5586592178770949No Hit
CGACTTGACCATGCGCGCGATCTTGTTGGCCTCGTCGCCCACGCGCTCGA10.5586592178770949No Hit
ACTCTAGACTGAATCAGGCAGGGCTGCTACATGATGGTACAGCCAAGTTT10.5586592178770949No Hit
CTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGC10.5586592178770949No Hit
GGCCTGCTCGTCGAGGTAGCGCGTCGCGAGCCGCATGGCTATCCGGCTCT10.5586592178770949No Hit
GGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC10.5586592178770949No Hit
GCCATACATCAATAAGACTGCATCTTTCCAGAAAAGGCATGTTTTTCCAA10.5586592178770949No Hit
GGAGGGAGTTATGGTAAGTAACTTCCAAAAATCTGTGGGTAAACTCCCTT10.5586592178770949No Hit
GAGGATTGAAGAGGGGGCCGGGAAACCGGCCCTGTCTCTTATACACATCT10.5586592178770949No Hit
TATTTGTTCTCTGCTTGCAGTTTCTGGCACAGAGCTCCTAAAACCCTTGG10.5586592178770949No Hit
GGGAAAGATGTATCTCGTGGTTCCCATGTACTCTGCGTTGATACCTGTCT10.5586592178770949No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAGTACC10.5586592178770949Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGTGATACGGCGACCACCGAGATCTACACTCTTTCCATACACG10.5586592178770949Illumina Single End PCR Primer 1 (97% over 41bp)
ATCAACGCAGAGTACATGGGATGATCGGCGACCACCGAGATCTACACTCT10.5586592178770949Illumina Single End PCR Primer 1 (96% over 26bp)
GTACATGGGTGATACGGCGCCACCGAGATCTACACTCTTTCCCTACACGA10.5586592178770949Illumina Single End PCR Primer 1 (100% over 31bp)
CATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCG10.5586592178770949No Hit
GCATTACAATTCCTGTCACAGACTACAAGTTAATTTTCTTAACTGTAATA10.5586592178770949No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5586592178770949Clontech SMART CDS Primer II A (100% over 22bp)
AAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGG10.5586592178770949No Hit
GATTCGATTTTAATCATCATCGAATGGACTTGAATTGAATTATCGAATGG10.5586592178770949No Hit
GGCCTGGCCCTGGTCGAGCGGCAGCCGCACCTCGACCACGGTGCCGCCGC10.5586592178770949No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATGATACGGC10.5586592178770949Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTGGAGATCTTCCTCCATCCCTTTATTTTGAGCCTATGCGTGTCTCTG10.5586592178770949No Hit
GCATATGGCAGAAAAAAAGAATCAGTGAGCTGAAAAAAAGTCAACAGAAA10.5586592178770949No Hit
GTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.5586592178770949Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAAC10.5586592178770949No Hit
CCTCGTATGCGCGCCTGCTGTCGGGCGAGATCGGCATCCTGCTGGACTAC10.5586592178770949No Hit
TCGCGCCGGAGACGGCGACGGAGGCCGAGCGCGAGGGCTGGGCGGCGGGC10.5586592178770949No Hit
GATATGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA10.5586592178770949No Hit
ACTCAGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCT10.5586592178770949Clontech Universal Primer Mix Long (95% over 23bp)
ATTTTCCTGGTATAAAAAGACATTATTCTCAAACAATAACTTTCTAAAAA10.5586592178770949No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10.5586592178770949Clontech Universal Primer Mix Long (96% over 25bp)
GTTGCAGTGAGCTGAGATCACACCATTGCACTCCAGACTGGGCAACAAGA10.5586592178770949No Hit
TATCAACGCAGAGTACATGGGAAGAGTGGTATCAACGCAGAGTACATGGG10.5586592178770949Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GTCATGGGAAGCAGTGGTATCAACGCATAGTACATGGGAAGCAGTGGTAT10.5586592178770949Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATCTCAGTGACATGCTAAGTGAAGGAAGCTAGACTCAAAAGGTGACATAC10.5586592178770949No Hit
CGTTCGAGCCGAGTGCCTCGGAGACCTGGCGGATGGCGTACGGGAACTCC10.5586592178770949No Hit
GAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATAGCGT10.5586592178770949No Hit
GTGTAGGGAAGAGTGTAATCTCGGTGGGACACCCATGTACTCTGCGTTGA10.5586592178770949No Hit
CTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGAGCGTCGTGTAGGG10.5586592178770949Illumina Single End PCR Primer 1 (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.5586592178770949Clontech Universal Primer Mix Long (100% over 23bp)
TCTCTAGCCCAGGGCCTCCTTGTGTTGCCCGAGAATGGTGTTGCATCTGT10.5586592178770949No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTACCAA10.5586592178770949No Hit
CCGTACATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
CGCGTGCCCCGCCGCGCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTC10.5586592178770949No Hit
CTCTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGCGTCGTGTAGGG10.5586592178770949No Hit
TGGCTGGCCTTGGCTCTCCAAGAGCTCTAAATCGCCTGGGAATTAATGCA10.5586592178770949No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGAAG10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTTAT10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
CGTCTCTACTAAAAATACAGGAAAATTAGCTGGGCTTGGTGGCGCATGCC10.5586592178770949No Hit
CCCTTGGCGAAATACAGCGGCACGATTGGGATTTCCTCCCGCGCGATGTA10.5586592178770949No Hit
GATTTACACTCTTTCCCTACACGACGTCTTCCGATCTGTCCCAGGTCTTG10.5586592178770949Illumina Single End PCR Primer 1 (96% over 26bp)
GTACATGGGTATCAACGCAGAGTACATGGGAGAACCACACTACATCTTCC10.5586592178770949No Hit
ACTCTGAGATGATTAGACATGCAGTGTTTACTTGATGACTTTCTGTATTT10.5586592178770949No Hit
TCCCCAAATACTGTGAAATTGGCAATAATCCAGGCAACTCCAAACTTCTG10.5586592178770949No Hit
CCTCCACCCTCCCCCACCATCTGATAGTACCTCATTCCTGCCTAAGGCTT10.5586592178770949No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTG10.5586592178770949Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGATACCACTTCTTCCT10.5586592178770949No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGTCGGCGAC10.5586592178770949Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
CCTATAGGCTCCATACGGCTTCTTTATTCTAGCCTAGCCTAACCTACAAG10.5586592178770949No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT10.5586592178770949Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTAATTGACTCCTAGCCGCAGACATCCTCAATCTAACCTGAATCGGAGG10.5586592178770949No Hit
TCCCTAAGGGTCCTAACCGGGGAGTTATCATTACCATGTTGGTGACCTGT10.5586592178770949No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.5586592178770949No Hit
GATTACCACAGAGTCATTCTGTAGCTCATGATAAGACTCTGTGGTAATCA10.5586592178770949No Hit
GGTCGGTGCGGTGATGTGAACCGAGCGAACCCATCGACGACGAGGAACTC10.5586592178770949No Hit
AGTAACAGTTTACTCCAGGGGAAAGCTTTACTGATGAAAATCTTTCTGTG10.5586592178770949No Hit
CCCTTGGCACCGTGGACGCGGCATTCATCCAGAACCTGGCCATGAGCTTC10.5586592178770949No Hit
GAACATAGGTATGTGACCAAGACCAGGCGACTCCACTTCCCCATCCCCCT10.5586592178770949No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAG10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
TACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCT10.5586592178770949No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTATGCGTTGATACCA10.5586592178770949Clontech SMART CDS Primer II A (100% over 21bp)
GTTCTTGAAAACTTTCACTAAAGGGATTGTTGTCATCAAATTCTACCCGA10.5586592178770949No Hit
CCATGTGTACGGTGATGCGCTGGGTGGCGCCGGCCGACCCTGGCTCCGCG10.5586592178770949No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAAAC10.5586592178770949No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTGGGTTTATACCACTGCTTCCCATG10.5586592178770949No Hit
TATCAACGCAGAGTACATGGGATGATACGGCGACCACCGAGATCTACACT10.5586592178770949Illumina Single End PCR Primer 1 (96% over 30bp)
GAAACAAAACAAAACAAAAGTTCAGAAAATCATACATTATTTTACCATTG10.5586592178770949No Hit
CTCTCACTTTTCAAAAATACATCCTGTAATAATTTGGCTTCATATACAAA10.5586592178770949No Hit
AACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTG10.5586592178770949No Hit
GTTGTTCGGGGTGCCGTCCTCGTTCTCCGGGTGGGCGCCGCTGATGTTGG10.5586592178770949No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.5586592178770949Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCCTCGCCCGTCACGCACCGCACGTTCGTGGGGTACCTGGCGCTAAAC10.5586592178770949No Hit
CCCCCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGGCGCTAAAA10.5586592178770949No Hit
GAGTTAGGGAGGAGTCCCTCTTTCTATTGTTTGGAATAGTTTCAGAAGGA10.5586592178770949No Hit
GGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCT10.5586592178770949No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.5586592178770949Clontech Universal Primer Mix Long (95% over 21bp)
CGCTTATCCCACAGCACACACCCAAGGGTTCTGTTGCTAACACGCTGAAT10.5586592178770949No Hit
CTAGAAGTACTTGCTGAGTTTCTTGAATCTGTGCATTCGTTTCTTTCATC10.5586592178770949No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTGGTTTTAAAAAA10.5586592178770949No Hit
GGTTTTGGCCACATTGACTCTGTCTCATCTGCCCAGCACCAGCTCAGTGG10.5586592178770949No Hit
CATCCCAGGTAGCCCGCCTCCAAGAGCACTACACTCCCCCCAGCCCCATC10.5586592178770949No Hit
TATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTCTGTCT10.5586592178770949Clontech Universal Primer Mix Long (96% over 26bp)
TGTTCTAAGAGTTATTTTCTCTTTGGTTCCTCCAACACTTTGCTTTCCTG10.5586592178770949No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)