FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73.867403314917127Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT52.7624309392265194Clontech Universal Primer Mix Long (96% over 26bp)
GTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCG31.6574585635359116No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31.6574585635359116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31.6574585635359116No Hit
ACATGTATGACCATCTGGCGATGGGAGGCCATTCTTTTCAACAAGGAGAC21.1049723756906076No Hit
AATAAAACAAAGGCAGTTTTGTGAGAGTTTTGATGGAAAACCATTACATA21.1049723756906076No Hit
GAGTGTAGATCTCGGGTCGTATATCCCATGTACTCTGCGTTGATACCACT21.1049723756906076Clontech SMART CDS Primer II A (95% over 22bp)
CCAGTGTCATTGGTGAAGGCCTTGGGATTTGAGGCAGCTGAGTCCTCACT21.1049723756906076No Hit
GGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTC21.1049723756906076No Hit
TTCCCTACACGACGCTCTTCCGATCTCTGTACCTGCAGAACTTGGGACAG21.1049723756906076Illumina Single End PCR Primer 1 (100% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT21.1049723756906076Clontech SMART CDS Primer II A (100% over 26bp)
GGCAGCGTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAA21.1049723756906076No Hit
GAAGAGCGTCGTAGGAAAATGTATCTGGGCGTATCCCTGTCTCTTATACA21.1049723756906076No Hit
CTCTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGCGTCGTGTAGGG21.1049723756906076No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21.1049723756906076Clontech Universal Primer Mix Long (96% over 26bp)
GGCTGAAGATGTTAGAATAACCAATGTTTCCATTTAAATCTACAGGCAGG21.1049723756906076No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCT21.1049723756906076Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT21.1049723756906076Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCA21.1049723756906076No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGT21.1049723756906076Illumina Single End PCR Primer 1 (100% over 30bp)
CGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCCGGGACCGGGGTCCGGTGC21.1049723756906076No Hit
GGGGCGGCCCGCCGGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAA21.1049723756906076No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
CACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGAT10.5524861878453038No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATAG10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC10.5524861878453038No Hit
TCCAGGGTCTGCCCGAGGGTGATTGGTTCATCGCCCGAACCGGTTATACC10.5524861878453038No Hit
GCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCC10.5524861878453038No Hit
TCTGCGTTGATACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
TCCAGAACGTGCTCACCAGTGCCCGCAAGAAGCCGCCATCTGTCTCTTAT10.5524861878453038No Hit
GGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCT10.5524861878453038No Hit
CTATTGGATTTGGTAAGAAAATTTCATTTTTGCTATATATTCACTGATTT10.5524861878453038No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGATCACCCATGTACTC10.5524861878453038Illumina Single End PCR Primer 1 (97% over 35bp)
CCCTGGTGCCCGCGCTCGCTACCAGAAATCCTACCGATAAGCCCATCGTG10.5524861878453038No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTCTCTTATA10.5524861878453038Clontech Universal Primer Mix Long (96% over 25bp)
GTACTGGGTGATACGGCGACCACCGAGATCCCTGCTGGTACCAGTAGACA10.5524861878453038Illumina Single End PCR Primer 1 (95% over 22bp)
GAATCAATCCGTCCTCCTCCCCTTAAGGGCTAACCACTGGTTAATGAATT10.5524861878453038No Hit
CCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCG10.5524861878453038No Hit
TTGAATTTCTTCACTTTGACATTCAGAGCACTGGGCAGAAATCACATCGC10.5524861878453038No Hit
GATTAATCCCATCTGGCAAGATGGAGTGAGTTCCTGCTCTTGTGAGACTA10.5524861878453038No Hit
GGTCCTGAAAAACGAGCGTCGTGTAGGAAAGAGGTAGACTCCCCATGTAC10.5524861878453038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10.5524861878453038No Hit
GAGTACATGGGTCCGTATTAAGCGACATTCCTACGACTTATCAGGAATGT10.5524861878453038No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTACCTGTCTCTTATACACAT10.5524861878453038No Hit
GTTCTATAAACGAAAAGAACATGGACAGCAGTCTAATAAACACATATAAC10.5524861878453038No Hit
GGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT10.5524861878453038No Hit
GAATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAGTACTTT10.5524861878453038No Hit
GTACATGGGAATGACGCCCGATCCTTTCCTACAGCAACTCCAGTCCTTGC10.5524861878453038No Hit
AGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGAGCAGATCTTGGTG10.5524861878453038No Hit
AACGTATTTAGCCATTACCCACGTGACACCACCGAATATATTACTGATTT10.5524861878453038No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5524861878453038No Hit
CATTTGGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCACT10.5524861878453038No Hit
GTACATGGGAAGCAGTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT10.5524861878453038No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA10.5524861878453038No Hit
CACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTATCAACGCAGAGTAC10.5524861878453038No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC10.5524861878453038No Hit
CCCATGTACTCTGCGTTGATACACTGCTTCCCATGTACTCTGCGTTGCTG10.5524861878453038No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACA10.5524861878453038No Hit
CAATTGGCGCAGAGCCCATTCCAGCCTCGTTTGAAAACAGCGTGCGCTGA10.5524861878453038No Hit
CTGTAGATGAGTGACACTCAGCGTTTTAAAACCCTCCAATCGCTTCCCAA10.5524861878453038No Hit
GTACATGGGACGCTTCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTT10.5524861878453038Illumina Single End PCR Primer 1 (96% over 25bp)
CTTTAGGCCCTTTTTGTTGTGCTTCTTGGCAAAGCGCATGTTCCTCAGGA10.5524861878453038No Hit
GACACATTACCTGAATGAGCAGGTGAAAGCCATCAAAGAATTGGGTGACC10.5524861878453038No Hit
ACGCAGAGTACATGGGTGACGGCCACGAGTTCACTCTTTCCCTCACGACG10.5524861878453038No Hit
CCGTTAGGAAACACTCTGTTTGTAAAGTCTGCAAGTGGATATTCAGACCT10.5524861878453038No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG10.5524861878453038No Hit
GTAATCTCGGTGCGCGTATCACCCATGTACTCTGCGTTGACTGTCTCTTA10.5524861878453038No Hit
CTATAAACCTCAGGTAGAATATTTGAGAAAAAAAGGCATTGATGTTAATG10.5524861878453038No Hit
GTGTAGATCTCGGTGGTCGCCGTATCACCCCCCCCCCATGTACCGGTCGC10.5524861878453038Illumina Single End PCR Primer 1 (96% over 27bp)
GTTTTACAATCTTTGTCGGTTTAATAATCTCAACAATCTCAAGTACTGTC10.5524861878453038No Hit
AAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACCTGTCTCTTA10.5524861878453038No Hit
GTATCTAATACACAACACACAACACAATACAAACACAAAGAACAAACTAT10.5524861878453038No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTAT10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
GCTCAGCTGAAGAAGCTGGTGGACACCCTCCCCCAAAAGCCCAGAGAAAG10.5524861878453038No Hit
CGGTGGTCGCCGATCTCCCATGTACTCTGCGTCTGTCTCTTATACACATC10.5524861878453038No Hit
CACATGCTCCGTGGCCAACCCCTCCCCAAACATGGTGCCCCCATGAGCGA10.5524861878453038No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATACCACAGCGTAC10.5524861878453038No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
CAATTACCATAAAAAAATCTCAAACCATAAAATTAAACACAACTACGATC10.5524861878453038No Hit
CTGAAGCTCCTGCTGAGCAGCCTGGGCATCCCCGTGAACCACCTCATAGA10.5524861878453038No Hit
GTGCTGGGACAGGACAGCATCCTCAATCAATCCAACAGCATATTCGGTTG10.5524861878453038No Hit
AGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAG10.5524861878453038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT10.5524861878453038No Hit
GTCCCATTCCATCCCAATTCCAATCCTGACCCAGAAAGTGATGGTGGCAG10.5524861878453038No Hit
GTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACA10.5524861878453038No Hit
GTGTAGATCTCGGTGGTCGCCGTATCACCCCCCCCCCAGGGACCTGTCTC10.5524861878453038Illumina Single End PCR Primer 1 (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCCT10.5524861878453038Clontech Universal Primer Mix Long (95% over 21bp)
GTTAAAGACAGAGCTGTCTCTTTTGTAAATGCTTTTGGAATGAAGCAGGA10.5524861878453038No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTAATCTCGGTGGCGCCGTCATTCC10.5524861878453038Illumina Single End PCR Primer 1 (96% over 28bp)
ATCAACGCAGAGTACATGGGTATTCGTCGGCAGCGTCAGATGTGTATAAG10.5524861878453038No Hit
CCATTCGCTGATGATTGCATTCGAGTCCATGGATTCTTCCATTCCATTCC10.5524861878453038No Hit
GCTTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGAT10.5524861878453038No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.5524861878453038Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
ACTTGGGACAGAGCGTCGTGTAGGGAAGAGTGTAGACTCTGTCTCTTATA10.5524861878453038No Hit
ACCTGGTGCCCCCCGTGGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTG10.5524861878453038No Hit
CATAAGTTTAATGAAGTCTGAAAGGTAAGCGCCATCGTGGTCAGAGATAC10.5524861878453038No Hit
GCTCAGTTGATCCGGGAGACCGGGTCCGGATCGAACGACACCTGCAGTTC10.5524861878453038No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTCTTATACACA10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5524861878453038No Hit
ATGTAAGCGTTTTGTTGTTTTGTTTTGTTTTGTTTCTGTCTCTTATACAC10.5524861878453038No Hit
CTCTCGGGGCGAACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGA10.5524861878453038No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT10.5524861878453038Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACATCACGAGTAAGTTTCTGAGAATGCTTCTGTGTAGTTTTTATGGGAA10.5524861878453038No Hit
GGGTGGAACTCGGTGCTGCCGGTGCTGGCGGCAACGGCCACCTCGTTCCT10.5524861878453038No Hit
GTACATGGGAATGACGCCCGAACCATTCCTACAGCAACTCCATTCCTATC10.5524861878453038No Hit
GTATTCCTCAGCGCCGACGACTTAACGCCGGCCAGGCTGGAGCGCGAGGG10.5524861878453038No Hit
CCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTC10.5524861878453038No Hit
CAACGCAGAGTACATGGGACATTCAGACTGAGCGTGCCTACCAAAAGCAG10.5524861878453038No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGTTT10.5524861878453038No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT10.5524861878453038No Hit
ATCTACACTCTTTCCCTACACGACGCTTGCCTCGATTCTGTCGGTACCAG10.5524861878453038Illumina Single End PCR Primer 1 (96% over 27bp)
CTCTTGCTCTGAAGGTAAAAGACACTTCCTCTGGTTGAATGACTCTTGGG10.5524861878453038No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTT10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
TGGTAGCGGTATTCTCCGCGGCAGTGACAGTAATTGTTTTTGCCTCTTTA10.5524861878453038No Hit
CTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCG10.5524861878453038No Hit
GTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCC10.5524861878453038No Hit
CAACATGCAAAGAATTGATATCAACGCACAGTAACTTATAAAAATCACAG10.5524861878453038No Hit
TTCGACAGCTTCCGGCGCATTGCAGTATGAGCTGAAAGTAATTGGTGATC10.5524861878453038No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGGTGGTATC10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGTACATGGAAGCAGTGGTATCA10.5524861878453038Clontech SMART CDS Primer II A (95% over 23bp)
TTCTATTGCAGTCCATTCCATTCGAGTCCATTCCAATCCCTTCCATTCCA10.5524861878453038No Hit
CGTGTAGGGAAGAGTGAGACGGGTCGACCCATGTACTCTGGGTTGATACT10.5524861878453038No Hit
GAACGACGCAATCAATCCCGCAGCGGAACCCGAAGACGCCGAAGGCGGCG10.5524861878453038No Hit
GGAAAGTCAAAAGCATATACAGAGGAAGAGGTTTCTCCAAAACTCGTACA10.5524861878453038No Hit
GTTCTGGTATCGACAAGACCTGGGACAGATCGGAAGAGCGTCGTGTAGGC10.5524861878453038Illumina Single End PCR Primer 1 (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGATACCACGGCTTCC10.5524861878453038No Hit
GAGCAGAAGGTGGCACAGCCCGCGCTGCAGCACTTGAGGTTGTCGGCGCA10.5524861878453038No Hit
TCTACGAATGGGTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACG10.5524861878453038No Hit
TCGCAGCTGGGCGCCGATGTGGGCCTCGGCTACACCCAGCCGCAGCTGGC10.5524861878453038No Hit
GTACATGGGAATGCCCGAGATCACATTTCCCTACACGACGCTCTTCCGAT10.5524861878453038Illumina Single End Sequencing Primer (96% over 26bp)
CTACACGACGCTCTTCCGATCTGTACTGGTATCGACAAGACCCGGGAGAG10.5524861878453038Illumina Single End PCR Primer 1 (100% over 22bp)
GGGGTGGGGATAACTAAAAAGGAGTGCTTAAAAGAGTATTCCTGGACTAC10.5524861878453038No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT10.5524861878453038No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.5524861878453038Clontech Universal Primer Mix Long (95% over 21bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATA10.5524861878453038Clontech Universal Primer Mix Long (95% over 21bp)
AATTTCCCTCTTCCCCAAGGCAAGAGTGAGAAAGGCAGCTTTTCCCATTT10.5524861878453038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATA10.5524861878453038No Hit
GGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCG10.5524861878453038No Hit
TTTTTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGT10.5524861878453038Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTT10.5524861878453038No Hit
GAGTACATGGGCCGCCACCCGTCTACCTCATCCCTACACGACGCTGATCG10.5524861878453038No Hit
GTACTTGTGGAAGATGGCCACGAGGAGGCCAATGGCCTGATCCAGGGGGC10.5524861878453038No Hit
AATAATTGAAGGCTGCGTCAAAAAAAAGTTGATGAAATGGAAGAAGAGAG10.5524861878453038No Hit

[FAIL]Adapter Content

Adapter graph