FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42.209944751381215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31.6574585635359116No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31.6574585635359116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTACACGACGCTCTTCCGATCTCACTGTCTACTGGTACCAGCAGGGAAAG31.6574585635359116Illumina Single End PCR Primer 1 (100% over 22bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31.6574585635359116Clontech Universal Primer Mix Long (96% over 26bp)
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC31.6574585635359116No Hit
ATGGCTGATACGGCGACACCGAGATCTACACTCTTTCCCTACACGACGCT21.1049723756906076Illumina Single End PCR Primer 1 (97% over 35bp)
ATCAACGCAGAGTACATGGTATTAGGCGACACCGAGATCTACACTCTTTC21.1049723756906076Illumina Single End PCR Primer 1 (95% over 22bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCT21.1049723756906076Clontech Universal Primer Mix Long (96% over 26bp)
GGTTCAGACATTTGGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAG21.1049723756906076No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.1049723756906076Clontech Universal Primer Mix Long (96% over 26bp)
CGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGT21.1049723756906076No Hit
GACCCAGCCTTCCCTGATGGCTGCCATCCAAGTCTTCACTGGGAGACAGG21.1049723756906076No Hit
GGCAGATTTAGTAAAGCAGCTTGTCTTCCATAGTGTGAGTGGGCCTTATC21.1049723756906076No Hit
GCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGA21.1049723756906076No Hit
TGTTATGAGAGTGTTTTGAGAAAGTTAGACAAAGCTTTAGCAGAAAAACA21.1049723756906076No Hit
CCCCCCCCTCCACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGC21.1049723756906076No Hit
GCCTGGGCATCCCCGTGAACCACCTCATAGAGGGCTCCCAGAAGTGTGTG21.1049723756906076No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATGACGCCCG21.1049723756906076Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAATGTCGGCGACACGAGATCTATACTCT21.1049723756906076No Hit
CAACAAACAAACATCAAATACCCAAGCATGTTTTGGCTTGTACCGTAAGT21.1049723756906076No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT21.1049723756906076Clontech Universal Primer Mix Long (96% over 25bp)
GGATACGCCCAGATACATTTTCCTACGACGCTCTTCCTGTCTCTTATACA21.1049723756906076No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTCGCTAGACATACTGTAGATATATCTCGTCCTTGAAATGCTTTAGC10.5524861878453038No Hit
GCCTGGGAAACAGAGTGAGACTCCGTCTCAAAAAACAAAAAACAAAAACA10.5524861878453038No Hit
GGAGAAATGAGTAGAAGAAGATGGGGTTCAGGACACAGGTAGCGACGAGG10.5524861878453038No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT10.5524861878453038No Hit
GGTAAAATGGGGGAATTGTAAGGAGAGTTTATAGGCTTTAAAAGGCCATG10.5524861878453038No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT10.5524861878453038Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATGT10.5524861878453038No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGCT10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
GTGTTGTGTGTATTCAACTCACAGTCTGCTCTGTGTAAAGGATCGTTCAA10.5524861878453038No Hit
ACTGTGCCACCAGGCGGGTGGGGCCACTTGCACTGGTGTCCATGCGGATG10.5524861878453038No Hit
AGCTGGTACTCGTTGGAGCGTTCGTAGCAGGCACGCACTCCTTCATCCTC10.5524861878453038No Hit
GTGTTAGCCAGGATGGTCTCGATCTCCTGACCTTGTGATCCGCCCGCCTC10.5524861878453038No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGA10.5524861878453038Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCATGCAGTGGTCAAGTAGAAATGGGAAAAGCTGCCTTTCTCACTCTTG10.5524861878453038No Hit
TCTTTAGCCAAGACTTCCGCCCTCGATCAAGATGGTGGTTGGACGGCCTT10.5524861878453038No Hit
TACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGA10.5524861878453038No Hit
GTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATTATG10.5524861878453038No Hit
GGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGA10.5524861878453038No Hit
GTACATGGGTGGCGAGCTTCCCAACACAGCAACTCCAGTCCTTGCATCAT10.5524861878453038No Hit
GTACATGGGGGGGGGGTGATACGGCGACCACCGAGAAATACACCTGTGTC10.5524861878453038No Hit
GAGTAATCTGGGACATCTTAGTGTTTTGTTTTGTTTTTTTCCCTCCTCTT10.5524861878453038No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATATGCGTTTAAA10.5524861878453038No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTACAAC10.5524861878453038No Hit
ACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGCCGGG10.5524861878453038No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10.5524861878453038No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGGGGTTTTAACC10.5524861878453038No Hit
CTTGCGTGCAGTTCTGGTCAGCCTGGAGCTCGGGGCACACGCCAGTCTTC10.5524861878453038No Hit
TCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG10.5524861878453038Illumina Single End PCR Primer 1 (100% over 22bp)
GTACATGGGGGGGGGGTGATACGGCGACCACCGAGATCTACACCAGTCTC10.5524861878453038Illumina Single End PCR Primer 1 (96% over 27bp)
AGGTGAAGCGCGAGCCGGGCACGGTCGCGCCCTCGCGCTCCAGCCTGGCC10.5524861878453038No Hit
GTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAA10.5524861878453038No Hit
CTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGGA10.5524861878453038Illumina Single End PCR Primer 1 (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATT10.5524861878453038Clontech Universal Primer Mix Long (95% over 22bp)
AAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCG10.5524861878453038No Hit
ATTTTTTTCTGGCAGAGTTTAAATGCACGTAATGGATAATTCTGAAGAAA10.5524861878453038No Hit
CGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCCGGGACCG10.5524861878453038No Hit
GGTTGAATGACTCTTGGGAAACCTATGTTAGTTTTTGTTTCCCAAGAGTC10.5524861878453038No Hit
ACACCTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGAGA10.5524861878453038Illumina Single End PCR Primer 1 (95% over 21bp)
GTACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT10.5524861878453038No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT10.5524861878453038No Hit
GAGTACATGGGAATGACGGCGCCACCGAGATTACACTCTTTCCCTACACG10.5524861878453038No Hit
GTCAGATACTGAGTGGAGGGCTATGCACTATTAATTCCTCTGAGTTGTGC10.5524861878453038No Hit
GTCAGTGCAGAGGGAGAGTCTGACCGGGCCTCCGTATCTCTGACCACGAT10.5524861878453038No Hit
AACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCC10.5524861878453038No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAGTACCT10.5524861878453038Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATCGGCGAC10.5524861878453038Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTTCACTCTTTCCCTCACGACGCTCTTCCGATCTGTTCTGGTATCGACA10.5524861878453038No Hit
AGTCTACACTCTTCCCTACACGACGCTCTGTCCCAAGTCTGTCTCTTATA10.5524861878453038No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC10.5524861878453038No Hit
TATCAACCCAGAGTACATGGGTCGACCCGTCTCACTCTTCCCTACACGCT10.5524861878453038No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTT10.5524861878453038Clontech SMART CDS Primer II A (100% over 26bp)
CTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTC10.5524861878453038No Hit
CCACAAGCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.5524861878453038No Hit
AAACAAAACAAAACAAAACAACAAAACGCTTACATCTGTCTCTTATACAC10.5524861878453038No Hit
CCTTGGCGGCGGCCGGCTCGCCGCTTTCCAGCTGGTTCTCGATGGCCTGG10.5524861878453038No Hit
CTTTAAAATCCCTCAAAAACTGTTTATTATACAAGTGAGTTTTGAGTCAC10.5524861878453038No Hit
GTACATGGGAGCACCACGGATACTCTTCCCTCACGCCTCGTTTTCAGGAC10.5524861878453038No Hit
CCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACA10.5524861878453038No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACTGGGTGATACGGCTCTTCC10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
AAACCATTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAG10.5524861878453038No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTCCGTATTAAG10.5524861878453038Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACGAGATCTACAC10.5524861878453038No Hit
GAATCATGATGAAATGGAATCGAATGTAATCATCATCAAATGGAATCAAA10.5524861878453038No Hit
ACGCAGAGTACATGGGAGATCGGCGACCACCGCTGTCTCTTATACACATC10.5524861878453038No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.5524861878453038Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAGCAGAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAAACCCTTGTGT10.5524861878453038No Hit
ACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAG10.5524861878453038No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATTGGGTTTTAAACA10.5524861878453038No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.5524861878453038Clontech Universal Primer Mix Long (100% over 23bp)
GCATTACATTTGGAAAAAAATGTGAATCAGTCACTACTGGAACTGCACAA10.5524861878453038No Hit
GAATAGAATGGAATGGAATGGAATTGACTCAAATGGAATGGACTAGAATG10.5524861878453038No Hit
CACTTTCCCACCCTATCCTTCCTCCCCACTCTGTGGACTTGAAATTGAAT10.5524861878453038No Hit
ATGGCGGCTTCTTGCGGGCACTGGTGAGCACGTTCTGGACTGTCTCTTAT10.5524861878453038No Hit
GATCGTTGAAGAAAGACACCACCTCGGTCACCGGCAGCACGATTTCCAGA10.5524861878453038No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATGCTTTTAAACAAAT10.5524861878453038No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTTGGGTGTCTTGAAGCCCAGACCGATGTTCTTGTAGTACCGCGGGA10.5524861878453038No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTATACACA10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
GTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAA10.5524861878453038No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.5524861878453038Clontech Universal Primer Mix Long (95% over 22bp)
GTGAAAAAGGACATATCTTCCCATAAAAACTACACAGAAGCATTCTCAGA10.5524861878453038No Hit
TCAACGCAGAGTACATGGGTGATACGCGCACCGAGATTACCTGTCTCTTA10.5524861878453038No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.5524861878453038Clontech Universal Primer Mix Long (95% over 22bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATA10.5524861878453038Clontech Universal Primer Mix Long (95% over 21bp)
ACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGAC10.5524861878453038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.5524861878453038No Hit
TGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGCT10.5524861878453038No Hit
TCCCCCGGCCCCTCTCAATAAACGTGGTTAAGAGCAAAAAAAAAAAAAAA10.5524861878453038No Hit
CAACGCAGAGTACATGGGAAGCAGTGTATCAACGCAGAGTACATGGGCTG10.5524861878453038No Hit
CGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTCCCGGGTC10.5524861878453038Illumina Single End PCR Primer 1 (97% over 42bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCTGTCTCTTATA10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
TACTTGAGATTGTTGAGATTATTAAACCGACAAAGATTGTAAAACCTGTC10.5524861878453038No Hit
CCCAAGTTCCTGAGGAACATGCGCTTTGCCAAGAAGCACAACAAAAAGGG10.5524861878453038No Hit
CTTTTTAAGCTGCGTTGTCATAATTGCTACAAACTACGATAAGTTGGGCA10.5524861878453038No Hit
CAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATG10.5524861878453038No Hit
GCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAA10.5524861878453038No Hit
GCCGAGGAGGTGCCCTGCGCCAGATCGCCCTGCTCAAGCAGCAGGACGCG10.5524861878453038No Hit
CCCCCTGGATCAGGCCATTGGCCTCCTCGTGGCCATCTTCCACAAGTACC10.5524861878453038No Hit
GGATGAGATCCACGTCGCCGACCGCGCCCTGCACGGTCTTGTTGAGCGAC10.5524861878453038No Hit
CCAGTAGGCGATGCGCTTAATGTTACCGGATCATCGCATGGCTGGCTCTG10.5524861878453038No Hit
CTACAGCTGCGAGCGGGGCATCTGCTATTTCTCGGAGTCGGGCTCTGTTG10.5524861878453038No Hit
TTCTTGTATGGAAAATATTCTTATTTTTCCCAATCCTGCTTCATTCCAAA10.5524861878453038No Hit
ACAACACATAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAA10.5524861878453038No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
ATCTAAGAGTTACCTGGCTACAGAAAGAAGATGCCAGATGACACTTAAGA10.5524861878453038No Hit
ATCGGAAGAGCGTCGTGTAGGAAAAGTGTAGTCTCGGGGCGCCTACATCC10.5524861878453038Illumina Single End PCR Primer 1 (96% over 26bp)
CTCTTATACACATCTGACGCTGCCGACGAATACCCATGTACTCTGCGTTG10.5524861878453038No Hit
GAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCG10.5524861878453038No Hit
TAATTAAACAGTCGGATTCCCCTGGTCCGCACCAGTTCTAAGTCGGCTGC10.5524861878453038No Hit
GCACGAAGAACAGGATCCAGGCCAGGTAGACAGAACCAGCGAGAGACACC10.5524861878453038No Hit
GTACATGGGAAGCAGGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT10.5524861878453038Clontech SMART CDS Primer II A (100% over 21bp)
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG10.5524861878453038No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGCTGTCTCTTAT10.5524861878453038Clontech Universal Primer Mix Long (96% over 26bp)
GCACAAACACAACCAGCAGAGTCCCTGGCAGTTCCAGGCAGTGTTTGCTC10.5524861878453038No Hit
GTGGTATCAACGCAGAGTACATGGGTACGGCACCACCGTGATCTACACTC10.5524861878453038No Hit
AACGCAGAGTACATGGGAATTTGGTACCCAAAAAAAAAAAAAAAAAAAAA10.5524861878453038No Hit
CTGTCCCAGGTCTTGTCGATACCAGAACAGATCGGAAGAGCGTCGTGTAG10.5524861878453038Illumina Single End PCR Primer 1 (100% over 22bp)
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTCTGTCTCTTA10.5524861878453038No Hit
GTACATGGGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA10.5524861878453038No Hit
GATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA10.5524861878453038No Hit
TCCCTACACGACGCTCTTCCTGTCCCAAGTTCTGCAGGTACCAGAGATCG10.5524861878453038No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG10.5524861878453038No Hit
GGCGCAGCGTGCCGTCGAGCAGCTTGCGCAGGAAGCGTGCGTAGTCGGCG10.5524861878453038No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAACTGT10.5524861878453038Clontech Universal Primer Mix Long (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)