FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG83.4482758620689653Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73.0172413793103448Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT41.7241379310344827Clontech SMART CDS Primer II A (100% over 26bp)
GTTAAAGTCTCCCATTATTATTGTGTGGGAATCTAAGTCTCTTTGTAGGT31.293103448275862No Hit
ACTGAAGTGTTGCAGCTGTCCCCGCCAGACCGATCATCGGTTCATCGGCC31.293103448275862No Hit
AATCCCAGCTACTCAGGAGGGTGAAGCATGAGAACTGCTTGAACCTGGGA31.293103448275862No Hit
GGCTAATTTTTGTATTTTTAGTTGAGACGGGGTTTCACTATGTTTGCCAG31.293103448275862No Hit
ACCTTATTTACCCGGCCACTGTTCATCTCATTAAGGAATGATCCAATGTC20.8620689655172413No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
CTGCAATTCATGAAATTCCCTGTCCCCATGGCAACCCAAATCCAACAACT20.8620689655172413No Hit
GTAATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATA20.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACT20.8620689655172413Illumina Single End PCR Primer 1 (100% over 33bp)
ATCTAATAAAGAGAACCCACGTGCCCCAGGGAGGAGAAGCCCTCTGTGTG20.8620689655172413No Hit
ATGGCCAAAATCCCAACTGTAGAACCATCCTCAGAGAACTTGTCATGTAA20.8620689655172413No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG20.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTTGGACGCTTGAGGAAAACGCCATTCCGATCGGCATACGGAAGGCGGT20.8620689655172413No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT20.8620689655172413Clontech SMART CDS Primer II A (96% over 26bp)
AAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTATTAG20.8620689655172413No Hit
AGGGAGAGAAGTGAAATAGCGGAGCACCCAGCCCGTGCCATGGGAGCTGA20.8620689655172413No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAGTACTC20.8620689655172413Clontech Universal Primer Mix Long (95% over 21bp)
GTAAAGGCGAGTTCACAGTTTCATCAACTCTATACGGAAATGCACCAGCC20.8620689655172413No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT20.8620689655172413Clontech Universal Primer Mix Long (95% over 21bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.8620689655172413Clontech Universal Primer Mix Long (100% over 23bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
CCCCGGGTCTTGTCGATACCAGTACAGATCGGAAGAGCGTCGTGTAGGGA20.8620689655172413Illumina Single End PCR Primer 1 (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.8620689655172413Clontech Universal Primer Mix Long (95% over 22bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT20.8620689655172413Clontech SMART CDS Primer II A (100% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
GGATAAGGGGCCACACACCAGCGGCGGTTTGAATGCCATGTGTTTCCCGG20.8620689655172413No Hit
TTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCC20.8620689655172413No Hit
GCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG20.8620689655172413No Hit
ACATGGGAAGCAGTGATATCAACGCAGAGTACATGGGAACGACCCCGAGA20.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGCCAAGAAGAGTAGCGACCCTCTGTCTCTCTTCCAGGAACTGAGCCTAG20.8620689655172413No Hit
GAGTACATGGGTGATCGGGACACCGAGATCTCCTCTTCCCTCACGACGCT20.8620689655172413No Hit
GTACATGGTCGGACCACCGAGACCACTCTTTCCCTGGACTGGAGTTGCTG20.8620689655172413No Hit
ATCCTGGTAGGTTGAAAACCAGACAGAAAGAAGTTCTCTGAAATTTATTC10.43103448275862066No Hit
ATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG10.43103448275862066No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
ACTACACTCTTTCCCTACACGACGCTGATCGGAAGAGCGTCGTGTAGGGA10.43103448275862066Illumina Single End PCR Primer 1 (96% over 26bp)
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC10.43103448275862066No Hit
GATAAAAGGTTCCAGATGAATATCAACGGCTTCCTGAAGAGAGACCGGGA10.43103448275862066No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGGTATCAACGCAGAGTACATG10.43103448275862066No Hit
TTCCCATGTACTCTGCGTTGATACCATGCTTCCTGTCTCTTATACACATC10.43103448275862066No Hit
GGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCT10.43103448275862066No Hit
GGCGACCTTCGAGCAGCGCTTCCCCGACGCTGGCGGCTTCTACACGGTCC10.43103448275862066No Hit
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA10.43103448275862066Illumina Single End PCR Primer 1 (100% over 21bp)
GGCCGTGCGTGTAGACGTATTTGTAGTGTCCCTTGCGCAGCATGCAGCAT10.43103448275862066No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTATACGGCGACCACCG10.43103448275862066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA10.43103448275862066No Hit
CTCGGTGGTCGCCGTATCATCCCATGTACTCTGCGTTGATACCACTGCTT10.43103448275862066Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAT10.43103448275862066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10.43103448275862066No Hit
GTTAAAGTCTCCCATTAATATTGTGTGGGAATCTAAGTCTCTTTGTAGGT10.43103448275862066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.43103448275862066No Hit
CGCCACCGGATGTAATGCCGAACAAGCGGAAGCGGCGTTAATTGCCTGCG10.43103448275862066No Hit
GGTATATGCACACTGAAAAGCTCAGTAGGGTGATTTTGATGCTAAGTTAT10.43103448275862066No Hit
GTACATGGGTGACGGCCCCCGAGATCTACACTCTTTCCTACACGACGCTC10.43103448275862066No Hit
AACCTGCACAGATCGGAGCACCATGGCACGTGAGATCGGCATCGGAGTGG10.43103448275862066No Hit
GATTTTGCAAATGTCAGCACGTAAATAATGCCTATTATTAGGAAAATCAT10.43103448275862066No Hit
ACTCAGGAGGCTGAGGGGAGGATCACTGGAGCCCAGGAGTTTGAGGCTGC10.43103448275862066No Hit
GCTGAAACCTGAATATTGTTGAATAAAGGCCTGAAGAAGACCTTTTCTTC10.43103448275862066No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.43103448275862066No Hit
GCGTTAGGGATCAGTCGCGGCACGCTGTACCGGCGCATGGCCCAATATGG10.43103448275862066No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACT10.43103448275862066No Hit
CTTCTGTCTTGCTTCGCCTTTTGTGTTTTGTCTCTTTGTTGATGATGGCA10.43103448275862066No Hit
AAAGAAAATGAAAACAAAAGGCTTCTCCTGAGCAGCCATAGTGGACACCC10.43103448275862066No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA10.43103448275862066Clontech Universal Primer Mix Long (95% over 21bp)
TCTCGGTGGTCGCCGTACACCCATGTACTCTGCGTTGATACCACTGCTCC10.43103448275862066Clontech SMART CDS Primer II A (96% over 25bp)
GTAATCCCAGCACTTTGGGGGGCCAAGGCAGGCGGATCACAAGATCAGGA10.43103448275862066No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGTGGGGG10.43103448275862066No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTAC10.43103448275862066Clontech Universal Primer Mix Long (95% over 21bp)
CGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCC10.43103448275862066No Hit
GAGGCAAGAGCGTCGTGTAGGAAGAGTGTAGTCTGTGCCCCATGTACTCT10.43103448275862066No Hit
GTACATGGGAATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACA10.43103448275862066Illumina Single End PCR Primer 1 (100% over 41bp)
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.43103448275862066Clontech Universal Primer Mix Long (95% over 21bp)
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG10.43103448275862066No Hit
ATGCCGCGCTGGCGCTTCCACCCTCGGAAAGCGCATGTTCGGTGTAGTCG10.43103448275862066No Hit
CTCCTTGGCACCAGTGATCAGGCTCCCAAGTCTGGTTACCATGAGGTGAG10.43103448275862066No Hit
CGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAGCGTCGTGTAGGGAA10.43103448275862066Illumina Single End PCR Primer 1 (100% over 26bp)
GAGTTCTACAGAAATATTCAACCAGGAAATTACCCAGTGACATTCTGCAG10.43103448275862066No Hit
CATGGGATGACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGC10.43103448275862066Illumina Single End PCR Primer 1 (100% over 41bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGAT10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTC10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGA10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
GTCACTGTCTGTGTCACGTGGATCTCGGGTCTGCTCTGCTTGACACCGCT10.43103448275862066No Hit
ACCTGCTTCTGGGTCGGGGGGTGGGTCGGAGCAGCGCCGCGCCCCCGCGG10.43103448275862066No Hit
GGTATAAACATAAACCATGATATGTTCAAGGGGAATACTGGTGGTTCACT10.43103448275862066No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGTAGATCTCGGTGGTCGCCGT10.43103448275862066Illumina Single End PCR Primer 1 (96% over 29bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.43103448275862066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCGAGGGTCGGTCGCACCGATCTTTTTAGCATCGACCTCCTCGCGCGCC10.43103448275862066No Hit
GTTAAAGGGTATTCCAAACATCAAGGCTTTTAATACATACTGCCAAACTT10.43103448275862066No Hit
CCGTATCACCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTG10.43103448275862066No Hit
TCCCTACACGACGCTCGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGA10.43103448275862066No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATACACATC10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
CGCCGAGATTACACTCTTTCCCTACACGACGCTCTTCCGATCTCATGTTT10.43103448275862066Illumina Single End PCR Primer 1 (100% over 34bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
GGCATGAACACGTCCGCGGCGGCCATCACCGATTCGACGACACCGGCGAC10.43103448275862066No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGCCCCC10.43103448275862066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTTCCAAAGTGCTGGGATTGCAGGTGTGCACCCGCCTGAGCCACTGCG10.43103448275862066No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCT10.43103448275862066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATATGCACACTGAAAAGCTCATTAGGGTGATTTTGATGCTAAGTTAT10.43103448275862066No Hit
GTCTACATCTTTCCCTACACGACGCTGTCCCAAGTTCTGCAGGTACCAGA10.43103448275862066Illumina Single End Sequencing Primer (95% over 21bp)
GGAATGAAATCTGTGAGAGAATATTTTTGGTTCTAAAGACGGGTGCATCC10.43103448275862066No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT10.43103448275862066No Hit
CCTCTCCCTCTGACACAGTTCACAAAATATTCAAAGGGAGCACCTAACAA10.43103448275862066No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC10.43103448275862066No Hit
GACGAGCGCGGCGAGCGCCGCCGCATCTGGCGCGCCCGCGTGGGCGACGA10.43103448275862066No Hit
ACATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
GCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTG10.43103448275862066No Hit
CTCACACATACACACAGAAAAAAAAATGCTTAAAGCAACTTTCCCCAAAA10.43103448275862066No Hit
CCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAGG10.43103448275862066Illumina Single End PCR Primer 1 (100% over 23bp)
CCGCAGGCCCTTGAGGGCCGCCTTGACCACCATCTCGCTGGCGAACTCCA10.43103448275862066No Hit
ATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.43103448275862066Clontech Universal Primer Mix Long (96% over 27bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.43103448275862066Clontech SMART CDS Primer II A (96% over 26bp)
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA10.43103448275862066No Hit
CATCTTCGTTGTTCTTGATGAAAGAGTTCAGCGGCCCGAGAACCGCGCCG10.43103448275862066No Hit
GGTCCTCGGCGACCTCGCGGGAGCCCAGCAGGAAGGTCATCTCGTTGCCG10.43103448275862066No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAAGGGAAGC10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGCGGTGTAGGGA10.43103448275862066No Hit
GCATGACCCAGTCACCTCCTTAACACTATTGCATTGGAGATTAAGTTTCA10.43103448275862066No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACTGCTTCC10.43103448275862066No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATGAG10.43103448275862066No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.43103448275862066Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAACTGTCTCTTATACACATC10.43103448275862066Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGA10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGTGTCTGTCTCTTA10.43103448275862066Clontech Universal Primer Mix Long (96% over 25bp)
CCCTACACGACGCTCGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAG10.43103448275862066No Hit
CGTAAGGCCTTTTGGGCATAATGAATTAGGAGGCGGGGAAGAGGGACTTT10.43103448275862066No Hit
CACCCACGCCGAACGGATGCTCGCACCGCCGCTCAGCGCCCGCTTCATCC10.43103448275862066No Hit
AAGTGATTCTCTTTGAGAAATAGGAAATGACTCTCCAAATAATGTTAATA10.43103448275862066No Hit
TATCAACGCAGAGTACATGGGAAGCACTGGTATCAACGCAGAGTACATGC10.43103448275862066Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAAG10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
ATCTGAGGCAAGAGCGTCGTGTAGGGAAAGAGTGTAGTCTCGGGGCGCGT10.43103448275862066Illumina Single End PCR Primer 1 (96% over 28bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGCTGTCTC10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
GATCTACATCTTTCCCTACACGACGCTCGTTTTTCAGGACCTGTTGGTAC10.43103448275862066Illumina Single End PCR Primer 1 (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTAAAAAAATTAAAATAAAAAAAA10.43103448275862066No Hit
CATTTCATCTCAGCCCCATGTACTCTGCGTTGTGGTATCAACGCAGAGTA10.43103448275862066No Hit
GGATAAAAGAAGCAAAATTGAAGTATTCTGTCTTACAGTATATGAGAAGT10.43103448275862066No Hit
TATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.43103448275862066No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGTTTTAAAACCAA10.43103448275862066No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.43103448275862066Clontech SMART CDS Primer II A (96% over 26bp)
CATGGGGACTCCTACAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGG10.43103448275862066No Hit
TTGCCTAGATGTCCCCGCTTCCTTTGCTAACAGATATTTGGAGGATGAAA10.43103448275862066No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTATACGGCG10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
TCTATGAACTGTCGGCCGAGCATCATTTCAAGAACGAGATCGTGCTGCGC10.43103448275862066No Hit
AGCTTGATGTGGGCTGATTTTAAAAGAATAAATGAAAAAAAAAAGCTGGT10.43103448275862066No Hit
TTATGGTTTTAGGTCTAACGTTTAAATCTTTAATCCATCTTGAATTGATT10.43103448275862066No Hit
GCAGAGTACTTTTTTTTTTGGTTGATTCTACTAGTAACAATTTCTGAATC10.43103448275862066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10.43103448275862066No Hit
CCCCTCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGGCGCTAAACC10.43103448275862066No Hit
GGCCATCACGAGTAGCCAGGGCGTCACCCGGCTGAAGATCTCGGCGCCGA10.43103448275862066No Hit
GCATCCGAGAGGGGATCGGGTTCGGGGCCTGCCGTCGGGGTCGGATCCGC10.43103448275862066No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGCAGTCTT10.43103448275862066Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT10.43103448275862066Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCGTAA10.43103448275862066No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTAAAAAATAATATATGTATTTAAAT10.43103448275862066No Hit
CTTCCCAACTTGCCTTTGAAGAGTGAAAAAACATTATTATCAAGTAGACT10.43103448275862066No Hit
CTGCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC10.43103448275862066Clontech SMART CDS Primer II A (96% over 26bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCGTTTTAAA10.43103448275862066No Hit
CACGTGCACAGCCATGTTCTCGACGATGCCCAGGATCGGCGTGCCGATCC10.43103448275862066No Hit
ACTACATCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCGACAGAA10.43103448275862066Illumina Single End PCR Primer 1 (100% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCC10.43103448275862066Clontech Universal Primer Mix Long (95% over 21bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.43103448275862066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCACATACCAGACTGAATGTTGGCAGGAGGAGTGCGTGAAGCAAGAAC10.43103448275862066No Hit
ATCAACGCAGAGTACATGGGGAAGCAGTGGTAACAACGCAGAGTACATGG10.43103448275862066Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.43103448275862066Clontech Universal Primer Mix Long (96% over 26bp)
CTCAAGTCTTGCTAAGCAGAGATATTAAATTAGATTTTTCAGTTCTTCCA10.43103448275862066No Hit
GCCTCCTGCAGTCTTTTCTCCCCGATGGATCAGGCATTACACACAGAAGC10.43103448275862066No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAACGGCGTTTAAA10.43103448275862066No Hit
GGAAGATGCTTCGTTCTAGTGGTCACGGACGGTGCAAGCTCTGTCTCTTA10.43103448275862066No Hit
CCCATTCAGTGCAAAAAAAAAAAAAAAAAACAATAACAACAAAAAAAAAA10.43103448275862066No Hit
CCCCGGAGCTCACCGCCAGCACGACCGTGGCCCAGGCCAGCGCATCCATG10.43103448275862066No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATACGCGAA10.43103448275862066Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)