FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115
Sequences flagged as poor quality0
Sequence length140
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA32.608695652173913No Hit
TTACGATAGGGTGTGGTGCCGTGAAGCACTGTGGCCATTTTTAACAAGCC32.608695652173913No Hit
AAATTAGTTTTTACTAAGGCTGGGCAAAGTGGCTCATGCCTGTAATCCCA32.608695652173913No Hit
TGATTCCATTCGATTCCATTTTATGATGATTCCATGCAATTCCATTAGAT21.7391304347826086No Hit
CATCACGGGCGAGGACCCGCTGGCGCGCAAGTTCGCGATCGGCTACGTGC21.7391304347826086No Hit
GATAAAAACTACACAGAAGCTTTCTGGGAAACTTCGTTGTTAGCTGTGCA21.7391304347826086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.7391304347826086No Hit
GCCCTACACGACGCTCTTCCGATCTCTAAACCTGTCTCTTATACACATCT21.7391304347826086Illumina Single End PCR Primer 1 (96% over 25bp)
GAATGATACGGCGACCACCGAAGATCTACACTTTTTTTTTCGTCGGCCTG21.7391304347826086No Hit
AGTTTAAAATGATCATTTCTCCCTTATTCATGTCCAGGTTAAATAATAAT21.7391304347826086No Hit
TATCAACGCAGAGTACTTTTTTTTTTCTTCTCCCCACCACCTTTTTTTTT21.7391304347826086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT10.8695652173913043No Hit
ATCAACGCAGAGTACATGGGCAGTCTACATCTTGCCTCAGATTCTGTCGG10.8695652173913043No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGAAGGT10.8695652173913043No Hit
AGATCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACTGT10.8695652173913043Illumina Single End PCR Primer 1 (100% over 25bp)
GCGTTGATACCACTGCCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT10.8695652173913043No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTAACATATGACGCTGC10.8695652173913043No Hit
ATCATTCTGTCTAGTTTCTATAGGAAGATATTTCCTATTCTATCATTGAC10.8695652173913043No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAAGT10.8695652173913043No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTCTTAT10.8695652173913043Clontech Universal Primer Mix Long (96% over 26bp)
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT10.8695652173913043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8695652173913043No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACCT10.8695652173913043No Hit
CTTTACTACTATTTGTGCTATATTTTTCACAATACTTCCTGTCTCTTATA10.8695652173913043No Hit
CAAACAGAGAGTCCTTTGACTTGGTCGAATCCAGCAGTCCTTCTCCATCC10.8695652173913043No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.8695652173913043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT10.8695652173913043No Hit
TATCAACGCAGAGTACATGGGCGAAAGCCCAAGGTTTCCTACGCAACGTT10.8695652173913043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.8695652173913043No Hit
TCCCAGCTATTTGGGAGGCTGAGGCAGGAGAATCACTTAAACCTGGGAGG10.8695652173913043No Hit
CCTTCTGTGAGCCCGTGCAGCTGCCGCGGGTGTTAGAACGGATTCTGTCT10.8695652173913043No Hit
GCCTGCAGGGTGGCCAATCTGATGTGTTGGGGAGCACAGCTGTCTCTTAT10.8695652173913043No Hit
CACGAGACATCTCAGGATCTCGTAGCCGTCTTCTGCTTGCCCATGTACTC10.8695652173913043No Hit
GTACATGGGGTGGTATCAACGCAAAGAAAAAAAAAAAAAAAAAAAAAAAA10.8695652173913043No Hit
TATCAACGCAGAGTACATGGGAAACCCTGTCTCTACTAAAAATACAAAAA10.8695652173913043No Hit
GGATATCTGTAATGTCAAAACCCCAAACTGCTCAGCAAAATTGGAATGGT10.8695652173913043No Hit
AGATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTCTGTCTCTTA10.8695652173913043No Hit
AAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG10.8695652173913043No Hit
ACTCTCCTACACGACGCTCTCCGATCTCATGTGCAAGGACTGGAGTTGCT10.8695652173913043No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAATGCTGTCTCTTA10.8695652173913043No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCATTTAAACCCAAAC10.8695652173913043No Hit
ATTCAGTGATGATTCCATTTGATTCCATCTGATGATGATTTCACTTGATT10.8695652173913043No Hit
TCACAAAGGAGTTTCTGAGAATCATTCTGTCTAGTTTCTATAGGAAGATA10.8695652173913043No Hit
AGCTGCATGGGTGCCTGCTGCTGCCTGCCTTGGCCTGATGGCCAGGCCAG10.8695652173913043No Hit
GAATTCCATTTGATGACAATTCCATTCAATACCAATTGATGATGGTTATT10.8695652173913043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAT10.8695652173913043No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATACCACAGCCCCAT10.8695652173913043No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAAAAAACCCC10.8695652173913043No Hit
AGAATGTCAAGGCAAAGATCCAAGATAAGGAAGGCATCCCTCCTGATCAG10.8695652173913043No Hit
GCACATACCTTGACAAATAATTAAAGTAAAATTTTACTTGTTTTTTTTCA10.8695652173913043No Hit
CTCCATAAGGTTTGCAATTTACCTCATCCAGATCCATCAGAGGAATAACC10.8695652173913043No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGGGTT10.8695652173913043No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTAAACAA10.8695652173913043No Hit
CTTCCGATCTATGTATTAACCGCCGTTAACATCCTATGAACCTGTCTCTT10.8695652173913043No Hit
TTTTTACACATCTGACGCTGCCGACGAAAAAAAAAGGTAGATCTCGGTGG10.8695652173913043No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTT10.8695652173913043No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10.8695652173913043No Hit
GCTCTCAAGTTTGCCAAACTACCACTTTCGGTTTCCATGTGTGTTTCTCC10.8695652173913043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAGGT10.8695652173913043No Hit
GATTTGTGGATCTTGAACCTACCCAAGCCTGGTTGTCTTCCTCTTCCAGC10.8695652173913043No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGG10.8695652173913043No Hit
AAAAATGGCTGATCAAGCAACCTATTTATTTTGCACCAGAACAAGTCGAG10.8695652173913043No Hit
AACGCAGAGTACATGGGCCACTACTCTTCCTACACTTGCCTCAGATTCTG10.8695652173913043No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA10.8695652173913043No Hit
CTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTC10.8695652173913043No Hit
GTCTCCAAACTGCTCAATCAAAAGAAAGTTTCAACTCTGTGAGATGAATG10.8695652173913043No Hit
ACGACGCTCTTCCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAGA10.8695652173913043No Hit
GTACATGGGGCAGTGAGCCAAGATCTCACCACTGCACTCCAGCCTGGGTG10.8695652173913043No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTAAAACACA10.8695652173913043No Hit
GGCGACCACCGAGATCTACACATTTTTTTCTGTCTCTTATACACATCTGA10.8695652173913043Illumina RNA PCR Primer (95% over 24bp)
AAATTCTGTAACAGGGCCCTTGAGCCCCTATGCTTGAGCCCGCTCCCACA10.8695652173913043No Hit
GGGGTTTAGAGTTTGCAAACAAATGCCCATTACATTCATTATGTGTTAAG10.8695652173913043No Hit
GTCTAGGAATTTGTCTATTTCTTCTAGATATTCCAATTTATTTGCATGTA10.8695652173913043No Hit
GTAAGGACCTATTCAAAGCATGATGGGGGAAATGTCACAGGCAAAAGTAG10.8695652173913043No Hit
GTAACTAATGAAGTAAGTTCTATGATTGCCCCCGGCTTACTAGATAGAGA10.8695652173913043No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAACAAAGCCCAATTA10.8695652173913043No Hit
GCGTTGATACCACTGCTTGTATCAACGCAGAGTACATGGGACGGCGACCA10.8695652173913043No Hit
CTTTGGTATAGACACCCTGGTGTCCTTTTAAGAGATGAGGATGTCGGCTG10.8695652173913043No Hit
GCCGCCGTCTGGACCGCGCTGGAGCCCACCCTGGGCAAACTCCTGTCTCT10.8695652173913043No Hit
TCCCTGGGGTGTGGTTCTCAGGCTCTTAGCACATGATGGTGGCTGGAACT10.8695652173913043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT10.8695652173913043No Hit
ATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTT10.8695652173913043No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAAACAA10.8695652173913043No Hit
GTGGGCCACCATCCTCCAGACCCCAGAATGCTAGATCCACTGACAGCTTG10.8695652173913043No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGA10.8695652173913043No Hit
TATGTGTATTGGAGCTGGGAAGTCGTGTCCGCCGCAGCCACCAGGTCGGG10.8695652173913043No Hit
GGTATCAACGCAGAGTACATGGGCCTGACTTGACTTCTGCTGAGATCGGA10.8695652173913043No Hit
GTTCCATTCCATTCCATTCCATTCGGATTGATTCTATTCAATTCCCTTAC10.8695652173913043No Hit
GACCAGCCTGGCCAACATGGCGAAACCCTGTCTCTACTAAAAATACAAAA10.8695652173913043No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAG10.8695652173913043No Hit
GGCATAGGCAGAACATAAGTGCTTGACACGGGCAGATTTGTTCATTCATT10.8695652173913043No Hit
GCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAACTGCTTTGATACC10.8695652173913043No Hit
GGAGATTGAGGTGGGAGGATGGCTTGGGCCCAGGAGGTCGAGGCTGCAGT10.8695652173913043No Hit
GCCCTACGACATGCTCACGGTGCTGCGCACGGTGCTCGACGACGAGGAGT10.8695652173913043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT10.8695652173913043No Hit
CTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCGCCGCCATGTACT10.8695652173913043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT10.8695652173913043No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCATTTA10.8695652173913043No Hit
GCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGT10.8695652173913043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10.8695652173913043No Hit
GAGCACACGTCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTT10.8695652173913043TruSeq Adapter, Index 7 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)