FastQCFastQC Report
Tue 10 May 2022
EGAF00003642828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078347
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT50670.46988585306955927No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC40470.37529663457124657No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT38880.36055184462886253No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC38570.3576770742627373No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA30460.28246937210378475No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT28970.26865192744079597No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG28820.2672609095217031No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA28640.26559168801879174No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG26010.24120250717069736No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT25100.23276366512820085No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA24070.22321200875043004No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG24010.22265560158279293No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT23920.22182099083133722No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA22690.210414643894776No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG21750.2016975982684609No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG21710.2013266601567028No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT21540.1997501731817309No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT21090.19557711942445244No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG21040.1951134467847548No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG20900.1938151633936015No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC20600.19103312755541585No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG20390.18908570246868586No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT20270.1879728881334116No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC19120.17730841742036654No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG17710.1642328489808939No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC17700.16414011445295437No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA17260.1600597952236154No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC16720.1550521307148812No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC16660.15449572354724406No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC16340.15152821865317936No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT16260.15078634242966318No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC16250.15069360790172365No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG15790.14642781961650564No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA15660.14522227075329186No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA15510.143831252834199No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA15460.1433675801945014No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG14820.13743257040637197No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA14500.13446506551230727No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG14490.13437233098436774No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG14100.13075568439472637No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC13830.12825185214035928No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT13760.1276027104447826No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC13570.1258407544139317No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT13320.12352239121544363No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT13100.12148223160077413No Hit
GTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATT13050.12101855896107652No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT12810.118792930290528No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC12710.11786558501113277No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA12390.11489808011706808No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG12350.11452714200530997No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT12330.11434167294943093No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC12190.11304338955827763No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG12180.11295065503033809No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC12060.11183784069506383No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG11940.11072502635978956No Hit
GGGCACCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC11830.10970494655245483No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA11590.10747931788190629No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG11390.10562462732311584No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC11360.10534642373929727No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT11270.10451181298784158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)