FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003642929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACGAGCACGGTCGCGCCCGGCGCGAGCTCCCCGCGCGTGAAGAGCATG210.526315789473683No Hit
CTATATGCCAGGTACTCTTCCAAGCCCTGGGCATGCAGCACTGAATAAGA210.526315789473683No Hit
GCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAG210.526315789473683No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15.263157894736842Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATGGAAGAGTCAATGAACTCGAATGGAATCATCATTGAATGGAATCGA15.263157894736842No Hit
ATCCACTGCCAGGCGCTCACGAGCACGATCACCCAGATCGCGAGCTGCGG15.263157894736842No Hit
GTGCTAGAGCACCACAAGAAGAAATTTGAACACAGAAAAATAAGGAAGCA15.263157894736842No Hit
GGGCTGAACCGCGCCTCGGCCAGCGCGATGAGCAGGAACGCGCCCGCCAC15.263157894736842No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTAGACCGTCGTGAGACAGGTTAG15.263157894736842No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15.263157894736842Clontech Universal Primer Mix Long (96% over 26bp)
AAGAGCTAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCC15.263157894736842No Hit
CGTCCATCCCCGGCATCGTCACGTCCAGCAGCACGACGTCGAAGCGCTGC15.263157894736842No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT15.263157894736842No Hit
GACTATATACGTTCTATAAAGGTGGAATGAGAGAATCAATGAATGGATGG15.263157894736842No Hit
CAGCGAGCACGAGCTCGTGACGGCCGACCCGACGTCGTCCGTGCTTCTCG15.263157894736842No Hit
TTCGTACTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)