FastQCFastQC Report
Tue 10 May 2022
EGAF00003642939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282456
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA5710.20215537995298383No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC5270.18657773246098508No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC4530.1603789616789872No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG4340.15365225026198773No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT3990.1412609397569887No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC3750.13276404112498938No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC3690.13063981646698955No Hit
GGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCAC3450.12214291783499023No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA3330.11789446851899056No Hit
GAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATC3290.11647831874699068No Hit
GCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCG3270.11577024386099073No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC3140.1111677571019911No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC3130.11081371965899114No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA3110.1101056447729912No Hit
CATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCG2970.10514912057099157No Hit
GAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCAT2880.10196278358399186No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT2870.10160874614099187No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC2850.10090067125499193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)