FastQCFastQC Report
Tue 10 May 2022
EGAF00003642940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282456
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC5830.20640382926898349No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC4720.16710567309598665No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC4680.16568952332398676No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG4500.15931684934998724No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA4350.15400628770498767No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC3870.13701249044098904No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT3760.13311807856798935No Hit
GAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATC3470.12285099272099018No Hit
GCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCG3390.1200186931769904No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC3370.11931061829099046No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA3360.11895658084799049No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC3340.11824850596199055No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC3310.11718639363299062No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC3170.11222986943099102No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC3160.11187583198799105No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC3140.1111677571019911No Hit
GGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCAC3080.10904353244399127No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA2950.10444104568499163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)