FastQCFastQC Report
Tue 10 May 2022
EGAF00003642953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31212
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA1730.5542739971805716No Hit
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT1430.45815711905677303No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG1220.3908753043701141No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG1130.3620402409329745No Hit
TCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAA1080.34602076124567477No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC1020.32679738562091504No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT900.28835063437139563No Hit
CCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCT880.2819428424964757No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG830.26592336280917594No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT810.25951557093425603No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC800.2563116749967961No Hit
GCTTTAATGGATCCATAAAGGGAAGAATGTTGTCCAGAGGTAAAGGTGCC770.24669998718441624No Hit
CTACAAAGGCTGATGGTCACCAGCAGGAAGATAGTTACCAGCTTCATGAC770.24669998718441624No Hit
GGTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACT680.2178649237472767No Hit
GGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTG670.21466102780981675No Hit
GTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAATGGATCCA640.20504933999743688No Hit
CTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCA630.20184544405997693No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA620.198641548122517No Hit
CATTAAAGCTTCTTCTGAAAACTCTGGGCATTTCTGTTGAGCACCTTGTG620.198641548122517No Hit
TAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTG610.19543765218505704No Hit
CATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGCACCTTTACCTCTGG610.19543765218505704No Hit
ATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATC590.18902986031013713No Hit
CCTTGTGGAGGGGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTT580.18582596437267718No Hit
ATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTC560.17941817249775727No Hit
CATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCC560.17941817249775727No Hit
GTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAAC550.1762142765602973No Hit
CCTGAAAAATATCCCAGATAACTGTCATGAAGCTGGTAACTATCTTCCTG540.17301038062283738No Hit
GGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCCCATCCACCTC540.17301038062283738No Hit
AAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGG530.16980648468537743No Hit
TATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGG520.16660258874791745No Hit
GCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGC510.16339869281045752No Hit
GAGGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTT510.16339869281045752No Hit
GGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAACTCCTGAAAAAT500.16019479687299756No Hit
CTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCT480.15378700499807768No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG470.1505831090606177No Hit
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA470.1505831090606177No Hit
CTTCCATCCCCATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGT460.14737921312315774No Hit
CTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTC450.14417531718569782No Hit
ATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAG450.14417531718569782No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG440.14097142124823786No Hit
CACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCT440.14097142124823786No Hit
CTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTACTGCC440.14097142124823786No Hit
GATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCAT420.13456362937331795No Hit
CCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTCCACAAGGTGC420.13456362937331795No Hit
CTTCAGAAGCCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTCC410.131359733435858No Hit
TTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAG400.12815583749839804No Hit
GTGCCCCTTCCTGTTGACAAGTTGGCACCTTTACCTCTGGACAACATTCT390.1249519415609381No Hit
CCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTC390.1249519415609381No Hit
GGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAAAACTC390.1249519415609381No Hit
CCATCAGCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTG380.12174804562347814No Hit
CCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAACTCCTGA380.12174804562347814No Hit
ACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGG380.12174804562347814No Hit
CTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGT370.1185441496860182No Hit
GTCCAGAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGCACTTTGTTGA370.1185441496860182No Hit
ATGTTGTCCAGAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGCACTTT360.11534025374855825No Hit
GGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTA360.11534025374855825No Hit
TTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACA360.11534025374855825No Hit
ACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAAAACTCTGGGC350.11213635781109829No Hit
TGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCC350.11213635781109829No Hit
CTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTG340.10893246187363835No Hit
CCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAA340.10893246187363835No Hit
GAAGAATGTTGTCCAGAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGC340.10893246187363835No Hit
GTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAA340.10893246187363835No Hit
TTTCTGTTGAGCACCTTGTGGAGGGGCTAAGGAAGTGTGTAAATGAGCTG330.1057285659361784No Hit
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG330.1057285659361784No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA320.10252466999871844No Hit
CTGTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGT320.10252466999871844No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)