FastQCFastQC Report
Tue 10 May 2022
EGAF00003642954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31212
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA2170.6952454184288095No Hit
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT1190.3812636165577342No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG1170.3748558246828143No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC1060.33961296937075486No Hit
CCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCT950.30437011405869535No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG930.29796232218377544No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT900.28835063437139563No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA890.2851467384339357No Hit
GCTTTAATGGATCCATAAAGGGAAGAATGTTGTCCAGAGGTAAAGGTGCC860.2755350506215558No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT850.27233115468409586No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG840.2691272587466359No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC710.22747661155965654No Hit
AAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGG680.2178649237472767No Hit
GGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTG660.2114571318723568No Hit
GCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGC640.20504933999743688No Hit
CTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTC620.198641548122517No Hit
TCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAA590.18902986031013713No Hit
CCTTGTGGAGGGGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTT580.18582596437267718No Hit
GGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTA580.18582596437267718No Hit
CATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCC570.18262206843521722No Hit
CTACAAAGGCTGATGGTCACCAGCAGGAAGATAGTTACCAGCTTCATGAC570.18262206843521722No Hit
GTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAAC560.17941817249775727No Hit
ATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTC560.17941817249775727No Hit
CCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAACTCCTGA540.17301038062283738No Hit
CTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCA540.17301038062283738No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG520.16660258874791745No Hit
ATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATC520.16660258874791745No Hit
TATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGG520.16660258874791745No Hit
CTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCT510.16339869281045752No Hit
CCTGAAAAATATCCCAGATAACTGTCATGAAGCTGGTAACTATCTTCCTG500.16019479687299756No Hit
CTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTG470.1505831090606177No Hit
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA470.1505831090606177No Hit
GGTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACT470.1505831090606177No Hit
CACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCT470.1505831090606177No Hit
CATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGCACCTTTACCTCTGG470.1505831090606177No Hit
GAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGCACTTTGTTGATGAGG460.14737921312315774No Hit
GATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCAT450.14417531718569782No Hit
CATTAAAGCTTCTTCTGAAAACTCTGGGCATTTCTGTTGAGCACCTTGTG450.14417531718569782No Hit
ATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAG440.14097142124823786No Hit
GTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAATGGATCCA440.14097142124823786No Hit
ACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGG430.1377675253107779No Hit
CTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTACTGCC430.1377675253107779No Hit
ATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAG430.1377675253107779No Hit
GAGGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTT420.13456362937331795No Hit
TAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTG410.131359733435858No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG410.131359733435858No Hit
GTTGACAAGTTGGCACCTTTACCTCTGGACAACATTCTTCCCTTTATGGA410.131359733435858No Hit
GGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCCCATCCACCTC410.131359733435858No Hit
ACAAAGGCTGATGGTCACCAGCAGGAAGATAGTTACCAGCTTCATGACAG400.12815583749839804No Hit
GTTTTCAGAAGAAGCTTTAATGGATCCATAAAGGGAAGAATGTTGTCCAG380.12174804562347814No Hit
GCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGCACCTTT370.1185441496860182No Hit
CTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGT360.11534025374855825No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA360.11534025374855825No Hit
GGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAACTCCTGAAAAAT360.11534025374855825No Hit
CTTCAGAAGCCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTCC350.11213635781109829No Hit
CCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTC350.11213635781109829No Hit
TTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACA350.11213635781109829No Hit
CAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAATGGATCCATAAAG350.11213635781109829No Hit
GAAGAATGTTGTCCAGAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGC340.10893246187363835No Hit
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT330.1057285659361784No Hit
TGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCC330.1057285659361784No Hit
GTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTGAAGAAACTGCTG320.10252466999871844No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG320.10252466999871844No Hit
GGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAAAACTC320.10252466999871844No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)