FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003642955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATAATCGACCGGCGGCGATGCAGGCGTCACGGGTTGCCGCACCCAG53.3112582781456954No Hit
CTCTTCCTGCGGCTCTACCCGGTGCTCACCAAGGCGGCCACCAGTGGCAT21.3245033112582782No Hit
CCATGGAAAGCCTGAAAGAGAGCTGTGCTTGCTGTGAGGATATTAGAGGA21.3245033112582782No Hit
GGTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCGGGCTCGG21.3245033112582782No Hit
CCCACTCCACTGACTTAAATGTTAATCTCCTTTGCCAGCACCCTCACAGA21.3245033112582782No Hit
AGAAGACAAAGTTGTCAGATGTTTGCATGCAAACGTGGAATCACCAGGAT21.3245033112582782No Hit
CTGCGGGAAGCCTGCTGCCATCACCGCCTGAGCGTGATGCGCGGTATCAA21.3245033112582782No Hit
CCTGGGACTCCAGTCTTCATGTGTCAAGTTCTGTGCTTGTTCTGGAGAAT21.3245033112582782No Hit
TTTCTGCTCTTGTGCCAATTTTTTCTCAACACTGCAGTCAAATTGCGTAC21.3245033112582782No Hit
ACTCAGGTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCT21.3245033112582782No Hit
CAAAAAAAGGGCAGAACACTCTGCATTCATGAAAAGAGTCGTGTAGCACA21.3245033112582782No Hit
TTGTGAACCATATTTCAATTTATTGACATTGTCAATTTATGAACAAGACA21.3245033112582782No Hit
AGCCTATCTAGCTAGTTAAGGTTGTTCTCTTCTGCCATACGTAGATATTC21.3245033112582782No Hit
ACGCAGAGTACATGGGACAGCGGAACTAAGAAAAGAAGAGGCCTGTGGAC21.3245033112582782No Hit
GTATCAACGCAGAGTACATGGGACGGCGCTGGGGAGGCGATGGCGCCGGC21.3245033112582782No Hit
AACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTG21.3245033112582782No Hit
GTCCTCTTGCTCCTGACGCTTGAGCCATCCTGTAATCCAGTATGTGACAG21.3245033112582782No Hit
GAGTAGCTCGCTGGAGGCGGTGCGCAGGAAGATCCGGAGCCTGCAGGAGC21.3245033112582782No Hit
GGATGATAAAGACACGATCGAGAAACTGAAGGAGAAGAAGCTGACTCCCA21.3245033112582782No Hit
GTCATAGGATGTGATAATTTCCCTGGAAATCAGAGGGGAAAAGAAGCAAA21.3245033112582782No Hit
ATACAATGAAATCTACAAAGACACACTTTTTAACTTCAAGCGTTGTTGAT21.3245033112582782No Hit
GTACCTGACCCTTATGTATTTCTATCCTGATAGCTCTATCTGCCTAAAAG21.3245033112582782No Hit
CTTATGATGAAGAATTCTGTGAAGCTCTATTTCTTTGTCAATCTTTTCCC21.3245033112582782No Hit
ACCCTAATCTGTGTGCTTTATTCGTTTGTCTTCTTTATGGATCAGGTGCT10.6622516556291391No Hit
CAATGGTGCGATCTCCACTCACTGCAATTTCTGCTTCCTGGATTTAAGCA10.6622516556291391No Hit
GTGTAGGACCACACTATTTGTTCTTTCATAGACCCGGCTAGGATCAGCTT10.6622516556291391No Hit
ATTCCCATTTCACATAAATGGAAACCGGGGCACGGAAAGGTTATCAGCTT10.6622516556291391No Hit
TTCCAGAAGTAGAGAGAGAGACAGAAGCCGAGAGCGAAGAAGATCTCGAA10.6622516556291391No Hit
CAGAGGCGGCGGCGGCTCAGGGGAAACGAGGCTGCAGTGGTGGTAGTAGG10.6622516556291391No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6622516556291391No Hit
ATGGAATATAAAGAAATGGAATCGAAGAGAATGGAAACAAATGGAATGGA10.6622516556291391No Hit
TCTGTGTATTTGTCAATTTTCTTCTCCACGTTCTTCTCGGCCTGTTTCCG10.6622516556291391No Hit
CTTCAGGCACTCTTCACAGGTTTTGTTTGTGTTCTGAGAACAAGCAGCTC10.6622516556291391No Hit
ATCATAGACTTCCCCTATTTTGATGTTCAGTGTCTGCCTGCACATACATA10.6622516556291391No Hit
TCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATT10.6622516556291391No Hit
GGGCAATGTGGCGAAACCCTGTCTCTACCAAAAATACAAAAAATTAGCTG10.6622516556291391No Hit
CCCTGTCAGCCCGTTCTTGCTTCCCTCCTAACCGAAGATTCCACAGTCGA10.6622516556291391No Hit
AGCTGGGACCACAGCCATGCACCACCATGCCCAGCTAATTTTTGTATTTT10.6622516556291391No Hit
GGTGTGTGGCCCACCAGCACGCGCCGGCGCTCCGGCAGCAGCTGCGCCCG10.6622516556291391No Hit
CCTCACTCCTGTCCGGCTTCCGGCTCCTCTTCCTCCTGCAGCAGCAGCAG10.6622516556291391No Hit
AGCTAATTTTATTCTTTCCCAACAGGATCCATTTGGAAAATATCAAGCCT10.6622516556291391No Hit
ATACAACATTACTGACTTACGTGATGGGAACTGTACAAGGCAGAGGCTCC10.6622516556291391No Hit
TTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTT10.6622516556291391No Hit
TTTCCCCACTTACCCATTGGCTAGTAAAACCCAATGATTTGTAGCACCAT10.6622516556291391No Hit
GACACACGGACACAATCAGCCGAGAAGTTCCTGGTCTGAATCACGAGAAT10.6622516556291391No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACGGGTTTATAACCGTT10.6622516556291391No Hit
CCTCCCGCTCACGCATGGCCTTCTCCTCACTCCTGTCCGGCTTCCGGCTC10.6622516556291391No Hit
GCATATTAAGGGTCCTTGGTTTTTGACAGTCACCTATACTGTTAGAAATG10.6622516556291391No Hit
TCCTGACCGACTATCGGCTGGACACCGCCACCATCATCACCGCCCTGCTG10.6622516556291391No Hit
GTCTTCTGCCATTCAGGTCATAGCCTGCAGTGCTACAACTGTCCTAACCC10.6622516556291391No Hit
ATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCC10.6622516556291391No Hit
CCGCTGAAGCCGACGTAGCTTCTCTGAACAGACGTATCCAGCTGGTTGAG10.6622516556291391No Hit
AATTTTTCCTTTCCATATTTAGTGCTTCCTTCAGGAACGCTTACAAGGCA10.6622516556291391No Hit
TTTTTTCCCATGGAATGAGATCCTTTTCAATCTGCCATAACATGTGCCCA10.6622516556291391No Hit
ATATCACGGCAGGTCATTTCTTTCATCTGAAGCTTCTCTATTTCCGTCTT10.6622516556291391No Hit
CGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTG10.6622516556291391No Hit
ACACAAAGAAGTTACTAAGAATTCTTCCCTCTAGCATCTGTCTCTTATAC10.6622516556291391No Hit
TTTTTTTCCTTACTAAATTCTCAGCATGCTGGCTGCTGGTCCCATCTCTT10.6622516556291391No Hit
GGCGTCCACATTAAAACCAAGGGCAGCCGGGCGCAGTGGCTCACCCCTGT10.6622516556291391No Hit
GTCCAGTTCCTTTTTTGTTCTTCTTACTCAACTCCGGTGGACGCCCTAGG10.6622516556291391No Hit
GGAAGAGACGCCCCTGCTAACGGGAATGCTGAGAATGAGGAAAATGGGGA10.6622516556291391No Hit
GGGCTATGGGCCCATCGACTACAAACGGAACTTGCCGCGTCGAGGACTGT10.6622516556291391No Hit
NAAATGGGTGGGAGTGGCGAATTGGACTTTGCCTTCATTTTAAATTCATG10.6622516556291391No Hit
CGCGTGAAGGCCGGGCTGGCCTCGCCGCTCGAATACTGGATGTACGAGCG10.6622516556291391No Hit
CTAGAACAGGATGAACACAAGAGTCCAACTGGGAAAGACGTTCCAACAGA10.6622516556291391No Hit
GTAGGTTGTCTGTTCACTCTGAGGATAGTTTCTTTTGCTGTGCAGAAGCT10.6622516556291391No Hit
GTCTCGGAGCCGGAGCTCGTCCCGCTCCAAGCACACCAAGAGCAGCAAGC10.6622516556291391No Hit
GGTTCTTTGAATGATTGGTTGGCATCAATTAACAATAGGTAAATGTTCCT10.6622516556291391No Hit
GGGTACCACCACAGTTGCTATCTCTTGAACATCTTTCATTAAAACATCAC10.6622516556291391No Hit
GAATGGACTCAAATGGAATCTTCATTGAATGGAATCGAATGTAATCGTCG10.6622516556291391No Hit
CATTAATGCATGAGGAAAATAAGATCCAGACGTGTTAATTGACTTACCTA10.6622516556291391No Hit
ACCAAGGGAGGGCCAGACTCATTGGCAGATCTCAGTTTCCACAACATCCC10.6622516556291391No Hit
GGCCTGGGCAGGCACGGCCCACGGCGCCAGGAGCGCCATCGCCAGCACCT10.6622516556291391No Hit
GGCGGCGGGAGTCGCGGTCCCGTTCGCGCTCCACCAACACGGCCGTGTCC10.6622516556291391No Hit
CCATATCTTTGATCACTCCATTTATAGACTTGTGCAAACACTGAGGTGGT10.6622516556291391No Hit
CTCCACATCATCCAACATCCTCCTGGCCTCTGTTGGGTCAGTGTTGGGGG10.6622516556291391No Hit
CACCATCTATTTTCCACAGGCCAACATCACCAGGTAATGCACTTTTTAAC10.6622516556291391No Hit
GTATTCACCTTATCCTCCCAACCCTCACCCCTGGTCTGTGTTGTGTGTGT10.6622516556291391No Hit
GCCCATGAGAGAAAGCAGTGGAAGAAGAGTCTCTGCATGACCAAACTGGA10.6622516556291391No Hit
GTGCAGGGTTCAGCAGTTATGTGGGAGTGCTAGGGGTTAGGCTTTTGAGC10.6622516556291391No Hit
TGCCTGTAATCCCAGCTACTTGGCAGGCTGAGGCAGGAGAATCGTTTGAA10.6622516556291391No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT10.6622516556291391No Hit
TTACTGTTTAACAGACTTGTTTGATTCCTTTTGTGTTGTTTTATGAACTG10.6622516556291391No Hit
GGGGATCAAAGGTGAAGGGTCACACATTATTAAAAAGAAATCCAAAAGGA10.6622516556291391No Hit
CTCCTACTCAGTTTACTCTTGCTTGTGTGCCTTTGCACACTCTCTTCTCC10.6622516556291391No Hit
TACTGGTTCTGGGCAATTATCCTGCAAATTCTGCCAGGTAATAAAAGTGA10.6622516556291391No Hit
TATCAACGCAGAGTACATGGGGCCCTGGCCCGGCCTTGCCTTGCGCTGCG10.6622516556291391No Hit
GTCTTGTTCTGTCCCCTAGGCTGGAGTGCAGTGGTGCAGTGTCGGCTCAC10.6622516556291391No Hit
AGCGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCT10.6622516556291391No Hit
GGTGTGACCCCATATATGATTTTCTTTAAGGATGGTTTAGAAATGGAAAA10.6622516556291391No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACAAATATGA10.6622516556291391No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG10.6622516556291391No Hit
CTTCCAGGTAGTGCAGAAAGAGTGTTTCAAACCTGCTCTATGAAAGGAAG10.6622516556291391No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTCA10.6622516556291391No Hit
GTGCTTAATAATATCCAGAACAGAACCTCCACTTAGCAGCTTATTGTGCC10.6622516556291391No Hit
ATCTTTCTCTCTGTGCCTTCAGTTTTGGATGTTTCTGTTGGCATATCCTC10.6622516556291391No Hit
GGCTGGTGATGACCGAGGCGGTCGACGGGCCGGTGGCGTTCGGATTGTGG10.6622516556291391No Hit
CCCGTCGTCGAGGGCATGGGCTGGGTGCGCTCGGATGAGGGGCGCTCGAG10.6622516556291391No Hit
CCTCGTGATCCACCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCAT10.6622516556291391No Hit
CGCCTCCGCCGCGCGCCTCCTCCGCCGCCGCGGACGCAGGCAGCATTATA10.6622516556291391No Hit
GCCTATCACGTTTCTTTAAAAAATCTAAGAAGGAGGAACAAAACTAATGG10.6622516556291391No Hit
CTGTTGCCTGCAGACATAAATTGCTAGGGAACCTTAAATGCCTGACGATA10.6622516556291391No Hit
GCCTGGTGCAGACCCCCAATTCCTACTTCATGGATGTGAAATGCCCAGGA10.6622516556291391No Hit
ATATGTAGCGGCGTCAGCCCTGTTCCGTTTTTGTGTGACGCTGGTCAGTA10.6622516556291391No Hit
GATCTACACTTTTTTTTTCGTCGGCAGCGTCAGATGTGTAAAAAAAAAAA10.6622516556291391No Hit
CAATAAAGAAAAGCTGAACGTTGGCTGGGCACAGTGGCTCACACCTATAA10.6622516556291391No Hit
GTGTTGGACTCAGGGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCA10.6622516556291391No Hit
AACATATAGATCATTTATTTTCCTTCTAGTCCCGGGTTCAGTACATAGAT10.6622516556291391No Hit
GTCTCGCTCTGTTGGCCAGTCTGGAGTGCAGTGGCACAATCTAGGCTAAC10.6622516556291391No Hit
AGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGC10.6622516556291391No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC10.6622516556291391No Hit
CATCAAGAAGATCTTGGACAGCGTGGGTATCGAGGCGGACGACGACCGGC10.6622516556291391No Hit
GCATGTCTTTGCGCAATATCGCCATCATCGCGCACGTCGATCATGGCAAG10.6622516556291391No Hit
CCCGGGTTCAAGCGATTCTCCTGCCTCAGTCTTCAGAGTAGCTGGGATTA10.6622516556291391No Hit
CGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCG10.6622516556291391No Hit
CACTCGGCCATCGTCATGCACGTACTTGTAGCTGTAAACCACGAACCTGG10.6622516556291391No Hit
CTCTTGGGGAGACGCGGGCCCTCTTGAGTCACTGAACTTGAGCTATTTGG10.6622516556291391No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTA10.6622516556291391No Hit
TACCGGGTAAGCGCTAGCAGCTATAAATAGCATAGCGGTGTCTGTTTGCG10.6622516556291391No Hit
ACCTCCGACAGCGGGATCGACTTGTCGCCGGACACGGCGCGTCCTTCGAT10.6622516556291391No Hit
CACCACCATCTGCCGGTCTTTGTCCACCTTCATTATGATGGCTGCATTGT10.6622516556291391No Hit
ACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTGAAAGAAGCGGA10.6622516556291391No Hit
GTGCAGAAATGGCACCTCGAAAGGGGAAGGAAAAGAAGGAAGAACAGGTC10.6622516556291391No Hit
ATCAAAAATCATCTCGTCCCCAGACATGGCTGCGGCTCGAGTGGGCTCGG10.6622516556291391No Hit
GTTTATGTGCCAGGTTCTGTAATAGGTACAGCAAATAAAGGGGTGAATAA10.6622516556291391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)