FastQCFastQC Report
Tue 10 May 2022
EGAF00003642971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12753
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCC570.44695365796283226No Hit
GTACATGGGAGCGTGGTCCAGAATGGATTCTTTGCCCATAAAGTGGAGCA330.258762644083745No Hit
CCCTCAGAGAGTCCACTTCACTCCCCTGCTACCTCCACCAGAGCAGGTGC310.24308005959382106No Hit
GGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCCCTGGCAA280.21955618285893516No Hit
GTACATGGGAGTTCCTGTGCTGGAGAGAAAGGCAGTCAGAGGAGAAAATG280.21955618285893516No Hit
GAGTATGTCAGAGGAAAGATCTGGGACTCAAGGGCTGCTGCTCAGGTTCT260.20387359836901123No Hit
GGGTAGAGTATGTCAGAGGAAAGATCTGGGACTCAAGGGCTGCTGCTCAG260.20387359836901123No Hit
GTTTAGTACACTGCCTTTGTGTTGGTTGGCCTCCAGCCAGGAGGTGGCGC260.20387359836901123No Hit
GCTTAAAACATGTCTCTGCATTTTATTTTAAGAAAACACAAACCTGGTCA240.18819101387908727No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG220.17250842938916333No Hit
GTCCTATAGGGAAGATGCAGACTTGCCCTAGGGCAAGTATTCAGGTTTCC220.17250842938916333No Hit
GGCTTGTACTGTGGAGTGTCTGCAAAGTGTCCTGTGATGTGATCCATCTT190.14898455265427743No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA180.14114326040931546Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAGCGTGGTCCAGAATGGATTCTTTGCCC180.14114326040931546No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT180.14114326040931546No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT180.14114326040931546Clontech Universal Primer Mix Long (96% over 26bp)
ATTTATGACTCAGCAGAGGCAGCCATAATCCCCCGGGAACATAATTCCAC180.14114326040931546No Hit
GTACAAGCCTGGATCCCAGTAGCTCAGCTGGGTGGCTAGACCCAGAGGAG180.14114326040931546No Hit
GTGTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGTTTTTCTGGTTC180.14114326040931546No Hit
GATCTATTGTTAGTGAGCTGGTATGATATTTTGGTGGTGTTAAAGGACCT170.13330196816435347No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG170.13330196816435347No Hit
CCCTTGGTGTGGCGTTCTCCCCTTTCCCTTAGGAATGGGGCTTCCTGAGA170.13330196816435347No Hit
TCCCAGGAGTGGGCAGGGCCGTAGAGATCCCAGGAGATTATGATCTTTGT160.1254606759193915No Hit
CTCTACGGCCCTGCCCACTCCTGGGAAACCTGAATACTTGCCCTAGGGCA160.1254606759193915No Hit
GTTTTATCGTATTACCAGAATTGTTTTTCTGGTTCTTTCTCATTTGGGTA160.1254606759193915No Hit
TCTTTATGTAGTTTTCAAAGTCACTTCCGAAAAAGAAGATGAGGTAATAG160.1254606759193915No Hit
CTATAGGACAGCACCTGATGTACTCTTGAAAGCGCCACCTCCTGGCTGGA160.1254606759193915No Hit
CCCTATTTCTGCCCTCACCTGGTGGTCTTTCTCTGCCTGCCCTGGTAGCC160.1254606759193915No Hit
ATATTTTGGTGGTGTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGT160.1254606759193915No Hit
GTATTTGTTTGTTTAGTACACTGCCTTTGTGTTGGTTGGCCTCCAGCCAG150.11761938367442955No Hit
GTCCAGAATGGATTCTTTGCCCATAAAGTGGAGCACGAAAGCAGGACCCA150.11761938367442955No Hit
ACACAAACCTGGTCACAAAACATCTTCAGAGAACAGGATAAGTGAAGAAA150.11761938367442955No Hit
GAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCCCTGGCAAC150.11761938367442955No Hit
ATCTTACTCTGCTCCAAGGCCACAGACCACATCCCCAAATCTGATACACA150.11761938367442955No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT150.11761938367442955No Hit
CTCTGAGGGTTATTGGTTATGTATTTGTTTGTTTAGTACACTGCCTTTGT140.10977809142946758No Hit
GCCTTTGTGTTGGTTGGCCTCCAGCCAGGAGGTGGCGCTTTCAAGAGTAC140.10977809142946758No Hit
GTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGTTTTTCTGGTTCTT140.10977809142946758No Hit
GTCACTGGGAGGTGAGTTTATTCAGGGCAGGGATCTTGTTTTATTCTCCT140.10977809142946758No Hit
GTATAGAATTTGTCACAGTGGAACGTAATTGTCACTGGGAGGTGAGTTTA140.10977809142946758No Hit
GAACAGACTTCCATCTATAGCTGAAAGACGTCAGAAGGTTGTTGCTGCTG140.10977809142946758No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA130.10193679918450561No Hit
AGGAGAGACTGAGCTCAGACACCCTATTTCTGCCCTCACCTGGTGGTCTT130.10193679918450561No Hit
GTGTTAGCCAGGATGGGCTCGACCTCCTGACCTCGTGATCTGCCCGCCTT130.10193679918450561No Hit
GCCCTAGGGCAAGTATTCAGGTTTCCCAGGAGTGGGCAGGGCCGTAGAGA130.10193679918450561No Hit
GTACATGGGAGGCTAAATGCAAAGTCATCTCTTGAGTCATGAGCTGGACT130.10193679918450561No Hit
GTACATGGGAGAAGTGGGCAGCTGTGGTGGTGCCTTCTGGAGAAGAGCAG130.10193679918450561No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG130.10193679918450561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)