FastQCFastQC Report
Tue 10 May 2022
EGAF00003642972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003642972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12753
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAGCGTGGTCCAGAATGGATTCTTTGCCCATAAAGTGGAGCA440.34501685877832666No Hit
GTACATGGGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCC440.34501685877832666No Hit
CCCTCAGAGAGTCCACTTCACTCCCCTGCTACCTCCACCAGAGCAGGTGC350.274445228573669No Hit
CTCTGAGGGTTATTGGTTATGTATTTGTTTGTTTAGTACACTGCCTTTGT290.22739747510389713No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG290.22739747510389713No Hit
GCTTAAAACATGTCTCTGCATTTTATTTTAAGAAAACACAAACCTGGTCA280.21955618285893516No Hit
GCCTTTGTGTTGGTTGGCCTCCAGCCAGGAGGTGGCGCTTTCAAGAGTAC250.19603230612404926No Hit
GTACATGGGAGTTCCTGTGCTGGAGAGAAAGGCAGTCAGAGGAGAAAATG240.18819101387908727No Hit
GGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCCCTGGCAA230.1803497216341253No Hit
ATTTATGACTCAGCAGAGGCAGCCATAATCCCCCGGGAACATAATTCCAC230.1803497216341253No Hit
GTGTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGTTTTTCTGGTTC230.1803497216341253No Hit
CTATAGGACAGCACCTGATGTACTCTTGAAAGCGCCACCTCCTGGCTGGA220.17250842938916333No Hit
GGTCTACACTGCCAACCAGAACTGTGTAGATGCGTACCCCACTTTCCTCG210.16466713714420136No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA200.1568258448992394No Hit
ATATTTTGGTGGTGTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGT200.1568258448992394No Hit
GGGTAGAGTATGTCAGAGGAAAGATCTGGGACTCAAGGGCTGCTGCTCAG200.1568258448992394No Hit
GTACTAAACAAACAAATACATAACCAATAACCCTCAGAGAGTCCACTTCA200.1568258448992394No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT200.1568258448992394No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT190.14898455265427743Clontech Universal Primer Mix Long (96% over 26bp)
GTACAAGCCTGGATCCCAGTAGCTCAGCTGGGTGGCTAGACCCAGAGGAG190.14898455265427743No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG170.13330196816435347No Hit
GAGTATGTCAGAGGAAAGATCTGGGACTCAAGGGCTGCTGCTCAGGTTCT170.13330196816435347No Hit
GTCCAGAATGGATTCTTTGCCCATAAAGTGGAGCACGAAAGCAGGACCCA170.13330196816435347No Hit
GTATAGAATTTGTCACAGTGGAACGTAATTGTCACTGGGAGGTGAGTTTA170.13330196816435347No Hit
GGTATGATATTTTGGTGGTGTTAAAGGACCTTGTTTTATCGTATTACCAG170.13330196816435347No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC160.1254606759193915No Hit
TTTCAATCCTGTTTCATGTCTTGCCCAGGGGTTGGGTACTTTGGGTTTCT160.1254606759193915No Hit
GTACATGGGAGAAGTGGGCAGCTGTGGTGGTGCCTTCTGGAGAAGAGCAG160.1254606759193915No Hit
ACGTAATTGTCACTGGGAGGTGAGTTTATTCAGGGCAGGGATCTTGTTTT160.1254606759193915No Hit
GTTTAGTACACTGCCTTTGTGTTGGTTGGCCTCCAGCCAGGAGGTGGCGC160.1254606759193915No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA150.11761938367442955Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAGTGGGGTGAGGCCTGTGACTGCTGGCT150.11761938367442955No Hit
GCCCTAGGGCAAGTATTCAGGTTTCCCAGGAGTGGGCAGGGCCGTAGAGA150.11761938367442955No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG150.11761938367442955No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT140.10977809142946758No Hit
GGACTGGGAACGACATCGCCATCCCCCCACAGCAGCCACAGCAAGCCCCA140.10977809142946758No Hit
ATCTTACTCTGCTCCAAGGCCACAGACCACATCCCCAAATCTGATACACA140.10977809142946758No Hit
ACGCAGAGTACATGGGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTC130.10193679918450561No Hit
GATTAGATGAGAACACCAACCCCATATTCAGCAGAGAGTTAGGGAATGAG130.10193679918450561No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC130.10193679918450561No Hit
ACCTAGGAGAGAGAACGCAGAGCACCCCTGGCTACATATTTGGGAAACGC130.10193679918450561No Hit
GCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGC130.10193679918450561No Hit
ACACAAACCTGGTCACAAAACATCTTCAGAGAACAGGATAAGTGAAGAAA130.10193679918450561No Hit
GAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCCCTGGCAAC130.10193679918450561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)