FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT60.482315112540193No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGAT50.40192926045016075No Hit
TTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTT40.3215434083601286No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA40.3215434083601286No Hit
GGCAAACTCAACCACCCCTTCATTTAATTTAGGTCGGTGTGCATCCGCAA40.3215434083601286No Hit
GTGCAGCGCCGCCCAGGCCGCCTGGCGGGAGAACTTCCCCCTGTGCGGTC30.2411575562700965No Hit
AATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGTTCAACC30.2411575562700965No Hit
TCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGC30.2411575562700965No Hit
CTCCATTCCAGAACCATGGCCCAATTTGTCCGTAACCTTGTGGAGAAGAC30.2411575562700965No Hit
GGCCAAGGGGCTGAAGAAGATGCAGAGCAGCCTGAAGCTGGTGGACTGTA30.2411575562700965No Hit
ATCTATCCCAGCTTCTGTTCCAACCATTAAAATGGATCAGATATTCTGGG30.2411575562700965No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA30.2411575562700965No Hit
CCTCATAAGTGTCGTCATCAGTGGGCAGCTCATAGCGGCACAAGGGACAG30.2411575562700965No Hit
GCACTAGACAGAAGCAACAGACTCAATAGCCGGGTATGGTGGCACATGCC30.2411575562700965No Hit
TCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCT30.2411575562700965No Hit
GGTTGGCCTGGTCGAAGGTGATGCACACCTCTGCAACACTTCCAGGCTGG30.2411575562700965No Hit
AAACAGAGGGTTGCGGCCTGAAAGTGGGATCCGGGCACTGTCTCTTATAC30.2411575562700965No Hit
GCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCC30.2411575562700965No Hit
AAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCT30.2411575562700965No Hit
GAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGT30.2411575562700965No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG30.2411575562700965No Hit
GAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCC30.2411575562700965No Hit
TCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGGGCTGTGCCAAGTG30.2411575562700965No Hit
ATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGA30.2411575562700965No Hit
GTACGGGCCCTTGTGCTCCAGCTGTCTCCACTTCACCCCGTCTTCGTGCT20.1607717041800643No Hit
GTCTCCTACTCCTCAATTAAGTAAATGAGAATGATTCAGCCAACAAAGTT20.1607717041800643No Hit
GGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATA20.1607717041800643No Hit
GGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTG20.1607717041800643No Hit
GAATACGGAAGAAAGAAAAGGACACTGATTCTACAGGTTCACCAGATAGA20.1607717041800643No Hit
GCTGTCTCTCCACCTCCTCACATTATGCTAACAGGGACGCAGACACATTC20.1607717041800643No Hit
AAGGCAGACTGAGCGCCAGAGAGGACGTTTCCAACTCAAAAATGCAGGCT20.1607717041800643No Hit
GTACATGGGACGGTGCCGCCATGAGATTGACCCCGCGCGCGCTGTGCAGC20.1607717041800643No Hit
TTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTG20.1607717041800643No Hit
ATATACTACTTTTTCAATATATTCTGCCTCCCCGGAGTTTTGGGCAGCTT20.1607717041800643No Hit
GTCTATCCCTTGAGCGACTTCTACTTCTACGTCTCTCTCGGGAAGGACTC20.1607717041800643No Hit
CTGCACTCCAGCCTGGGCGAGAGAGCAAGACTCAGTCTCAAAAAAAAAAA20.1607717041800643No Hit
GTATCAACGCAGAGTACATGGGACTGGCTGCTCTGAAAAGCCATCTTTGC20.1607717041800643No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC20.1607717041800643No Hit
GAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTT20.1607717041800643No Hit
CCACAGGAATAAAAAACACTGGGAAGGGGTAACCCCCTCACCCCCGGGAG20.1607717041800643No Hit
CAAGGATGGTGGCGACCGCAGCAAGTCGAGGAGTCGGGGCAAAGCTGGGC20.1607717041800643No Hit
AGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAAT20.1607717041800643No Hit
GCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTT20.1607717041800643No Hit
TTTCTACGGTATGACTTTCGTTGTCGAATGACCACTGCTGGATGTACCTT20.1607717041800643No Hit
ACCTCAATGTAATTTTAATGACCTCAGACCAGTGACAAGGGCAGACTGAC20.1607717041800643No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA20.1607717041800643No Hit
CCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAAAAGCGTTG20.1607717041800643No Hit
AGGAGGAGAAGCGGCGCGGCCTGGACAAGCGGACCCCGGCCCAGGCGGCC20.1607717041800643No Hit
GCACCATCCTGCACCTTGTTGTCATGAACTTTGTTGGCTGAATCATTCTC20.1607717041800643No Hit
ATGTACAATATGTGTCCATGAGAAAAGCTCTAAAAGTGAAGAGACCTCGT20.1607717041800643No Hit
CAGCTGTCCTGGCATCAGGCACAGCAGCTGCACTTGTCTGACGTCCCTTT20.1607717041800643No Hit
ACATGAGAGAGATATAATGCTTAAAAGCCAAGCTAATGGTTCAAAAGAGA20.1607717041800643No Hit
AAAGAAAAGAAAAAAGAAAAAGAGAAGAAAGAGGGAAGAAAGAAAAGAGG20.1607717041800643No Hit
GTCCCGAACCCCTGGTAATAGAATTGTTTACCTTTATACCAAGAAGGTTG20.1607717041800643No Hit
TTCTTAGGCCGCAGGTCCAGGGGCTTGTACTTCTTGCCCTTGTAGAATTT20.1607717041800643No Hit
CACCTGCAGCGTCCAAGGCTTCCTGAAAAGCAGTCTTGCTCTCGATCTGC20.1607717041800643No Hit
GTCATCTCCCTGGGCCTCCAGCTCAGGATCTTTCACTTCAAATTCTTTCT20.1607717041800643No Hit
GAATATCACCAACAGCATGACCTTTGCGACCAAATCCAGCAACCAGAACT20.1607717041800643No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGA20.1607717041800643No Hit
CTTCCTCCCCACCTTCTTCCTCTTCTTCGTCTACCTCATTGTCAGCCTCC20.1607717041800643No Hit
CCTGCAGCATTCTCTTTGGTTTAGCTTAGGTAGTAAATAGCTTGTGTGTG20.1607717041800643No Hit
GTGTGGCCCAGCCCGAGGACACCGTGCAGTTCCGGATCCCCATGGAAATG20.1607717041800643No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG20.1607717041800643No Hit
GGTAGAGCCGGGTTCCTCGGGTCTGATGCTGTAGCCTTCTTCATCCAGCT20.1607717041800643No Hit
TCTCTAAATAGGAAATAATCCTTGTCATCTAGAACTACTTTGGTGCCTCC20.1607717041800643No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT20.1607717041800643No Hit
AATCCCCTCTGCACACAGACAGGGAATAAAAGTTTGACAAACACCCAAGA20.1607717041800643No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGATGCGTGC20.1607717041800643No Hit
GCCCTCTGGGGCTCAGCCCTGAGCTGGCGTCACCTGTGCAGGGCCCTCTG20.1607717041800643No Hit
TTCCAGACCAGTGTTGTCTCCCGGGACATTGACACAGCAGCCAAGTTTAT20.1607717041800643No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.1607717041800643Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TTCTTTATCAGGTTCCTGTTCCCCTGCTGCAGAAGGCCCTTTGCTCTTCT20.1607717041800643No Hit
CCTCAGGCGAGCTACAGCTTTTCTTGCCAATTTACGCTGTAATCTACGAT20.1607717041800643No Hit
GGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCT20.1607717041800643No Hit
GCCCGCGCCCCGAGCCCGCCGCCGCCCTTCGAGGGCGCCCCAGGCCGCGC20.1607717041800643No Hit
GTATCAACGCAGAGTACATGGGGGCCCTGAAGCGCGTACACCTGTCTCTT20.1607717041800643No Hit
GGCTGTGCACTGCATCAGGGGCTCCGGACCTCCGAGAACCGCAGCGGGGG20.1607717041800643No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT20.1607717041800643No Hit
GTTCCCACTGCGTGACCGAGCCAGCTCGCACCGCATCCATCCGCGAGCGG20.1607717041800643No Hit
AACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAA20.1607717041800643No Hit
ATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGG20.1607717041800643No Hit
CAAAAGGGACCCGCTGCAGACTCAGGGCAAAGGGAATGCCATCGGTGCTG20.1607717041800643No Hit
CATCAAGAAGAACGGCCAATCCGACAGATTTTGTACCTCGGGGACCTGCT20.1607717041800643No Hit
GAAAAGCCATCTTTGCATTGTTCCTCATCCGCCTCCTTGCTCGCCGCAGC20.1607717041800643No Hit
TCACTAACGTGGTGAAAACAACTGTTCTTCTGGCTGACATAAATGACTTC20.1607717041800643No Hit
GGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCC20.1607717041800643No Hit
CAACCCACCAGAAGAAGCACCAAGAAAACCTGGCATATTTCCTAAAACAG20.1607717041800643No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA20.1607717041800643No Hit
GAAATTGACCAACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTAT20.1607717041800643No Hit
GTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCTTCTCCTTGCC20.1607717041800643No Hit
AGGAAGTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTCTTCAAAT20.1607717041800643No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT20.1607717041800643No Hit
GCTTTCCAACTGCCTGACTGCTTGTTCGTCTCACTGGTGTGAGCTCCAGC20.1607717041800643No Hit
CTGTTGCCTCCGCTGGCGCTGCTGCTGCTTCTCGCGGCGCTTGTGGCCCC20.1607717041800643No Hit
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC20.1607717041800643No Hit
GACGTTGCCTAGCGTTAGAAGCGAGCTGGAATGCGTGACCAACATCACCC20.1607717041800643No Hit
GCCCAAGGGTGGTGAGAACCGGAGAACCCCAGGACGCCCTCACTGCAGGC20.1607717041800643No Hit
GACCTGGAAGCCCAAGCGCCCACGGTCTGGGCGGCCCACATCAGGGCCAC20.1607717041800643No Hit
GGCAAGAGGAGGAGGCAGCCCAGCAGGGGCCTGTGGTGGTGAGCCCTGCC20.1607717041800643No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC20.1607717041800643No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA20.1607717041800643No Hit
AGCACACTCTGTGCGGAGACTGGGCGGCCGGCCGACCCTTCCTGTCGCTG20.1607717041800643No Hit
ACAGGGGATTCACCACTTTCTTAGCCTCCTGCTTCTTCACGACAGCTGGG20.1607717041800643No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20.1607717041800643No Hit
GAGTAATCTTTCCCAAAACTCTCTCAAAATTGCGAGCCTTGGTCATGGCC20.1607717041800643No Hit
AGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAA20.1607717041800643No Hit
GTGCCTGGCAGTGTGCAGCAGGAGAGCCTTGGGTAGGGCAGGGGTTTGCA20.1607717041800643No Hit
GTGTGACGTGGACATCCGCAAAGACCTGTACGCCAACACAGTGCTGTCTG20.1607717041800643No Hit
TGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTG20.1607717041800643No Hit
CCCTGAACCTGAAATCACACCATGGATGATTTTGAGCGTCGCAGAGAACT20.1607717041800643No Hit
GTTCTATTAACATCTGGGAGAAGAGCTGTTCCCACATCAGGCACAGCAGC20.1607717041800643No Hit
GACCACGATGACTGAGTCCCCGCGCAGGAACATCTTGGAGATGTAGCGGT20.1607717041800643No Hit
CAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGA20.1607717041800643No Hit
GTACATGGGACAGGGTGATCCTCACAGAAACACTCAAAGCCTTCTGTTAC20.1607717041800643No Hit
GTCCTTTGCTGAAGCTGAGGCTCCAGAGGGGGAGTCACTGCCACGAAGGT20.1607717041800643No Hit
GTCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT20.1607717041800643No Hit
GATCAGCACTGGGATGACGATGAGCAGAATGGTCATGAGGATGCCCAGAA20.1607717041800643No Hit
GGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTC20.1607717041800643No Hit
AACCAACCCTATTCCTGGATAGAAACAAACACAACGCAGAACCACTAAGA20.1607717041800643No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC20.1607717041800643No Hit
AGGTGCATTTGCACTCTTTGCATTTGCAGGAGCCGGCGCAGGTGCAGGAG20.1607717041800643No Hit
CTCATAAACATACCTGCTTACTATCTGGCAGGCGTCCCAGCAGTCTGGTG20.1607717041800643No Hit
CAACTCGGTCCTGGCCTGCAACCGGGCGTCCTACGAGTACGGGGCCTCGT20.1607717041800643No Hit
AGGCAAATCTATGGCCATCATGGGCCCCGGGAGGACAGCGGCCAGGACAC20.1607717041800643No Hit
GGTCAGGGTTGTATGGAAAAAAAATTCCAAGTTTGTGCAGGTCACTCTTT20.1607717041800643No Hit
ATCGAGACCATCCTGGCCAACATGGTGAAACCCCGTCTCTAATAAAAATA20.1607717041800643No Hit
GGTGGAGCTGTCCCAGCTGCGCGTCGCCAAAGTGACAGGCGGTGCGGCCT20.1607717041800643No Hit
GACTTGGTGTGCTTTGAGGAATTGACAGATTACCAGTTGGTCTCCCCTGC20.1607717041800643No Hit
GTCATGCCGGTGGTGGTGTGCACATTGAGCCCCGGTATAGACAGTTCCCC20.1607717041800643No Hit
TCCCTATGGAGATTCACCATGAACACCACGTCCAACGAGAAATCGTATTT20.1607717041800643No Hit
GTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTC20.1607717041800643No Hit
GTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCG20.1607717041800643No Hit
GACATTCCAGATGTCCTTGGGGGGCGTCACTATGGAAGGACCTTGCTCGC20.1607717041800643No Hit
ATGTGGTGGTGTCCGGGACCCCGGAGAAGATTTTGGAGCACCTTTTGAAT20.1607717041800643No Hit
CCACAGGCCAGGTGCAGACGTTTAACCCAGACAGAAGTGTTCTTGTTTGT20.1607717041800643No Hit
GACTGCGGGAGGCCAGGTTGTTTTTCACCATTCAGAACATTGCCTGAAGC20.1607717041800643No Hit
CAGGAACATCTTGGAGATGTAGCGGTCTTTGTTGACTGGCTTGGACTTCT20.1607717041800643No Hit
CATCATCTTCAGCTGCCCGCTTGCCCGTAGCTGACTCAGCTTCCTCATCT20.1607717041800643No Hit
CAGCCGCAGCTGCCATCTTAGGGGCGCCTGGCGCTACGGGTTTCTCGTTG20.1607717041800643No Hit
AATCGGAACTGGAGGAACAACTGACCCCGGTGGCGGAGGAGACGCGGGCA20.1607717041800643No Hit
GTGTGGACGGGCGGCGGATCGGCAAAGGCGAGGCTCTGTGCTCGCGGGGC20.1607717041800643No Hit
CCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCC20.1607717041800643No Hit
GCACATTCCCGTGTTCATTCACTGCATTGATGCTGTCTCTTATACACATC20.1607717041800643No Hit
GAATGTGGTGTACCCCCTGTACCGGCTGGGTGGCCCACAACTTCGGGTGT20.1607717041800643No Hit
CTTCAGGCTGCTCTGCATCTTCTTCAGCCCCTTGGCCATGTGGCTGTCTC20.1607717041800643No Hit
TGCTGCTGCTGCTTTGGTCTTCTTAGTTTTGTTCTTTTTGTCTCTCTTCT20.1607717041800643No Hit
GGTATATGGAACCCTGGGAGGAATGCTGACTCGACAGCCTTTTGCCATTG20.1607717041800643No Hit
GTTATTCGCAAGCTGGTTTTCTAGACCTGTTAGCTGGAAGCATGGTGAGC20.1607717041800643No Hit
GTGAATCACAAGGAAATAAAGATGAGTAAAAGCAAAGATGATGCTCCTCA20.1607717041800643No Hit
TGCTAGAAGTCATCAAAAAGCTATTAGTGGGAGTAGAGTTTGAAGTCCTT20.1607717041800643No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)