FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTT40.3215434083601286No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT40.3215434083601286No Hit
GTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT40.3215434083601286No Hit
TTGCCAGGGTGATGTTGGTCACGCATTCCAGCTCGCTTCTAACGCTAGGC40.3215434083601286No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC40.3215434083601286No Hit
GTCTTATAGTTGAGAATTTATCCTCAAGAGTCAGCTGGCAGGATCTCAAA40.3215434083601286No Hit
GGGGAGAAAGTGCTGTGCTTCGAGCCTGACCCCACCAAGGCGCGAGTGCT40.3215434083601286No Hit
TAGATTGGGACCACCACCTGCCTCCACCAGCTGCCAAGACTGTGGTTGAG30.2411575562700965No Hit
GAATAGCTCTAGGGATCTCAGCAGGGGTGGGAGGAACCAGCTCAACCTTG30.2411575562700965No Hit
CTTCGAGTGCGAGGCGAGGTGGCTCCTGACGCTAAGAGCTTCGTGCTGAA30.2411575562700965No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT30.2411575562700965No Hit
ACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGT30.2411575562700965No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT30.2411575562700965No Hit
TGCCCGGATCCCACTTTCAGGCCGCAACCCTCTGTTTCTGTCTCTTATAC30.2411575562700965No Hit
CGATTGCCGAGCTTAGAGCGTATCTTCCGGGATCGGGCAAAGGGGCAACT30.2411575562700965No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC30.2411575562700965No Hit
GTCTTGTAAAAAGTTTTTTTATCAATGGTTTTCAAGCTTTCCATAACACA30.2411575562700965No Hit
CTCCCACATCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG30.2411575562700965No Hit
GCCGTAGACACCAGCTCCGAAATCACCACCAAGGACTTAAAGGAGAAGAA30.2411575562700965No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG30.2411575562700965No Hit
GTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGG30.2411575562700965No Hit
GAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCC30.2411575562700965No Hit
CTCCCAATCAGTTCAGTGACCATCGGGATGATCTGCTTGACATTTTCATC30.2411575562700965No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG30.2411575562700965No Hit
TCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCATGTGCCACCA30.2411575562700965No Hit
CCTACTCCTCAATTAAGTAAATGAGAATGATTCAGCCAACAAAGTTCATG20.1607717041800643No Hit
CCATCTGGCAGGGATATGGCAACATGTGAAATCTTCACAGGACTCTTGTT20.1607717041800643No Hit
GGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCA20.1607717041800643No Hit
GGTTTAATCTTGATATTAAATTTCTTATGTGATTCTTCTTCTTCTTCCGA20.1607717041800643No Hit
GGGCAGCAGGAACAGCAGCTCTTCTTGCAGGAGGTGCATTTGCACTCTTT20.1607717041800643No Hit
GGCCTGGAAGGCCTCGGCCTGCAGGCGTATCTGTTGGGCCTGCTCCTCCA20.1607717041800643No Hit
CTTCTGAGTCATGCCAAAAGCGCCAGAGAATCCAGAACCACATGAGTCCG20.1607717041800643No Hit
ACTCTCTGCTTGTGGGTTTTGGATGCCTTCTTTAGGATCCTTTCCATTTG20.1607717041800643No Hit
ACCTTCTTCTTCCTCCTCCTCTTCCTCCCCACCTTCTTCCTCTTCTTCGT20.1607717041800643No Hit
CATCAATGCAGTGAATGAACACGGGAATGTGCCTGTCTCTTATACACATC20.1607717041800643No Hit
CAACTCTTAAGGCTGGAATGGGCTTATTTTTCACTGTTTTAGGAAATATG20.1607717041800643No Hit
GCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAAACCCCTGAAGC20.1607717041800643No Hit
GAACAAGCAGCTCCGGGAGGCTCCTGCGCGGCGGCCACCGGGATGAGCAG20.1607717041800643No Hit
CTTAGTGATAAACAATTCAGTGTCTTTCAACGGAAACATTCCTTTTAAAA20.1607717041800643No Hit
GAAGAAGGAGGAACCCAAGTAGCTTTGTGGCTTCGTGTCCAACCCTCTTG20.1607717041800643No Hit
AAGAAGGAGGAGCTGCTGAAACAGCTGGACGACCTGAAGGTGGAGCTGTC20.1607717041800643No Hit
GGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGT20.1607717041800643No Hit
GCTCCTGCTCGCCTGTTGGCTCCTGTGCCTGTGCCGGCTCCTGCAAATGC20.1607717041800643No Hit
GGGCAAAGAAGCAGAGCTAACGAGGAAAGGGATTTAAAGAGTTTTTCTTG20.1607717041800643No Hit
GATCTCCAACCTCACCGCGGCTCGAAATGGACCCCAACTGCTCCTGCGCC20.1607717041800643No Hit
AAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCG20.1607717041800643No Hit
GACGTGGTGGGTCCTCTGGTGCGAAATTCCGGATTTCCTTGGGTCTTCCG20.1607717041800643No Hit
GTGTACTTCCGGTCGGCGCCTTCGTCATGGAGTGCTTTGCCCTGCCCGCT20.1607717041800643No Hit
GAGCAATAGCGTCTGTGGTGAAGCGCCTGCACTCCTCGGGAGACATGCCT20.1607717041800643No Hit
GTTCCAATGCCAGCCCCTGAACCAGCCACACCAACTGTGGCTGCCCCAGC20.1607717041800643No Hit
ATGCAGCCCTATGGCCGCTCAGACCAAGGCGCAGAGCAACTATCAGGGCA20.1607717041800643No Hit
CATCCAAAGCATCTTCCATTGCTGATAAGTCAGAAAGTTCCTCCTGTAGC20.1607717041800643No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA20.1607717041800643No Hit
GACAAAAATTTGTTTCTAGGCAGGAAACAGGAAACTGGGGAAGCCAAGTG20.1607717041800643No Hit
CAGGTGCAGGAGTCACCGGCGGCGCAGGAGCAGTTGGGATCCATGGCGAG20.1607717041800643No Hit
CTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCT20.1607717041800643No Hit
GCCCTCCCGTGTGCCCAGCCCACAGTCTCCACTGGTGGGCACACACACAC20.1607717041800643No Hit
GTGTACGCGCTTCAGGGCCCCCATGTACTCTGCGTTGATACCTGTCTCTT20.1607717041800643No Hit
CTATATCACCTTGAAGAAGTATGACGGTCGAACCAAACCCATTCCAAAGA20.1607717041800643No Hit
GGTATCAACGCAGAGTACATGGGGTGCGGCTGCTGCGGCTCCGGGCGGCT20.1607717041800643No Hit
ATACAGAGTTGTTCGGCTGGAAGGTAGTCAAACGGGAAAACAAATCGCCA20.1607717041800643No Hit
GCACTTGTCCGACGCCCCTTTGCAGATGCAGCCCTGGGCACACTTGGCAC20.1607717041800643No Hit
GCTCTAACCGCTCGCCTCAGGCCCCTCACGCGGCCAGTTCCCCTCGCCTC20.1607717041800643No Hit
CGTCACAAGGGTGCGAGGAAAGTCAGTGAGCAAATCGCGGACCACCGGGG20.1607717041800643No Hit
CGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGAC20.1607717041800643No Hit
CCATGTCATCTCCCTGGGCCTCCAGCTCAGGATCTTTCACTTCAAATTCT20.1607717041800643No Hit
CTCCTGACCTTGGTTTCCCAAAACGAGACAGAGGCCAATGGCTGCCCCAG20.1607717041800643No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20.1607717041800643Clontech Universal Primer Mix Long (96% over 26bp)
CCTCAGACCAGTGACAAGGGCAGACTGACCAGGGGCTGTGGGGGGATCCC20.1607717041800643No Hit
CCCCGCGAGCACAGAGCCTCGCCTTTGCCGATCCGCCGCCCGTCCACACC20.1607717041800643No Hit
ACCTTGTAGTCCGGCTTCTTGATCCTCACGTTTCTGTGATCTCTTCTCTT20.1607717041800643No Hit
TCGCCGCTCTGTCTCATTCCCTCGCGCTCTCTCGGGCAACATGGCGGGTG20.1607717041800643No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC20.1607717041800643No Hit
GTCTGAAACAGCAGCTTCTGAGGAAACCTTGTTCAACGCGTCCCAGCGCA20.1607717041800643No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA20.1607717041800643No Hit
CTTAGCTGGGACCTGGAATCGTATCCTTCTGTGTTTTTTCAGACTCCTTG20.1607717041800643No Hit
CTGTCTGGTTGTGAGCAGGGTCTGCACGAAATAAAGCTCCTGGGCAAGGG20.1607717041800643No Hit
CACTAAGGGCGACTACCAGAAAGCGCTGCTGTACCTGTGTGGTGGAGATG20.1607717041800643No Hit
CCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCA20.1607717041800643No Hit
CCACATGGCCAAGGGGCTGAAGAAGATGCAGAGCAGCCTGAAGCTGTCTC20.1607717041800643No Hit
CATTAAAATGGATCAGATATTCTGGGATCTTTCTGCCTTTTTCGTCTTTC20.1607717041800643No Hit
GTACCGGCCGTGCGTACTTAGACATGCATGGCTTAATCTTTGAGACAAGC20.1607717041800643No Hit
GGACTGAGGTACCCAAGAGTGGCAAGGGCAAGAAGAAGTCCAAGCCAGTC20.1607717041800643No Hit
TGCTTCTCCTTGCCTCGAAATGGACCCCAACTGCTCCTGCTCGCCTGTTG20.1607717041800643No Hit
TCTCAGCAAGGGAGCTTACCCGAGAGGCAAAGGTATTTGCCTGAGTAAAG20.1607717041800643No Hit
CTGTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGC20.1607717041800643No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC20.1607717041800643No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA20.1607717041800643No Hit
GGTCGGGATGATCAGCCGCGCGAGCTTCTCCGAGGACATGGGCGAGAAGC20.1607717041800643No Hit
CAAGAGAGTCTATCCGAAGACATCCGACATGCAGTAACCATTTTGATTCA20.1607717041800643No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG20.1607717041800643No Hit
TCCCCATCGTGGGCATTGTTGCTGGCCTGGCTGTCCTAGCAGTTGTGGTC20.1607717041800643No Hit
CCACCTGGTCGGTCACCTGTCCCAAGGATTCTTCCTCCTGCTTCTGCCGC20.1607717041800643No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCC20.1607717041800643No Hit
GGCTATCGTAGTTTTCTGGGGTAGAAAATGTAGCCCATTTCTTGCCACCT20.1607717041800643No Hit
GCCAAGTCCATCTTGTTGAGGACCAGCAAGTGAGGCTTAAGCCCAAGGGT20.1607717041800643No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC20.1607717041800643No Hit
CCTTTGAGTTTTAAGCTGTGGCTCGTAGTGTTCTGGCGAGCAGTTTTGTT20.1607717041800643No Hit
TCGATAGGCACTGCAACATGGTGCTGGAGAACGTGAAGGAGATGTGGACT20.1607717041800643No Hit
CTTCCCCACTCTGCCGCAGACGCTTCTTGGCTCTGATCTCATTCATAGCC20.1607717041800643No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT20.1607717041800643No Hit
CTCCTGTAGTGCTCACAGGTCCCAGCACCGATGGCATTCCCTTTGCCCTG20.1607717041800643No Hit
CAGGGGAGAAAGTGCTGTGCTTCGAGCCTGACCCCACCAAGGCGCGAGTG20.1607717041800643No Hit
CCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTC20.1607717041800643No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACAT20.1607717041800643No Hit
CCTCAGGCCTTGACCGCGTACTTCCGCAGCGGGTACAGCCGCTCCTTCCG20.1607717041800643No Hit
CTGCAAGCTCCGCCTCCCGAGTTCACGCCATTCTCCTGCCTCAGCCTCCC20.1607717041800643No Hit
GGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTCTTCTTG20.1607717041800643No Hit
AGCCGATACCGTGAAAGATCAAAGAAAAACGGTGTGCTTCAAATTGCGCT20.1607717041800643No Hit
GTACATGGGCTGTCCGAACCCAGACACAAGTCTTCACTCCTTCCTGCGAG20.1607717041800643No Hit
GATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCG20.1607717041800643No Hit
AGGTGTGGAGGTCGAAATACGATTTCTCGTTGGACGTGGTGTTCATGGTG20.1607717041800643No Hit
GCACGTAGGCGACCTTGTAGTCCGGCTTCTTGATCCTCACGTTTCTGTGA20.1607717041800643No Hit
GCACCAGGACGCCCTCGAGGTTGCTCTGGGATCCCCCCACAGCCCCTGGT20.1607717041800643No Hit
AAGCTAGACGTAGCAGGAGTAGAAGCTATTCTCGCAGAATTAAAATAGAG20.1607717041800643No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT20.1607717041800643No Hit
CAACGCAGAGTACATGGGGCTCTCCTCCCGCCGCCCAAGATGCCGAAAGG20.1607717041800643No Hit
GTGTCTGGGGAGATACTGGCTCACCCAGGAAAACAGGGAACATCACCTGG20.1607717041800643No Hit
GTCCAGCACGGTGTTCGAACTGAAGCGCATCGTCGAGGGCATCCTCAAGC20.1607717041800643No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTC20.1607717041800643No Hit
TCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGAT20.1607717041800643No Hit
GGATGAGACAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTTAG20.1607717041800643No Hit
GAATGAGGGCCGAGTAGATGCAGGCGAGCTCGGAGACAGAGGCCATGGCG20.1607717041800643No Hit
TTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGA20.1607717041800643No Hit
CGTCCACCGTGATCCACATCCGCAGCGAGACCTCCGTGCCCGACCATGTC20.1607717041800643No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC20.1607717041800643No Hit
GGCCAGGATTGCAGAAGGCTCGCAGAGGTGCTGGGGAATCTGATTTTGTC20.1607717041800643No Hit
GTACAGAGCAATCCACTGGGAAACAAGATGCAAGACTTTACGTTTCCTAC20.1607717041800643No Hit
GAGCAGGAGCAGCAGCCGGCGGTCCGGGTGTAAGCGGCGTGTGCGTGTGC20.1607717041800643No Hit
GGTAAATGGCTGTAGATTACTGACACGCGGTCCTTTGTACGTAACAGAAG20.1607717041800643No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC20.1607717041800643No Hit
CCTGCTGCCCTGTGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAA20.1607717041800643No Hit
CCTTTTTAACCTTGGGCTTGGCTTCCCCGGAGGCTGCCTTCTTGTTGAGT20.1607717041800643No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG20.1607717041800643No Hit
GCACCCAAGAGAAAGGCACAAGCTGTTTCAGAAGAAGAGGAGGAGGAGGA20.1607717041800643No Hit
GGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGC20.1607717041800643No Hit
GGTGGGCCTAGCAGTCGCTCCATTTATCGCTTGAGATCTCCAGCCTTACC20.1607717041800643No Hit
GTTGTCAGTAGTTCTTTGATGTGAAAGGGGCAGCACAGTCATTTAAACTT20.1607717041800643No Hit
GTCCACGACGAGACCAGAAGTGGCATGATAAACAGTATAAGAAAGCTCAT20.1607717041800643No Hit
TTTAGAAAGTTTCTTCCACTCTTTGACCGAGTATTGGTTGAAAGGAGTGC20.1607717041800643No Hit
GTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACG20.1607717041800643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1607717041800643No Hit
ATGTTGACGTTGGCCAGGGCCTTTGCAAACAAGCCAGGCCAAAAAGGCTC20.1607717041800643No Hit
ATCCTTTACACAGTTGGTAAAAATGACATTTTTTAGGCCTTCTCCTTCTA20.1607717041800643No Hit
TGCTGATCCACATCTGCTGGAAGGTGGACAGCGAGGCCAGGATGGAGCCG20.1607717041800643No Hit
CGCTTACTTTGTAGCCTTCATCAGGGTTTGCTGAAGATGGCGGTATATAG20.1607717041800643No Hit
GTGATGGTGAGGAAGAGGATGGAGATGAAGATGAGGAAGCTGAGTCAGCT20.1607717041800643No Hit
AACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTG20.1607717041800643No Hit
AATGCGTAGCGGGCCCAGAGCTTGGGCTGCACATCGGAGCATTCGCGGAT20.1607717041800643No Hit
CCTTTAGCTGGCTGTCCGACAGATCCCCGAGCATCTCGGCCAGCAGCCAG20.1607717041800643No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT20.1607717041800643No Hit
CATAAATGCTAGTCCAAAGCACATGCACCAACACCAGGCTCTTCTTTGGC20.1607717041800643No Hit
CCAAGATGGTGAAGCAGATCGAGAGCAAGACTGCTTTTCAGGAAGCCTTG20.1607717041800643No Hit
GGCTCCTGCAAATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTG20.1607717041800643No Hit
GTACTTGAGCTCCACTCCGCAGCGTCTGAAGTTGCTGGACGCGTACCTGC20.1607717041800643No Hit
GGAAAGCACGCTTGATCCTGTCACGAACACATTTAGCACACATGGAACCA20.1607717041800643No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)