FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT34.545454545454546Clontech Universal Primer Mix Long (100% over 23bp)
AGATTCAGCAGACGGAAAACGGTTACGGACTCGACTGGCTTTTGCGGCAG34.545454545454546No Hit
GGCTTCCTCCTCCTCACCCTCCTCGCCCTGCTGGCGCTCACCTCCGCGGT34.545454545454546No Hit
ATGTTCAACTCTGTGACTTGAATGCAATCATCACAAAGTAGTTTCTGAGA23.0303030303030303No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT23.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
GTACCAGCCAACTATTCTTTCCCTGGCATTGACTTTCTTAAACATTCCAT23.0303030303030303No Hit
ATATACATCTTCTCTTTTTCTTTTTTATTCAGGTGAAATTTGTATAACAT23.0303030303030303No Hit
GTTTCCTCAGTCCTAGCCAGGACTTCACGCTTCTTCAGTAAGAACTTCTG23.0303030303030303No Hit
CTTCCGAGCACGAAGGTGGCGAACATGACCGCGTTGAGCGCGATGCTCCA23.0303030303030303No Hit
GTTTCGTGAAGACCTCTGTACCAAGACAGAGAACCTGCTCGGGAGCTATT11.5151515151515151No Hit
GCTATAATAAAAACACCGTAGTTGTTTACAGGCTTATAAACAACAGAAAT11.5151515151515151No Hit
GCGTGAACGGGCAGCCCGGGCGCCAGAACACGACGACGCCGCCGCCCTCG11.5151515151515151No Hit
GACATTTGGAGCGCTTTCAGGCCTATGTTGAAAAAGGAAATATCTTCCCA11.5151515151515151No Hit
ATCCTTGGCATAATATGGCGCTGGTATCTCTGTCAGTATCTCTGTTTTCT11.5151515151515151No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
AGATCTCGGTGGTCGCCGTATCATTCCCATGTCTGTCTCTTATACACATC11.5151515151515151Illumina Single End PCR Primer 1 (100% over 25bp)
GCGAAACAGCGTGCGCATCATTCGGCCGTGATGTGGGCGGCTTCTATGAT11.5151515151515151No Hit
CGACAACCAAGCCATCACCCGCCAGCGCGAACACGACCAGTCGACCGAGC11.5151515151515151No Hit
CCGGCAGCTATGGCGTTGCGCTGGGCGAGGTCTGGGATACGTTGCGCGGT11.5151515151515151No Hit
TTCCTTGCTGGTCAGGACCCAGATTCATGGGATGCTTATAGTAAAGGAAA11.5151515151515151No Hit
GAAATAAGGTTTTGGGATAATAAAGATAAGAGCAAATTATGATGGAAAAA11.5151515151515151No Hit
GGAATGGACTCCTTTGGAATGGTCTAGTATGGATGCAATCGACTGGCAGG11.5151515151515151No Hit
GAATGGAATCAAATCGAATGAAATGGAATGGAATAGAAAGGAATGGAATG11.5151515151515151No Hit
CCTCCGTGGAGCTCGTCCAGGCCCACCTGGACCGCATCGCGGCCCACGAC11.5151515151515151No Hit
CTGGAGGATATTCTCATTACCAAGATTACTGCAGTTACCTTTCCTGGAAA11.5151515151515151No Hit
GTATATGTTCTTGAACAAAAATGCTATTAAAATGTATGTAACTTGATTTT11.5151515151515151No Hit
ATCCTGAGGAGTCAAAGGGTTGGTTTTGTGAGCAGATGGGTGGCAGACAA11.5151515151515151No Hit
TCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTAT11.5151515151515151No Hit
ATGTTTCTTCTATTCACAATTTGTTGAGACTTTTCATAATGAAGGGATGT11.5151515151515151No Hit
GGCGTGAATGGCATCGGTGAGCCGCCGAAGCCCGGGGAGGGCATCGGGCC11.5151515151515151No Hit
CCCTCCTCCAGTGGCTTCTCCAGCCCGCACAGCGGGAGCACCATCAGTAT11.5151515151515151No Hit
GATGAATACAATGTGGAAAGCATTGATGGTCAGCCAGGTGCCTTCACCTG11.5151515151515151No Hit
CTCCCAAAGTGCTTGGATTACAGATGTGAGCCACTGTACCCAGCGAAGTT11.5151515151515151No Hit
GGGAGGGTCCTCCTGTGCCTCCTTCTAGCTTCTGGTGGCTGCCGACAATC11.5151515151515151No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGAGAGTGTTTTGTTTAGCTCATTAATCCTCTAATCATTCTGGGAAG11.5151515151515151No Hit
TTTGTGAGCCTGGCCCCTGGGTTTGGCCACTCCCTGGGGCAGTCAGAAGG11.5151515151515151No Hit
GTATTGAACAAAGGTGTGAAAAGCCCACGCAGATTCAAGGTGAGGGGACA11.5151515151515151No Hit
ATCATATATGGGGTCACACCATCCTCACGGTAGTCCAATAGAGCAACCAT11.5151515151515151No Hit
CATATGTCCTTAATGCTCACTACATCTAATCAAAAATTCCGATAGTACTA11.5151515151515151No Hit
GCAGGAAACTATGGAAGCTGCCAAGGGGTCCACTGAAAAGTTAATTGAAG11.5151515151515151No Hit
GTTTTTAAACATTTTACAGCCATTATCCAACAGACAGTAAAGCAGTGGAC11.5151515151515151No Hit
AGATATAATAGCGGGGCTGGACGTGGTGGCTCAGACCCATAATCCCAACA11.5151515151515151No Hit
GGTCAGGAGTTTGAGACCAGCCTGACCAACATGGCGAAACCCTGTCTCTA11.5151515151515151No Hit
GTGAACCCCAGGACGTAATAGACGACTGTGCGGGCGGCCAGCGCCGCATG11.5151515151515151No Hit
GTTATCTGGCCACTCAAAGTAGAAGCTCATCTGTGAGAAGGAGAAATAGG11.5151515151515151No Hit
ACCGATGCGTTCGAGCAGTTCGGCTTTCAGTACCTTGGCGTCCTCGTCGG11.5151515151515151No Hit
CTTCGGCACCGTCGTGCGCGACGCCGCTGCGAGTGTCGGCAAGCGTGTCG11.5151515151515151No Hit
GTACTGGCTTGACAGCTGTGTGGAAATAAAGACAACTCAAACGGGAACAA11.5151515151515151No Hit
ACAGGCAACTGCTCGATCAGCGCCAGGCGCTCGGGGCTCTGTGCCTCGCG11.5151515151515151No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACAGCTGTCTCTTA11.5151515151515151Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TTGCGTGACTGTGTAAGTGATGGAAAAAAGAAGTTCCATGTGACATCTGC11.5151515151515151No Hit
TGTGTCCTATCATGGAGAAGGGGAGTCAGAGGTAAGCCCATTCTCACCCT11.5151515151515151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)