FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGCGGAAACCCACGCCGCAATCCTTGCTGCTGGGGGTGCAGGGACCC34.545454545454546No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT23.0303030303030303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTGGAACACAGTCAAAACTGAAAATGCAAAATGTGTTTGAACTAGATA23.0303030303030303No Hit
ACCCCATCTCTACTAAAAATACAAAAATTAGCCAGAAGTGTTCCCAGGCG23.0303030303030303No Hit
GAAGTCAGCCGCGTGATCGAAGCGAAAATCGGTTTATCGAGATTTTCCGG23.0303030303030303No Hit
CCTTTTCAAAGAGCAGTTTCCAATCACTCTTTCTGTGGAATCTGCAAGTG23.0303030303030303No Hit
GTACATGGGGAACTGGGCCTGAAAGGGTACCGGTGACCGCTACTGCTGCC23.0303030303030303No Hit
CTGCTCGCATGCACGAAGCTGCCGCGCCCGGCATCGACGAGCGACCGCAT23.0303030303030303No Hit
TCCTTATACTTAAGGTATCTTCATTGGACTGGAGAGGCACTATGGAGGAG11.5151515151515151No Hit
TATTACATCTTTTTGCTTTTTATTTTGTTTTGTTTTTTGAGACAGAGTGT11.5151515151515151No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAT11.5151515151515151No Hit
TTGTATTCCAGTCCAATCTATTCGATTACATTCCTTTTGATTCCCTGCCA11.5151515151515151No Hit
CTTATTTGTGATGTGTTTGCTCAACTAACAGGATTGAATGCAGACATCAC11.5151515151515151No Hit
GTATTAGGAAGTGGGACCTTCAGGAAGCGATTAGGTCATGAGGGTGGAGC11.5151515151515151No Hit
CATCCGTCCGGCTGGAATGCTATTCTTAATTGACCTGCATTTATTTATTT11.5151515151515151No Hit
AAACTAGCAAGGACTCAACGAAATAACAACTACAGGCCTATATTCCAGGT11.5151515151515151No Hit
CGCCGGCGAAGAACCGGATCCGGTGATGCCGGAGGACGCCGAGGCCGTGA11.5151515151515151No Hit
ATTAACGACAAAAGGGCAGACTTCAAACAAAGGGGCTGTGTGGTGAAGAG11.5151515151515151No Hit
GGCTCTCTCTTTCCTTGACCAGTCAACAGCCCCAAAGATACGGCTAAAGA11.5151515151515151No Hit
GCTCCTTGTCCTTCAACATGGCGATGGCTTGTTCTCTTGAAATATCCGCC11.5151515151515151No Hit
ACATGGGAATGATACGGCGACCACCGAGATCTCTGTCTCTTATACACATC11.5151515151515151Illumina Single End PCR Primer 1 (96% over 25bp)
GTGTTCAGGTTAGGGTACTGGCGTGCAGCTCCACAAACGCGACGTGGTCG11.5151515151515151No Hit
TCCGATGTACTTCTGCATTAAATTCCTTGCTTTCAGAATCATAACCAGGG11.5151515151515151No Hit
ATCGTGGTGCAGTACCGCGGCGATGCGCCCGCCTACACCGAACTGCTGGC11.5151515151515151No Hit
GGACATAAGAGATGCCTGGCCTTTGCAAACAGTGTGCCTGAAGATACCAG11.5151515151515151No Hit
CTTCGGGGACGTGCGACGGATCGGCTCGGCGGCGCTGGACCTGTGCATGG11.5151515151515151No Hit
CCACAGAGCGTGCCATGCTCCGTGTTTGTAGACCAGTGTTCCTCCTGAGC11.5151515151515151No Hit
AAGGCAATCCATGGGCGCAGGCTCCCGGAGGCATCTGGAAAAACCACGAT11.5151515151515151No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.5151515151515151Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAATTTCTTGGAGTTTTGGTTTGTGTTTTGGCTTTTAGTGAGGAATTGG11.5151515151515151No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC11.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
ACATACTCTAAGGCTAATTTATCGTCTTTTATAAGCACCAGTTTCCTCTC11.5151515151515151No Hit
CCCTGGTCACCGACGAGCTGATCGAGTTCCATCGTCGCCCAGCCGCCGGC11.5151515151515151No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTT11.5151515151515151No Hit
TCTCAGCTCACTGCAACCTCTGCCTCCAGGTTCAGGCAATTCTCCTGCCT11.5151515151515151No Hit
GGCCATGGCTATCCCACATCAGACACTCAAAACAGGCCAGCTACCATTTT11.5151515151515151No Hit
GCTTCTCTAATTTGTGCCCTGGCTTCCAACTCAGAACCAAGCAGAGAAAG11.5151515151515151No Hit
GCCATATATGCCATATGGCATGATCCAGATATTAGCTACATGACCATCTT11.5151515151515151No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGCCCATGTACTCTGCGTTGA11.5151515151515151Clontech Universal Primer Mix Long (96% over 27bp)
ACTGGTAGTTTTTGAAATTAGCAAGGATGTGCTTGATTTGTTCTGCAGCC11.5151515151515151No Hit
CGGCAGATCAGCCGGCAGGCGTTGTTGACCATCGTGGCGGCGTTGCTCTA11.5151515151515151No Hit
GGTTAATGGTTGAGATTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGT11.5151515151515151No Hit
ATTCCATTCCGTTCGATTCCATTCCATTCGATTGCATTCCATTCGATTGC11.5151515151515151No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCAGTCTCTTA11.5151515151515151Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCGAAGGACATGACGAAGCTTGTTGCGCCGATCGCGAGTGTTGATGCGAG11.5151515151515151No Hit
CTCTTCTGGTTGCCTTCATTTTCTTGGTGAATTGGGATCCTGACAGAGGC11.5151515151515151No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTT11.5151515151515151No Hit
TTGTAGGAGTGTGTTCTTGCAGGTATCATTAGGCTGTTTCCTTTACTATA11.5151515151515151No Hit
TGTCACATGTGAGACCTGTCAGACAGGACCAGGGTTCCTTGTGCACTCAG11.5151515151515151No Hit
CCACTGGAGGGCCTCTCGGCTGCAGAGCAAGCGTTTGTAGCTTGTTCGTG11.5151515151515151No Hit
AGCTGGAACTTGATCGGCGTCGGGCTCTGTGCAAAGGCCGGTGCGAGGCC11.5151515151515151No Hit
GTGCAGGTCCACTCTCAAATAAAGAATCTTCAAATCAGCTTGGAGTTTCC11.5151515151515151No Hit
CTCATAGGCTGAGGTGGGAGGATCACTTGAACCCAGGAGTTCCAGGCCAG11.5151515151515151No Hit
GTGTGCCCCTACCTGTTCTCCCCTCCAGGCCACCTGTCTCTGTAAAGGAT11.5151515151515151No Hit
GAGCCAGCCCTCGAGCATGCGGGAGGCGGCCGTGGTCGGCTGGCCGCGCG11.5151515151515151No Hit
CTCCTTGACTGGATCAGGCACTGGGATGTCCAATGGGGCCTTCAGATTGC11.5151515151515151No Hit
GAAGTCAGCCGCTTGATCGAAGCGAAAATCGGTTTATCGAGATTTTCCGG11.5151515151515151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)