FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length140
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAAGACGGTGGAGGCAAAATGTGGGAAGGGATGTTGACATCAATAGG38.333333333333332No Hit
GCTGCTGTCGGGCTTCTCGTCGGAACTGCTCGATGCCGACCGCGAGGTGC38.333333333333332No Hit
TCCTGGGCGTCGGCGCTCAGCCGGAACCTGCGCACGAGCTCCTGGAGCTC38.333333333333332No Hit
GATCGGCGAGATCCGTCGGATTCATCCGCATCTCGTTCCACATCTTGCGC25.555555555555555No Hit
GCCTGGCGCTGTCGAACGCGCTGGTGGCACTGGCCGGCGCGCTGTTCGCG12.7777777777777777No Hit
GTACAGGGCCGCGTAGAGTCGGCGATCGTTCTGGACCACGAGGTTCTGCC12.7777777777777777No Hit
AGCTTCAAGATGGCTGACTAGCGGCATCTGGTATTTGCCACCTCCACAAA12.7777777777777777No Hit
CTGGCAAAGCGAAGCCGACTCGGTGCAGAAGGGGGCAGTCGCACGCGCTG12.7777777777777777No Hit
AGCTTACGCCGAGCGGTCTGTACGCCAAGCGCCCGCTATGCAACGAGGGT12.7777777777777777No Hit
ACTTCCGAATGTAAACCACATATGTGGCTCCAATTGGTGGCTTGCATGAT12.7777777777777777No Hit
GAAAACGGAGATGTGTATAAGAGACAGGAATATCGCCATGAGTATTCCCC12.7777777777777777No Hit
GAAATCGGAGATGAGTATAAGAGACAGGAATATCGCCATGAGTATTCCCC12.7777777777777777No Hit
GACTTCTAAAATAGGACCCAAAAAGCTATAAACATTAAAAAAATTTTTTC12.7777777777777777No Hit
GAACCACAGCAGCAACAAAATCCCAGATCCCATATAGAACTAACAATTAA12.7777777777777777No Hit
GGCTAGTCTCGGCCGAATCGTAAATTCGGACGGGTGCTCGATCATGGTTC12.7777777777777777No Hit
GCTCGCGGAACGCGCGCTCGCCGTCGAAGCGGTCGGCGCGCGCGGCGGTC12.7777777777777777No Hit
GTGCTTTCTCAGGTTGAGGGAGCCTCCTCCAGGGCTCGGGTACCAAGGCA12.7777777777777777No Hit
GCGTAACTGCGGGCCCGCCGAACTGGGGGCGGGACATGGGCAGTTTTTCC12.7777777777777777No Hit
GCTAGGCCCTGGGCAGGGCTGGCAGATGAGTTTGGGGACAGCTCAAGGTC12.7777777777777777No Hit
ACCTGGAGCAGCGCGGCGAGGAACTGCTCGCGCGCGGCGTGGTGGCGGAA12.7777777777777777No Hit
CCTGTGGTCCCAGCTACTCGGGAGGCTGAGGCAAGAAGATTGCTTGAACC12.7777777777777777No Hit
CCCCCGTTACTGGTTTGCGTATTAAGGATTCCGGCAATCGTCCAGTTCAT12.7777777777777777No Hit
ATATGGAGGAACACCGGTGGCGAAAGCGGCTCTCTGGCTTGTAACTGACG12.7777777777777777No Hit
GACCTTGAATCGTACCCTGTCAGAAGGTCAGCCTTGAGGATTGTCAACCT12.7777777777777777No Hit
GAACTCGGAGATGTGTATAAGAGACAGGAATATCGCCATGAGTATTCCCC12.7777777777777777No Hit
AGGGGCGTTCGCGGCGCCAGCTGGCACAACTTCGCGACCGGCGACAAGGG12.7777777777777777No Hit
GGACGACACCACCACGACGCGCAGCGGGGTCGACGCGAGCCCGGCGACCA12.7777777777777777No Hit
GTATACCCCCTCCCCAGTTAGACCCTCCATGGGTTCCTGGAAATAAATCA12.7777777777777777No Hit
CTGCTCTATCAAAAGAAAGCTTCAACACTGTTAGTTGAGGGTGCACATCA12.7777777777777777No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)