FastQCFastQC Report
Tue 10 May 2022
EGAF00003643277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6741
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG320.4747070167630915No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG220.32636107402462544No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC210.3115264797507788No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG150.22251891410769914No Hit
GTATATAGGCTGAGCAAGAGGTGGTAAGGTTGATCGGGGTTTATCGATTA140.20768431983385255No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT120.1780151312861593No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG120.1780151312861593No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA110.16318053701231272No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT100.1483459427384661No Hit
GTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGC100.1483459427384661No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA90.13351134846461948No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC80.11867675419077288No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA80.11867675419077288No Hit
CATGTGGAGAGCCTACTCTGACATGAGAGAAGCCAATTACATCGGCTCAG70.10384215991692627No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG70.10384215991692627No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT70.10384215991692627No Hit
CCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGC70.10384215991692627No Hit
ATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTCTGGGG70.10384215991692627No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)