FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003643281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length140
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT28.0No Hit
GTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACG28.0No Hit
TTGTCGTACTTGACGCCGTGCCAGCCCTCGATGACCTGCGGGCCGGAGGC28.0No Hit
GAATAGGGCGGGCAGGGCCCAAAGGACTTGTTCAACCAGCAGCCGGACCG28.0No Hit
CATTTATTTTGGAATGTGCTAGAAACAGCTTGAAACATCCCTTTAATAGC14.0No Hit
CATAAATCCTTGTTTGTCGATCCTGTTCTACCTTTTAAATGATGCCTCTC14.0No Hit
CACACACACACACTCACACACACACACACACACACACACACACACACACA14.0No Hit
TTCGATGGTGTCTCTGGGCTCCACCTCCAGAGTGATGGTCTTGCCGGTCA14.0No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT14.0No Hit
CCAATCAAGCTGATTAAACTTGCTCCTACAACTGGACCTAAAAGATCTGC14.0No Hit
GCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGG14.0No Hit
GTCGGGCTGGTTGGCCTCCTTCCGGTTGAAAACCTTAATATTGACCCGTT14.0No Hit
GTACATGGGGCGTCCGGCGGCGGCCTCTCGCGCCGGTCGGCGCCGTCGCG14.0No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG14.0Clontech Universal Primer Mix Long (96% over 26bp)
GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACG14.0No Hit
GGCATAATCTTCAAAACCTCATCCTTGAGAGAGGCCCCCAGGAAGAAATC14.0No Hit
AGATAGTTCTGGGGCCTCAGGGGGCTCATGTCCATGTCCATCGAATCTTC14.0No Hit
GCCCCGAGGGGCTCTCCCCGGAGGCTCAGCCCCCTCTGCTCCCCATGGGC14.0No Hit
GGGATGTAGTGTGCCGGGTCCGAGCCCCCCAGGACCAGCTCTCCTCCATC14.0No Hit
GTTCACCCACTAATAGGGAACGTGAGCTGGGGTTAGACCGTCGTGAGACA14.0No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA14.0No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)