FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003643282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length140
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAGCGCTGACCGCCTCCACCGCCGCCGGCCTCGATGCCGTGTCGCAG28.0No Hit
GACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAG28.0No Hit
TGACAACCTCGCCCGCTTTCGGCTGATGTGCCGGCAGTTCCGCGCGCTTG28.0No Hit
AGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCA28.0No Hit
GGATAAAGAAGGCATCCCTCCCGACCAGCAGAGGCTCATCTTTGCAGGCA14.0No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT14.0No Hit
GCCGTGGACGGGGCCGGGGCCGAGGCCGCGGAGCTCGCGGAGGCAAGGCC14.0No Hit
CGATAGAATTCCAGAATCTCCGGCAGTTTTTGTACCTCAAGAAGTAAGTG14.0No Hit
CAAGTTTGCCATTCAATATGGAACTGGGCGGGTAGATGGAATCCTGAGCG14.0No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14.0No Hit
TGACCCAGAGGTGCGGGTTCTACATAGCATCGGTCACAGTGCTGCCCCAC14.0No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG14.0No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC14.0Clontech SMART CDS Primer II A (96% over 26bp)
ATCTCCGTCCGCCTTCTCTCCTACCTAAGTGCGTGCCGCCACCCGATGGA14.0No Hit
CCTTCGTGCGCTTCACCCGCGTGGAAGGCGAGCAGGGCGAGCGCCACGTG14.0No Hit
GTGGTTTGGACACCTCGCTCCCAAAAAACATGGGCCAGTTCTACTGAAAA14.0No Hit
GTGGAGCCGATGGAGAAGGTGGCAGTGGTAACCAGGTGCTCACTCAGGGC14.0No Hit
GCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTC14.0No Hit
GTCCCATTCCTGGGACACAGGGTCAGCTGGCCAAAAGATCTCAAAAAAGA14.0No Hit
GTGTGTGTGTGTGTGTGTTAGAGTTTGAGATTGTGAGTCTGAGTGAGTGA14.0No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA14.0No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)