FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG43.0303030303030303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG32.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
GCTCGATGCCACGCGCCGTCTTTTCGTACGCGGCGAACAGCTCTTCGGGC32.272727272727273No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA21.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
GAAGAGCGTCGTGTAGGGAAAGAGTAGAGGGGTATCCCATGTACTCTGCG21.5151515151515151Illumina Single End PCR Primer 1 (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.5151515151515151Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAAT21.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
ACACGACGCTCTTCCGATCTCTGGTACCTGCTGTCCCAAGTTCTGCAGGT21.5151515151515151No Hit
GAGTACATGGGAAGCAGTGGTATCAAACGCAGAGTACTTTTTTTTTTTTT21.5151515151515151No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT21.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTC21.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACACTGCTTCCCATGTACTCTGCGTTGAT21.5151515151515151No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGCG21.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTG21.5151515151515151Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC21.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21.5151515151515151Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACATGGGAAGCAGTGGTATCAACTCAGAGTACATGGGAACAGTGGTAT21.5151515151515151Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GATTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG21.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
ACAGCGGTCAAAGCCGTAGAACTCACTAAGACCTTCGTTTTTACTGGTTA21.5151515151515151No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
AAAAGAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
TAGCAGCTGACTGGGAAGAGTTCTGTGGAAATGGCTGAGGCTGAGGAGAA10.7575757575757576No Hit
TCAACGCAGAGTACATGGGAAGACCGATACATCTTTCCCAGCAACTCCAG10.7575757575757576No Hit
AAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTT10.7575757575757576No Hit
GATCAAGTCCCCTTCGGCGGCGCTGAGATCGTCGGCCGGAATCGGTGCCG10.7575757575757576No Hit
AATCAACCAAGAAAAATAGAATAAGCAGAAATATACAATATAAACAAACA10.7575757575757576No Hit
GTCATAGGCATTGCCTGCGACGTGGGACCGCTTGTCGACCACGAGCACGC10.7575757575757576No Hit
AACAACGGGTCGACGAGTTCGGCCATGACGTCGGCACAGGGCAACAGCCA10.7575757575757576No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10.7575757575757576No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCTGT10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTT10.7575757575757576Illumina Single End PCR Primer 1 (100% over 38bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGT10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGGCGGGGCGG10.7575757575757576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTTACACTCTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGA10.7575757575757576Illumina Single End PCR Primer 1 (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACTGCTTCCC10.7575757575757576No Hit
GCACGATGCCTCCCCATTCTACTGGCATGGACACTGACCCTGCTTAGCCC10.7575757575757576No Hit
GTGTAGGGAAAGGTGTAGTCTCGGTGGTCGCCGTTCACCCATGTAGTCTG10.7575757575757576No Hit
GATCGGAAGAGCGTCGTGTAGGAGAGTATAGCGCCCATGTACTCTGCGTT10.7575757575757576Illumina Single End PCR Primer 1 (95% over 22bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACGACTGCTTCC10.7575757575757576Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGATGATCGGCGCCACCGAGATCACATCTTTCCCTACACGACG10.7575757575757576No Hit
GGTCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
TCTTTATAGTCAAAGGGTCCTGTAGAATGTGAGATAATGTACTGGAGAAG10.7575757575757576No Hit
ATGTAAGTGTTATTACATTATTAAATCATTTTGTTCCAGAGCTGAATAAA10.7575757575757576No Hit
ACGCAGAGTACATGGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.7575757575757576No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGATATCCCCACAGAGATCTACACTCTTTCCCAGCAACTCC10.7575757575757576Illumina Single End PCR Primer 1 (95% over 24bp)
ACATGGGTGATAGCGACACTCTACACTCTTCACGACGCTCTTCGATCTCT10.7575757575757576No Hit
GGTATCAACGCAGAGTACATGGGGTACATGGGAGATACGGCGCCGGATTA10.7575757575757576No Hit
CCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACA10.7575757575757576No Hit
TCACTGTGGGACTTTGAGTGACCCACTCTCCCCCTCTGGGTTTCAGTTCA10.7575757575757576No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGCCGATCTCAT10.7575757575757576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAACTGGCGTTGTTA10.7575757575757576No Hit
ACTCTGATGAAGAGAAGACGCTAGAAATTGATGTACAGGGTGAAGGAACT10.7575757575757576No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.7575757575757576Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATCGCCACC10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGCACCACGA10.7575757575757576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGAAACCCCAGCTTC10.7575757575757576No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.7575757575757576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATACCACTGCTTCCCA10.7575757575757576No Hit
CAATATACCATTGTAGTTTGGTCAGAAAAGAGAGTGCATACATGGTTCAG10.7575757575757576No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.7575757575757576Clontech SMART CDS Primer II A (96% over 26bp)
GGTTCGCGCAAGGCATCGCGCGTGAACAAGCCCGAAGCCGGCCACCTCGC10.7575757575757576No Hit
ATGTTCATCATCTGCGTGAACCAGCGAAACAGCGGCGGGATGAACTCCCC10.7575757575757576No Hit
ACCGAGATCTACACTCTTTCCCTACACGGAGCTGCCGATACCAGTAAACA10.7575757575757576Illumina Single End PCR Primer 1 (96% over 28bp)
ATTTACAACACAGATCAGAAAGCTTAAAAATATTACAAGTACTAAGTCGG10.7575757575757576No Hit
CAATAAACTAATACAAAAATCCAAGCACACGAAACCACGCACACTACATC10.7575757575757576No Hit
TTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC10.7575757575757576No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GGCCTACCCCAGGCGGCCGCCTACAGTGCCGCAAAAATCGTGCTGCCCGG10.7575757575757576No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACATCCCATGTACT10.7575757575757576Illumina Single End PCR Primer 1 (97% over 37bp)
ATTTACAACACAGTTCAGAAAGCTTAAAAATATTACAAGTACTAAGTCGG10.7575757575757576No Hit
ACCCACGCCCATGCCTTGCAGGCGTCCGGCCGTCGCCTCGTTCAGCGCCT10.7575757575757576No Hit
TCTACACTCTTTCCCTACACGACGCTCGTTTTTCCTGTCTCTTATACACA10.7575757575757576Illumina Single End PCR Primer 1 (96% over 29bp)
GTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACATTCCCATG10.7575757575757576Illumina Single End PCR Primer 1 (97% over 40bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.7575757575757576Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGTA10.7575757575757576Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAACAGG10.7575757575757576No Hit
GTACATGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAT10.7575757575757576No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTG10.7575757575757576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCTAAAATATAAACATAAACGACCCAAATACAAAAACCACACTACAAA10.7575757575757576No Hit
GTGTAGGACTGGGTCCTGATCGAGGGTTTCCATGGAGCCACCAAGATCGT10.7575757575757576No Hit
GAGGCAAGGAGAGCGTCGTGTAGGGAAGGTGTAATCTGGTGGGCCTACCC10.7575757575757576No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGACTACATGGGAAGCAGTGGT10.7575757575757576Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AACCACTGCTTCCCATGTACTCTGTATCAACGCAGAGTACATGGGAAGCA10.7575757575757576No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG10.7575757575757576No Hit
AAAAAGTACTCTGCCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.7575757575757576No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GCGTCGTGTAGGGAAAGAGTAGATCTCGGTGGTCGCCGTATCATTCCCAT10.7575757575757576Illumina Single End PCR Primer 1 (96% over 30bp)
CCATAACATAAAATGAGGGTACTACATACAATTCTCACATCAAATTAGGC10.7575757575757576No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCCTGTCTCTTA10.7575757575757576Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGTGGTATCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAG10.7575757575757576No Hit
GTGCAGGACGACGGCCTTCGGATCTGCCTTTGCGAGCAGCTCCTTGTTGA10.7575757575757576No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.7575757575757576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTTTAACAAAG10.7575757575757576No Hit
CCCTGGTACAGAAGTCCTCTAAGGTCCTCCTGACTTGCTATTTTAATAGG10.7575757575757576No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATCG10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
CACTTAAAAAAGCAAACAACATGGAAGAATATTAAACAAATCACAGAACA10.7575757575757576No Hit
CATGGGTGAGGGTCCCGAGATCTCTTTCCCAGCAACTCCAGTCCAAGCAT10.7575757575757576No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACACAGGCGTTTATA10.7575757575757576No Hit
CATGGGAACGGACCCAGTCTACACTCTTTCCCTACACGACGCTCTTCCGA10.7575757575757576Illumina Single End PCR Primer 1 (97% over 34bp)
CCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTTCTGGT10.7575757575757576Illumina Single End PCR Primer 1 (100% over 42bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTCTAAGTTGCTATGAAAATGGGTCCCGGTAGCTCCTTGTATCTCA10.7575757575757576No Hit
CTTCCGATCTCTGGTACAGACAGAATCTGAGGCAGAGCGTCGTGTAGGGA10.7575757575757576No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT10.7575757575757576Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGACGGCCACG10.7575757575757576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTAGGGAAAGAGTGTAGATTCGGTGCGCCGACTTCCCATGCTGTCTCT10.7575757575757576Illumina Single End PCR Primer 1 (95% over 21bp)
AGCGTCGTGTAGGGAAAGATGTAGATCCGGGCGCATCACCCATGTACTCT10.7575757575757576No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)