FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG96.8181818181818175Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43.0303030303030303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG32.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
ACCTGAACCGCGTCAAGCTGTTCACGACGATCGACAATCCCGATGCCGCG32.272727272727273No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC32.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.5151515151515151No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTG21.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACCCCATGTACTCTGCGTTGATACCACTGTTCCC21.5151515151515151Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTC21.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
CCCTACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGG21.5151515151515151Illumina Single End PCR Primer 1 (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT21.5151515151515151Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGTATCAACGCAGAGTACATGG21.5151515151515151No Hit
GCGCTGTTTCGTCTTTTTGGACGAATGTTGCACCTTGCACCGCAGATGTT21.5151515151515151No Hit
GATCTCGTGGTCGCACCCATGTACTCTGCGTTGATACCACTGCTTCCCAT21.5151515151515151Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAATACCCGTCTACACTCTCCCCAGCAACTCCAG21.5151515151515151No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA21.5151515151515151Clontech Universal Primer Mix Long (95% over 21bp)
GGTTGTACGTCGGAATGTTGTAACTACTAGGGCTTAAGTCGTGAACGACT10.7575757575757576No Hit
GTACTTTTATTTTTTTTTTTTTTTTTTGGGTTTATACCAATTCTACCAAT10.7575757575757576No Hit
CATGGGAAGTCGGCGCACCGAATCTACACTCTTTCCCTACACCTGTCTCT10.7575757575757576Illumina Single End PCR Primer 1 (95% over 21bp)
GTGGTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCT10.7575757575757576Illumina Single End PCR Primer 1 (100% over 25bp)
TCCCTACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTG10.7575757575757576Illumina Single End PCR Primer 1 (100% over 25bp)
GGGAGAGTGAGACCCTCCTGAGGGCTCACCGTCCACAGGGTGACACTCTG10.7575757575757576No Hit
ACTGGAAGGCGGTCGACCCGCGGCTGCCCTCGCAGGTGCGCTTCGGCGCC10.7575757575757576No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.7575757575757576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACG10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
CAGCAGGACGTCGTGCAGCTCGGCGACGCATTGAATGCATCGCAGACGCA10.7575757575757576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT10.7575757575757576No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAATTACTCGGCGTTTTTAAACACTTC10.7575757575757576No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCTGTCTCTTAT10.7575757575757576Illumina Single End PCR Primer 1 (97% over 38bp)
TATCAACGCAGAGTACATGGGGTAAGTACGCACGGCCGGTACAGTGAAAC10.7575757575757576No Hit
GTGTAATCTCGTGGTGCATCCCATGTACTCTGCGTTGATACCACTGCTTC10.7575757575757576Clontech SMART CDS Primer II A (96% over 26bp)
TCCACCTTCTGGCCCACGGAGAAGACGCTCGCCACCGGGATCACGCCGCC10.7575757575757576No Hit
ACACGAACTCGGTCGTGGGCTGGCAGCCCGGCCAGATCCAGGACTACCAA10.7575757575757576No Hit
CAGACTACATGGGTGAACGGCGACCACCGAGACTACACCTTTCCCTACAC10.7575757575757576No Hit
GTACATGGGGCCGATCTCATGATGCAGCAACTCCAGTCCTTGCATCATGA10.7575757575757576No Hit
GTACATGGGAAGCAGTCGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.7575757575757576Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA10.7575757575757576No Hit
GTCCTCGCCGAGCGACTGGCCAGCCAGCACAATGCACGCGTGCTCGTGGT10.7575757575757576No Hit
GCTCTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGCGTCGTGTAGG10.7575757575757576No Hit
GTATCAACGCAGAGTACATGGGTGATACGCGACCCGAGATCTACACTCTG10.7575757575757576No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCACG10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GCCAATATCTGTTGAGCACTGGCTGCCTTTGTGGCATTGAGCTGAGGAGG10.7575757575757576No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
CCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGT10.7575757575757576No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7575757575757576Clontech SMART CDS Primer II A (100% over 22bp)
GGGAAGAGGTAATCCGGCGCCGTATCTCCCATGTACCCCATGTACTCTGC10.7575757575757576No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATGGGTTTAAAAAAA10.7575757575757576No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA10.7575757575757576Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAA10.7575757575757576No Hit
GTGTTGGTCGTCGCACCGGTGTTGTTGACGTTCTGGTTGCTGGTCATCGT10.7575757575757576No Hit
GGGCTGGAAGATCGCCGGCGCACCGTTTGCGACGGTGACTTGCTCCGCGA10.7575757575757576No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAACGGCGTTTAAA10.7575757575757576No Hit
TCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGTAATGATACGGCGACCACCGAGATC10.7575757575757576Illumina Single End PCR Primer 1 (100% over 24bp)
CATATCCATGTACTTTTTAAAAAGTACATGGATATGTGTAGATGGGTAAG10.7575757575757576No Hit
TATCAACGCAGAGTACATGGGACGGCGCACCGAGATCTACACTCTTCCCA10.7575757575757576Illumina Single End PCR Primer 1 (95% over 22bp)
CCGTACCCATGTACTCTGCGTTGATACCCTGCTTCCCATGTACTCTGCGT10.7575757575757576No Hit
GAAAAACGAGCGTCGTGTAGGGAAAGAGTGTAGACTGTCTCTTATACACA10.7575757575757576Illumina Single End PCR Primer 1 (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATAACACTGCTTCCC10.7575757575757576No Hit
GTACATGGGTGTCGGCGACACCGAGATCTCACTCTTTCCCTACACGACGC10.7575757575757576Illumina Single End PCR Primer 1 (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGTACGCGACC10.7575757575757576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCACTAGGTGATAAATTGCACAAAACACAAAAACAATTTTTTTTTTTTTT10.7575757575757576No Hit
GTACTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT10.7575757575757576Clontech Universal Primer Mix Long (95% over 22bp)
CCGCAGGCCGGCGTCGACGGTGCCGACGCGTTGGTCACCGATACCTGGAC10.7575757575757576No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA10.7575757575757576No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGAGATCTCGGTGGTGGAT10.7575757575757576Illumina Single End PCR Primer 1 (96% over 32bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGT10.7575757575757576Clontech Universal Primer Mix Long (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAGTACACGGCTTTTTAAACCACAG10.7575757575757576No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.7575757575757576Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
CCCTACACGCGCTCGTTTTTCAGGACCTGTTGGTACCACTGCTTCCATGT10.7575757575757576No Hit
CTCTGAAATTGTAGTAGTAAGGGTCTGGGGAGAAGCCACATTTTTATCTT10.7575757575757576No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTT10.7575757575757576No Hit
GTACTTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA10.7575757575757576Clontech Universal Primer Mix Long (95% over 24bp)
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGT10.7575757575757576Clontech Universal Primer Mix Long (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GATCTACACTCTTTCCCCAGCTACTCCAGTCCTTGCATCATGAGATCGGA10.7575757575757576No Hit
TCTCGGTGTCGCCGCACCCATGTACTCTGCGTTGATACACTGCTTCCCAT10.7575757575757576No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG10.7575757575757576No Hit
GTGTAGGGAAAGATTAGATCTCGGGTCGCTACCCATGTACTCTGCGTTGA10.7575757575757576No Hit
GTACATGGGTGGCGGCCCCCAACAAAAAAAACGTTTTACACAAACCACCC10.7575757575757576No Hit
GGTTGTAGGTCGGTATGTTGTAACTACTTGGGCTTAAGTCGTGTACGACT10.7575757575757576No Hit
ACTCAGCACATTCCCACTGCCCGAGCAAAATCAGCCCTCACGGGTGAGCA10.7575757575757576No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCGCT10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACAAAGCGTTTATACCA10.7575757575757576No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.7575757575757576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACAGGGCGGGCCCCCAGATAAAAAATTCCCACACAACCCACAAACCAA10.7575757575757576No Hit
TGGTTGATCGGCGACCACCGAGATCTACACACTTTCCCAACACGACGCAC10.7575757575757576Illumina Single End PCR Primer 1 (96% over 30bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCA10.7575757575757576Clontech SMART CDS Primer II A (96% over 26bp)
CCTCTCTTGTTTGCGTCAGCAGGCGTATACCCACGTCCTCTTTGAGCAGT10.7575757575757576No Hit
GCCATGTGGCAAGTCTTCCAAGGGAAGCAATGCACTGCTTAACTCAGCAG10.7575757575757576No Hit
TTTCAAAACAGCTTTGTACAAATGTTATAAAACATATTTAATGATTAAGA10.7575757575757576No Hit
GGTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCTAC10.7575757575757576Illumina Single End PCR Primer 1 (100% over 27bp)
CCCGTCAGAGGAATAGCAGTGTGGCAGCAGCCCAGCTGGTCCGCAACCTT10.7575757575757576No Hit
CCTCTAGACTTCCTTGCCTCAGGGGCAAATTCTTCCACTCTGCTTCTAGC10.7575757575757576No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAAAC10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATTAC10.7575757575757576Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GCTCGGTGGCGATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
CCCTGCTGGTACCAGTAGACAGTGAGATCGGAAGAGCGTCGTGTAGGGAA10.7575757575757576Illumina Single End PCR Primer 1 (100% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATCTGCGTTTATACCAA10.7575757575757576No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)