FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG63.4482758620689653Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42.2988505747126435Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT31.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21.1494252873563218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTTTAAAAAGGTAATTAACTTTTTAAAATTGAAAAAGCGCGACTTTTC21.1494252873563218No Hit
CCGTATCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT21.1494252873563218Clontech Universal Primer Mix Long (96% over 26bp)
CGATTAACCTGCCGAAATAGCTAATTTTTTGGAAATACTGTTATGACATT21.1494252873563218No Hit
ATCTTATCCAGACGCATACTTGACACGATGACGTTTAGCTGCAGACTAGC21.1494252873563218No Hit
CTACACGACGCTCTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTACCAA21.1494252873563218Illumina Single End PCR Primer 1 (100% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT21.1494252873563218Clontech SMART CDS Primer II A (100% over 26bp)
CTCCCAAAGTGCTGGGATTACAGGCGTGAGCGACCGTGCCTAGCCTAAGC21.1494252873563218No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCCTGTCTCTTATAC21.1494252873563218Illumina Single End PCR Primer 1 (97% over 37bp)
ACTTGGTAGATGGTCCAGCCGTTTGGATGCCAGAACACCATGCCTGGAGA21.1494252873563218No Hit
CAGCAAACGTCTCTTTGAAGTCTGCCCAAGCATCGCTTGCGTAAAATGCA21.1494252873563218No Hit
GCTTCTAAAACAGCAGCATATCCCCATATTCCCATGGTTAGCTATTTCTT21.1494252873563218No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.1494252873563218Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.1494252873563218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTATCAAC21.1494252873563218Clontech Universal Primer Mix Long (96% over 26bp)
CTTAAAGGAACAAGGTTCCTAAAAGGAATGACTAGTTTGGGGCAGGGGCA10.5747126436781609No Hit
GTCTTGCACAAGCCAAAGAAAACCACAACGACTACGTTATAATCGATACA10.5747126436781609No Hit
GTCTTCACTCTTTCCCTACACGACGCTTGCCTCAGATTCTGTCGGTACCA10.5747126436781609Illumina Single End Sequencing Primer (95% over 23bp)
CTTGCCTCCCGTGCCGCCGTGGACCTTTCCGCGAGCGTCGAGAGCGCCTG10.5747126436781609No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGACTCGGTGTCCTCCCATG10.5747126436781609Illumina Single End PCR Primer 1 (96% over 33bp)
GTGTAGATCTCGATGGTCGCCGTATCATTCCTGTCTCTTATACACATCTC10.5747126436781609Illumina Single End PCR Primer 1 (96% over 29bp)
TCCTTTTTCTTTTATTACCTTTCCCTTCCATTTTCCTTTTCCGTTTCCCT10.5747126436781609No Hit
CTTTTCACCTTTGAAACTCAAGGCCAACAGTGGAACGAAGCCAATCAAGC10.5747126436781609No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCGTTCACCCATGTACTCT10.5747126436781609Illumina Single End PCR Primer 1 (96% over 33bp)
ATAAAACACACCCGGCCGGCACACCCACGCCCAAAACCGAAAAAAATACC10.5747126436781609No Hit
GCCGAGAAGTCAAGGGTGCGGGTGAAGATGGCGTCGCGAATGTCACGGCC10.5747126436781609No Hit
GATCGGAAGAGCGTCGTGTAGGAAGGTGAGCTGGGTCGCACCCATGTACT10.5747126436781609Illumina Single End PCR Primer 1 (95% over 24bp)
GCTTCTTCCTGTTAGTGTATTTGTCACCATCAATTTCTAGTAGGCAATTT10.5747126436781609No Hit
CACCATCACCCAGTGCTTGTTGAGGTGAAAAGCCATACTATTTCCCAGCA10.5747126436781609No Hit
AGCCAGGACCGCGTGGCCGACTCCAGCGCGATCGCCGCCGAGGCTGTCTC10.5747126436781609No Hit
GATGTAACTCGGATCCCATGTACTCTGCGTTGATACCACTGCTTTCCATG10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
CCCGCATACATGGCCGCAAACATTCCCACAGGACCACCACCAACAACGGT10.5747126436781609No Hit
GGTATCAACGCAGAGTACTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5747126436781609No Hit
TGTGTGTTGTTCGGTTGCATGGTTGAAGGAGGGTCCGATGGTGGATGATT10.5747126436781609No Hit
GCTGAAGGCCTTGCTGGTGCTGCTGGTGGTGGCCGCGTGGTGGCGCCAGT10.5747126436781609No Hit
GTATCAACGCAGAGTACATGGGGACGCCTACATCTTCCCTACACGACGCT10.5747126436781609No Hit
TCCCCCATGTACTCTGCGTTGATACCACCTGCTTCCCATGTACTCTGCGT10.5747126436781609Clontech SMART CDS Primer II A (95% over 21bp)
ATTGAACCTTGTGTCATGTGCAGCGGTGCTATTGGATTAGCTCGCATTCC10.5747126436781609No Hit
GTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA10.5747126436781609No Hit
AGACCCTGCCGAAGCCTTTTCATCCTTCTTCGCGGAATCAGCTTTAGACG10.5747126436781609No Hit
GGATCTATTGCTAATGGATTACATGACCAAAGCTACTCTTTAGTTTTAAA10.5747126436781609No Hit
TTCCTACAGGCTTTTGCTTTCACGTTCACACTGTCTATTTTTGTTTTTAT10.5747126436781609No Hit
CCACGAGTCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCC10.5747126436781609Illumina Single End PCR Primer 1 (100% over 36bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGATGCACCGAG10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAGAGTACTT10.5747126436781609No Hit
ATTTATGGTGTGATAATATTATTTTGCCTTACCCCTTTCTTATTCCTCTT10.5747126436781609No Hit
AGAATGGGTATGTGAAGACGGAGAAATCGCAAAAAGATGGTCGTTTAAAA10.5747126436781609No Hit
TTTTGGGGCAGCTTCCGATCCGTGTGATCAGTTATCGCGAAGCATTCATA10.5747126436781609No Hit
ATGGGGTCGTCCGCCAACCATGGCGTCGACGGTCCGATCGGTTTGGCCCT10.5747126436781609No Hit
GGTCTTTAGTGCAGACCACGGGATCTTATCCGGTGAGATGTTCTGCCTGC10.5747126436781609No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTTAAGCAGTGG10.5747126436781609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTGAAAGAAGTGGTGAAGCTCCCGCTCCAGGTACCAAACTCCAAGGTC10.5747126436781609No Hit
GCCGAGCATCTCGAGGTCGTCTCGAGGCTGGACGCGGGCTGTCTCTTATA10.5747126436781609No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTC10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
CATGACTACGCGACCCGAGTCTACATCTTTCCCTACACGACGCTCTTCCG10.5747126436781609Illumina Single End PCR Primer 1 (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGTGTTGA10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG10.5747126436781609Illumina Single End PCR Primer 1 (100% over 25bp)
GTACATGGGAACCACGAGATTACCCTTCCCTACACGACGCTCTTCCGATC10.5747126436781609Illumina Single End Sequencing Primer (96% over 27bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
ACCCAAAGGATCCAAATTAAAGTAGTCCAAGAAAAAGAAACAAGCAATTT10.5747126436781609No Hit
TCTGTAATCCCGGTACTTTGGGAGGCCAAGGCAGACAGATCACTTAAGCT10.5747126436781609No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.5747126436781609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGGGATTACAGGTGTGAACCACTATGCCTGGCCCACTTGCCTTTCTTA10.5747126436781609No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA10.5747126436781609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATTTTGTATCTAGGGGCCTCTCTTACAAAAGGGACCCGAACAGCCAACT10.5747126436781609No Hit
CATTTGTTCTAAGTCTGCACAAGCTTCAGAAAGGACTAGGAGTTTGGTGA10.5747126436781609No Hit
CTCTTTCCCTACACGACGCTCTGTCCCAAGTTCTGCAGGTACCAGAGATC10.5747126436781609Illumina Single End PCR Primer 1 (95% over 22bp)
TATCAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCG10.5747126436781609No Hit
GTCGTAAAATTGCGGGGACGGGAACCATCAATCCAGATGGATCAGTTGGC10.5747126436781609No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGCGTCGTGAATATCTCGTA10.5747126436781609No Hit
GTGAACCGGGGAGTGAAACTCGAGTTGTCTTGGGGCAGACGATTGGGACC10.5747126436781609No Hit
GATTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTC10.5747126436781609Illumina Single End PCR Primer 1 (97% over 35bp)
GAGTACATGGGTGATACGGCGACCACCCATAAAAACAATGATTGTAAGAA10.5747126436781609No Hit
GAGTGTAGATCTCGTGTCCGTCACCCATGTACTCTGCGTTGATACCACTG10.5747126436781609Clontech SMART CDS Primer II A (95% over 23bp)
CACCACCACACCCAGCTAATTTTTATATTTTTAGTAGAGAAGGGGTTCTG10.5747126436781609No Hit
AACTTCAACTGCATTAAATCTATTTGATTCTGGTGAGATTGACTATACTA10.5747126436781609No Hit
CCAGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGAAGATCTCGGT10.5747126436781609Illumina Single End PCR Primer 1 (97% over 43bp)
CAGTGGTTCGTCAAGATGGAGCCCCTGGCCAAGCCCGCCATCGAGGCGGT10.5747126436781609No Hit
TAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACACCCATGTACTCTGC10.5747126436781609Illumina Single End PCR Primer 1 (97% over 34bp)
GAGTACATGGGTGATACGGCGACCACCGAGATCTACACTGGGGCGGTGCT10.5747126436781609Illumina Single End PCR Primer 1 (96% over 28bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.5747126436781609Clontech Universal Primer Mix Long (100% over 23bp)
GCATGATCACAATCCTTGGAATTCCGCCGTTCTTCGACTTGGATCAGAGT10.5747126436781609No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.5747126436781609Clontech SMART CDS Primer II A (96% over 26bp)
ATCTGAGGCAAGAGCGTCGTGTAGGGAAAGAGTTAGTCTCGGGGTCGCGT10.5747126436781609Illumina Single End PCR Primer 1 (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGAACCAACG10.5747126436781609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTTGCACAAGCCAAAGAAAAACACAACGACTACGTTATCATCGATACA10.5747126436781609No Hit
GAGTACATGGGGAATGATACGGCGACCACCGAAAAAAAAAAAGTGTAGAT10.5747126436781609Illumina Single End PCR Primer 1 (95% over 22bp)
GTGAGTAATTTCCTAAACATAGCACTTTGTCCATTGCGGAAATGTTTTCC10.5747126436781609No Hit
GCGATGAAGAGCTGCTCGATCCAGTCGTCTTCCTTGGTCTGCGTCGCCTG10.5747126436781609No Hit
GAGTGTAGATCTCGGTGGTCGCCGTACATTCCCATGTACTCTGCGTTGAT10.5747126436781609Illumina Single End PCR Primer 1 (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTCTGTCTCTTA10.5747126436781609No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGG10.5747126436781609Illumina Single End PCR Primer 1 (100% over 30bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCA10.5747126436781609No Hit
CATGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GGACAATGTCCATCTCTTGGGATACAGCCTTGGAGCCCATGCTGCTGGCA10.5747126436781609No Hit
GTGTAGATCTCGGTGGTCGCGTATCATCCCATGTACCTGTCTCTTATACA10.5747126436781609No Hit
GCTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACC10.5747126436781609Illumina Single End PCR Primer 1 (96% over 29bp)
ATGTAAGCAAATAAATAGGGCGCATCAGCGATAATTGCTGAAAATGCAGG10.5747126436781609No Hit
CATTTACAGGTATGTGAAACAAGAAGTTCTGGGTCCTTTCATCATAAGGG10.5747126436781609No Hit
GGGAAGCAGTGGTATCAACGCAGAGTAAATGGGATGATACGGCGACCACC10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
TTTCAGCGATGTACCCATCGCGCTCACCGGCGAAAGTGTCCTGGCGAGGT10.5747126436781609No Hit
CTCTTCATTATTTTTATTGTTCTTTTCCTTATCGCTCTTATTTTTGGTTA10.5747126436781609No Hit
CCCTTGTTAAAGGCAGATGTGATTGAGGAAGATGCGATTATTGTTGATGC10.5747126436781609No Hit
GTCCTGTAGTACAGAAACTCTGACTTTATCTTCGATATGACCTGTAGCTT10.5747126436781609No Hit
GTGTGGTGGCACACACCTGTAATCCCAACTACTCAGGAGGCTGAGGCAGG10.5747126436781609No Hit
TCAGAGGGTGCAAGACAAGCTTGACGAGACCAACAAGAAGATCGACAAAA10.5747126436781609No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCGACAGAATCTGA10.5747126436781609Illumina Single End PCR Primer 1 (100% over 30bp)
ATCACAACTGTGCGTAGCATTTATTTACTGAAGTCCATATCCTTGGTTTC10.5747126436781609No Hit
GACCATGCTCAAGGACATCGCCAAAGCCGATCTGATCATCCTGGACGAGT10.5747126436781609No Hit
ATATAATCCGCTGGCAAATAAGCTTCCAAATCAAGATTAAGTTCAGCATC10.5747126436781609No Hit
AGCCATGAGCGTCCAATAGTTCAGGAGCATAAAGTAAAGATTCGTAAATT10.5747126436781609No Hit
CTGTTGCTCATTTTAGGGTGTATAGTAACTTCCCCAGCCTTTATCCACTA10.5747126436781609No Hit
GGAAAAGAACGAGGGGCGACTGAGCTTAGCTCAATCGCCCCTTCCGTCTT10.5747126436781609No Hit
GTCACGTCCTTCATCGGCTTCTAGTGCCAAGGCATCCACCGTGCGCCCTT10.5747126436781609No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA10.5747126436781609Clontech Universal Primer Mix Long (95% over 21bp)
ATCTTAACCGTTCCAAATCAAGTGAGATTTACGGAGATAAGACGGAAATC10.5747126436781609No Hit
ACACTCTTTCCCTACACGACGCTCGTTTTTCAGGACCTGTTGGTACCAGA10.5747126436781609Illumina Single End PCR Primer 1 (96% over 26bp)
GGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCT10.5747126436781609No Hit
TAGTTGAGCTGATCACGGCGTTGTTTGAAATCAAACGACGACACTTCCAG10.5747126436781609No Hit
CCAAACACTGATAACAAGTCGGTTGATTGACTACGTAAGATCATCGGTCA10.5747126436781609No Hit
ACATAGGATTCCTGCTTAGACTTACCCCTTTCTCTGGGCCCAGTAACTTC10.5747126436781609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5747126436781609No Hit
GTGTTGTTGCGCCAACGTGTTGGTTAATTGTGGCAAATCAAGGTCTGCCA10.5747126436781609No Hit
GGGCGATCGCGCGTTGAGACAGTGTGTTGCGCAGCCGAATCTCAATCTCC10.5747126436781609No Hit
TTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGA10.5747126436781609No Hit
CCCTGGCGGAGACCTAGCCCAGCGGGGTAAGGAGGGTGGGGGAAAACTGG10.5747126436781609No Hit
TCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTT10.5747126436781609No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTAT10.5747126436781609No Hit
CTATTGTATAATCACTATTTTCAATAACCTGTGTAACAAAGGCATCAATT10.5747126436781609No Hit
ACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATTGTGAAGATGATAAG10.5747126436781609No Hit
CTGCAAAGGTTGGCCCAAATCCTGCTTTAACCGGTTCATCACCGTGTATA10.5747126436781609No Hit
ACGCAGAGTACATGGGTGATACGGCGACCACCGAGATCTAAACACTTTCC10.5747126436781609Illumina Single End PCR Primer 1 (96% over 27bp)
TTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAG10.5747126436781609No Hit
GTGTTGAAGTGGCTTACAAAGCCGTTATGAAACCTGTTGAAGGAACGATT10.5747126436781609No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.5747126436781609Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT10.5747126436781609No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAAGC10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
CTACACTCTTTCCCTACACGACGCTCTTCCTGTCCCCTGTCTCTTATACA10.5747126436781609Illumina Single End PCR Primer 1 (96% over 30bp)
GGGAAAGAGTGTAGATCTCGGGCGCCCCATGTACTCTGCGTTGATACCAC10.5747126436781609Illumina Single End PCR Primer 1 (95% over 21bp)
CGCTTTAAGTAAATTTTTAGTGCGCCTGGCTTAACAGCAACGAATTGTCC10.5747126436781609No Hit
ATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCGGTACCGACAGA10.5747126436781609Illumina Single End PCR Primer 1 (100% over 37bp)
ATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACA10.5747126436781609No Hit
TATCTTAGCTGGGAACAAAACCTCCCAGCTATTTTTGATATTATTAATGC10.5747126436781609No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)