FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52.8735632183908044Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG42.2988505747126435Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT31.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GTTTCACTAATCTGTTTCATTTCCATACTAAATTACCTCGCACGAGTAGT21.1494252873563218No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21.1494252873563218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAAACGGACAGTCATCGGTGATATTTTGGTAACAGATGGCTACGCTCAGG21.1494252873563218No Hit
AACTCGATCCATGTGAAATAGATCATTTGAATAGCCCCAAAGCTATTAGT21.1494252873563218No Hit
GTTTAATGCTTATCGCGGCCGGAAATCAAAGGACTAAAAGTTGAAAGGAG21.1494252873563218No Hit
TTAGTAGGCGGCTTGATGGTAAGAAAGGCAGTCGGACCATTTTGAAAGTA21.1494252873563218No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGAT21.1494252873563218Clontech Universal Primer Mix Long (96% over 26bp)
CCACTCAGCCGTTGAAATCGTAACAAACGGTCTTGCACAAGCCAAAGAAA21.1494252873563218No Hit
ACATGGGAAATACGCGACACCGAGATCTACACTCTTTCCCTACACGACGC21.1494252873563218Illumina Single End PCR Primer 1 (97% over 34bp)
CCTGTAATCCAAGCCACTTGGGAGGCTGAGGCATGAGAATCACTTGAACC21.1494252873563218No Hit
GTGTAGGGAAAGAGTGTAGACTCGGGGTCGCCTATCACCCATGTACTCTG21.1494252873563218No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCTGTCTCTTATAC21.1494252873563218Illumina Single End PCR Primer 1 (97% over 37bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.1494252873563218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGGTATAACGCAGAGTACATGGGAAG10.5747126436781609No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA10.5747126436781609No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAACCACGAGATTACCCTT10.5747126436781609Clontech Universal Primer Mix Long (96% over 25bp)
GTAAATTGATTTGTAGTTTTTCATCTATGCCAAAGGAGGGAGTGGGTGTT10.5747126436781609No Hit
TTACATAATTATCATGCACATTGACGAAATGGCTGGCTTCAGACAAGGCC10.5747126436781609No Hit
GGATTTAGGATAGATTTGATAGATAGTTGCTTTGTGCCACCAATGTTTTT10.5747126436781609No Hit
TTCTTTTGTCCTTTTAACATGACCCCACTATTTGTAGGGGTTTCCTTGCC10.5747126436781609No Hit
GTACATGGGAATGTAGGCGACACCGAGACTACACTCTTTCCCTACACGAC10.5747126436781609Illumina Single End PCR Primer 1 (95% over 23bp)
TTACAGGTGTGAGTCACTGTACCCAGCCTTATCGTTTAGATTTTAATCTC10.5747126436781609No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.5747126436781609No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.5747126436781609No Hit
GTATTCTTTTTTAGTTTTTAAAACTAAAGAGTAGCTTTGGTCATGTAATC10.5747126436781609No Hit
CCGTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GCTCCTTCCATAACTACTTGGTGCATCAGCAAGTTCGGTTTAATGTCAGC10.5747126436781609No Hit
GCACAAAATGGAAACACAAAACACGGAGACAGATGGACATTTGTGGAATT10.5747126436781609No Hit
TACAATATTAGCATGGATCGTCTGTTCTAATATGCTGCAAGCAGGTAAAC10.5747126436781609No Hit
GCTTCGACCAGGTCGAGAAGCTCAACTGACATTCGTGATGAGAAAACCCC10.5747126436781609No Hit
GTACATGGGATGATACGCGACCACCGAGATCTACACCTGTCTCTTATACA10.5747126436781609Illumina Single End PCR Primer 1 (95% over 21bp)
GTGGTGGACGATGCGGTCGATGATCGAGGCGGCGAGTTTGTCGTCGGCGA10.5747126436781609No Hit
GTGCAAAGAAGATCGTTGCGCCCGCCTTTTTAGCAGCAACGATTTTTTTA10.5747126436781609No Hit
GTTAACATAGGTTAACAGCCAGATAGGAAGAGCACACGTCTGCCCATGTA10.5747126436781609Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAACTGACGAAACTGAAGTCAAGTTGGTGTTTCGGTTTTCGTCAGGTATT10.5747126436781609No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAATGAGGTTAAA10.5747126436781609No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAATGATACGG10.5747126436781609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGACAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTGTCTCTTATACA10.5747126436781609Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTCAGTCCCCGCATCATGGTCT10.5747126436781609Illumina Single End PCR Primer 1 (100% over 30bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GAGCAAGACTCCGTCTCAAAAAAAAAAAAAATGTGGTATCAACGCAGTGT10.5747126436781609No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGACGG10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
TAGTAGAGATGTGGTCTTCACCATGTTGCCTGGGCCGGTCTTGCACTCCT10.5747126436781609No Hit
GTGTAGGAAAGTGTAACCGTCGCTTTCCCATGTACTCTGCGTTGATACCA10.5747126436781609No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATGTACGG10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.5747126436781609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCA10.5747126436781609No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA10.5747126436781609No Hit
CGCTCATAAGGGGATGGCCATGGCCGTACGCCTAACCGCTAACATTACTG10.5747126436781609No Hit
TCACAGAACCTTTGCTGCTACTGCTGGGAAATAGTATGGCTTTTCACCTC10.5747126436781609No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATGATACGGC10.5747126436781609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTAGGGAAATGTAGTCTCGGTGCATCCATGTACTCTGCGTTGATACCA10.5747126436781609No Hit
TATTGATTCTGTTGGATATGATTTGTATACACAGATGTTAAATGATGCTG10.5747126436781609No Hit
TACAAGACCTGCGTTTGCTGGGGATTTAGCTGGTAAAAAGCTCGTGGTGA10.5747126436781609No Hit
GTACATGGGAGACGCGACCCCGAGACTAACTCTTTCCCTACACGACGCTC10.5747126436781609Illumina Single End PCR Primer 1 (95% over 23bp)
GTTAATCAATGTTAATGTAGAGATCGGAAGAGCACCCATGTACTCTGCGT10.5747126436781609No Hit
CGGTGAATCCGTCGCGTCGATCGTGACGATGACCGGGCTTACGAGTACCG10.5747126436781609No Hit
GAATGAACAGCTTCAACGTATTTATGGTACGGCTTGGGCAGACAAAAAAG10.5747126436781609No Hit
AATGAGCCATTCGCAGTTTCACTGTACCGGCCGTGCGTACCTGTCTCTTA10.5747126436781609No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGG10.5747126436781609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTGACAACATTCTAGTATCTGGTGCCTTTGGTGAGTTGCTAGGTTGGGA10.5747126436781609No Hit
ACTTTGGCCATTCTGTTGAAACCATGGTTCGGGCAATGACTAACGTTAGC10.5747126436781609No Hit
GACATCATCAACAAGGGCGTCGTAACAGCGGATACCGGATATGTCACCAC10.5747126436781609No Hit
GTCCCAATCGTCTGCCCCAAGACAACTCGAGTTTCACTCCCCGGTTCACC10.5747126436781609No Hit
AGTCGAAATCATTCGTTTTGTTTAAACTATATACCAATTCGGTTCAGTCT10.5747126436781609No Hit
CGCTTCTCGCGGCTGGCGATCCAGGCCAGCAGGATCACCGTCGCGACGGT10.5747126436781609No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTT10.5747126436781609No Hit
GACTTCTGCTCCTGCCCCGTGATCTTGATCCTGGAGTCCAGAATTGCAAG10.5747126436781609No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5747126436781609No Hit
ATGATAAGGACACTAAATCATTCTTTGGAGGAGAATAATAGTGAACCATT10.5747126436781609No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACAATGCGTTTATACC10.5747126436781609No Hit
GAGTACATGGGAATGATACGGCGACCACCGAGTCTCACTCTTTCCCTACA10.5747126436781609Illumina Single End PCR Primer 1 (95% over 21bp)
GTACCCATCCCATCTTGTGTTGCCCATAACTCATGAAATGTAAAAAAAGA10.5747126436781609No Hit
ACGAGGGGCTGCTGACCCGCCGCGATCCCGTCGACTTCCAGCTCCAGACC10.5747126436781609No Hit
GTTTTAACACCCAGTTTTCGGTTTTAAACAGCGCTTCTCGAAAATCAGCA10.5747126436781609No Hit
ACGCAAAAAAAAAAAATAAAAAAAAAAAAAAATTACACTGCGTTGATACC10.5747126436781609No Hit
CCTCGGCGGCGATCGCGCTGGAGTCGGCCACGCGGTCCTGGCTCTGTCTC10.5747126436781609No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTTGATCCGAC10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GAGAGCAAGTAGGATGTGTATATACTGAAACAATCGGCTATCCACCACAA10.5747126436781609No Hit
CTGATGCACCAGTCCCGGGTGTTCTTCATCCAGTTCATATAGTTTTTGGT10.5747126436781609No Hit
CCCGTGAATACCTGATTTGATTCCATATATAGCCGCGAAATCTAGAATTT10.5747126436781609No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.5747126436781609Clontech SMART CDS Primer II A (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCG10.5747126436781609No Hit
CCCTTTGTCACCGAACCCAGCCCGTTGTCCCCTTCGCGCTCACCAGAACC10.5747126436781609No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGTTATGGCACCAGTAACTCCT10.5747126436781609Clontech Universal Primer Mix Long (96% over 25bp)
GTTCAAGACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAAT10.5747126436781609No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCCTGACTTGAC10.5747126436781609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATTAACGTCACTCACGACAAGCTATCCCGACTATCAGTCATTTCGCTT10.5747126436781609No Hit
GTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGGATCCGAGTTAC10.5747126436781609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGCGTCGTGTAGGG10.5747126436781609No Hit
GACCTGATCACGCCGACCGACATGGTCGTGACGCTCAGCCACACCGGCTA10.5747126436781609No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTGAATTAGTCTATTTAACAAGTTGACCAAAATATCGACCTCTTCCT10.5747126436781609No Hit
GTACATGGGATGAACCAACGAGATTACCTCTTCCGATCTCTGGTACCTGC10.5747126436781609No Hit
CGCATCCTTGCCTTCATTTTCAATACCTGCATAAGGATGAATATCTCTTT10.5747126436781609No Hit
GCCTTTTAACTGTATTTCTCAGATATGACATCTGATGGCCCAGCCTTTTA10.5747126436781609No Hit
TTGCAGCACCACGAGAAACTGTCAAAATCGTTCCTTCAACAGGTTTCATA10.5747126436781609No Hit
GTATCAACGCAGAGTACATGGGGATTACGCGCCACGAGTCTACACTCTTT10.5747126436781609No Hit
AATCTTGCATAATTCCCGCGCATTCAGCTTCTAGAATCCCTGTTTCCACC10.5747126436781609No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGTATCAAC10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GAATGAACAGCTTCGACGTATTTATGGTACGGCTTGGGCAGACAAAAAAG10.5747126436781609No Hit
GCTTAACGCGTTCCCGGATGCGATCGGCGCATACCTGACGCCGATCGCAT10.5747126436781609No Hit
GTAGAACTAGACACTCTTGACGGCTGCTGTACTACTCTCTTAGACGAACT10.5747126436781609No Hit
GTTAATAAGGGCGCACGGTGGATGCCTTGGCACTAGAAGCCGATGAAGGA10.5747126436781609No Hit
AACCCCTTCTCTACTAAAAATATAAAAATTAGCTGGGTGTGGTGGTGCTG10.5747126436781609No Hit
CTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
AAACCAACCAGTTTTTAGGAGGCGTGCTGATGCAAAGTGATGAGCATTTA10.5747126436781609No Hit
CCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GTATTGGAGTCTCACAACTCTGGTACCATGTGCAAGGTTAAAACAAGAGG10.5747126436781609No Hit
GTATCAACGCAGAGTACATGGGTGCGACCCAGCTCACCTTCCTACACGAC10.5747126436781609No Hit
ATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATGTACGAGGAAGT10.5747126436781609No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCTACACGACGCTCTTCCGATCTCAGTCCCCGCATCATGGTCTGAGA10.5747126436781609Illumina Single End PCR Primer 1 (100% over 26bp)
CCTACCTCCCATTCCCCTATTTTGATTTAAAACATCACTTATTAGTTGTA10.5747126436781609No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTTGATACGGCGA10.5747126436781609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACTTGTGGCCGCCCTGTACAAGAGAGAACCAGACTCCAATGTCATTGTG10.5747126436781609No Hit
CATTCCTTCAGACCCAGTTTTCCCCCACCCTCCTTACCCCGCTGGGCTAG10.5747126436781609No Hit
CCTCAACTTGGGGACGGTCAAATCCGCCGACGAGCAGGCGCTCTCGACGC10.5747126436781609No Hit
GAATGATACGGCGACCATCGAGATCTACACCTGTCTCTTATACACATCTG10.5747126436781609Illumina Single End PCR Primer 1 (96% over 29bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATCGGCG10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
CCCGCGTCCAGCCTCGAGACGACCTCGAGATGCTCGGCCTGTCTCTTATA10.5747126436781609No Hit
GGTCGAACTGGAGCAGGAGCCGGTGGGTATCGTCATTTCTCGTGGGAGCC10.5747126436781609No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
AAATCAACCTGCTTGAAATCTGCCAATTTGGCTTGATTGGCTTCGTTCCA10.5747126436781609No Hit
GTACATGGGAGGACACCGAGTCCACTCTTTCCCTACACGACGCTCTTCCG10.5747126436781609Illumina Single End Sequencing Primer (96% over 29bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.5747126436781609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGTGAGATACCACGACGAATATTTGTATTCGCTAATGGTGTTTCTTCA10.5747126436781609No Hit
GCTAACGGTAACAAAAAGATGGCTAGCATTTGTAACGTCAGTACCGGAGT10.5747126436781609No Hit
GTAGAAGCTACAGGTCATATCGAAGATAAAGTCAGAGTTTCTGTACTACA10.5747126436781609No Hit
CCATACCCTGTGTCTGGAAGGGATTTTGGTGGTATTCACATCCCAGTGCT10.5747126436781609No Hit
CTACTGATCCAGCCACTGGATATTTTATATCCTCCCTTTTCCTTAAGCAC10.5747126436781609No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTC10.5747126436781609Clontech Universal Primer Mix Long (100% over 23bp)
TACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCAT10.5747126436781609No Hit
GGTCAACCTGACGTGGCCCTTTATGGTGGCCGGGAAACCCGAGAGTGACC10.5747126436781609No Hit
GCGAGCATCCTCGAAGTTTTTGTCGATCTTCTTGTTGGTCTCGTCAAGCT10.5747126436781609No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGTCTCTTAT10.5747126436781609No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACG10.5747126436781609Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAA10.5747126436781609No Hit
GATGGCCGCGTTAGTGGAACGGTTAGCGCGCCACCAAACGCCGCAGTCCG10.5747126436781609No Hit
GCTTGCGATACGGTGGACACGTGGCTGGATGACACGGCCAAGGGCAGGCA10.5747126436781609No Hit
CTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTC10.5747126436781609No Hit
CAGACAAGCTTCGGAAGGAGCGCCGAGAGTTCTACATCCCCCAAGCAAAG10.5747126436781609No Hit
TATCTGATTCATCCACAACGTCGTCGTGTAGCAGAGTCGCCGTGTGGATA10.5747126436781609No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)