FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGCAACCGTGGGGCGGCCGTGCTCGGCTGGCCGAGGCCCGGCCCGGCG23.4482758620689653No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC23.4482758620689653No Hit
GTCGGCGGCAACGCCGAATGGGTCGAGCAACTGATTGCAGCGGCCACGCT23.4482758620689653No Hit
CAGTCCAAAAAAGCACTTGCAGGCTTTACAAACAGAGTGTTTCCAAACTG23.4482758620689653No Hit
ACTCTACTCAGCAGCTGTTTGCTATGATTGGAAGACATTCAAACTGGGTA11.7241379310344827No Hit
GTCCTCACCCAAATCTCATCTCAAATTGTAATCCCCATGTGTCAAGGGAG11.7241379310344827No Hit
TGGCAGCAGTGACTCTCCAAAACTGCCGAGGCCTAGACCCCCTCACTGCT11.7241379310344827No Hit
AATCTCAGCTGTGCATCTCCCCAGCTAGCCTTTTCAATGGGAAGATACCA11.7241379310344827No Hit
TGACACGCTGGCGTTCGTCATCGTTCATTGCATCCACCGCCTTGATCAGT11.7241379310344827No Hit
TACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACC11.7241379310344827No Hit
CGATGAAGTTGATCAGCAAGAGCGGATTGGCCACCGAGAGCCACACCGCC11.7241379310344827No Hit
ACATGGCCAAGCCCAACATCAGTGGAGTGGAAGAATACCTGTATCTTATA11.7241379310344827No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC11.7241379310344827No Hit
ACAGAACAATAACCTGAGCAAAGCTGGTACTTGGACTAGTTCTGTCTCCA11.7241379310344827No Hit
GGCTTCATCAACAGCGACCGCGGCGGGTACCATTCCGCTCTCAACTGCGG11.7241379310344827No Hit
GTCCTTTTCCTGGCTCATCCTGGCTCAAAAACACCCCCACTGAGCACCTT11.7241379310344827No Hit
CACCTGTAATCCCAAAACTCTGGGAGGCCAAGGCGGGCGGATCGTGAGGT11.7241379310344827No Hit
CCTGGGCTCAAGCAATCCTCCACCATAGCTGGGACTACAGGTGCATGTCA11.7241379310344827No Hit
GAAGTTCATCAAGCAGCGCGAAAGTGCCAAGGTCGAGGTGCCGAAGCAGC11.7241379310344827No Hit
GTGCAGGAGCGCTACGAGGCCTACACGCAGGAGGCCATCGACATCCAGGT11.7241379310344827No Hit
GAGTGGCCATGAGCGCGACCAAGCAGGCGCTCCAGCGCGCTGCCCAGGCA11.7241379310344827No Hit
GTTGCGGCCAGAACCTGCGGCACGGCATAGACCGTCAAGCCAGCCAGAAC11.7241379310344827No Hit
AGGCCGTTGTCGTCCGCGACGTAGGTGATGTCGCCCTGCGCACGCGCCTG11.7241379310344827No Hit
GAATCGAATAGAATCATCGAATGAACTCAAATGGAATCATCATCGAATGG11.7241379310344827No Hit
GGTAGAGGGACAGGGGGCCGCACTGCAATCAGCGTTGGCTTCACCTTCAG11.7241379310344827No Hit
GCTGGGAGGCCTTGGGAACCGGGCTCATGGCGCCGAGGTCGGCGCGCCAC11.7241379310344827No Hit
GTGGTTAGTCTCCTGTTTTTTGTTTTGTGTTTGTTTGTTTGTTTGTTTTG11.7241379310344827No Hit
CATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGG11.7241379310344827No Hit
GTCGACGGAGTAGCGGGCGAAGCCGCCGCCGAGCTGGTCGTAGATGCCGC11.7241379310344827No Hit
GTCGGTCGCCTTGCATGCCCTGGAGGACTCCGGCTTTTACCTGTCTCTTA11.7241379310344827No Hit
TTCTTATATCTTTCCCTTTTTACTTTGCCTAACTAAATGCTAGCAAAACT11.7241379310344827No Hit
TCTCAACGTGGCGGGCATGGTGGCGTCGGTGCTCCATCCTCCACCATGAA11.7241379310344827No Hit
CTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAA11.7241379310344827No Hit
GAATACATCTCCCCATCCACCCAAAAGAACGCCCCCAAGCTGGGCATCTT11.7241379310344827No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG11.7241379310344827No Hit
AATGCGGCCCGCGCTCAGCCCGCTTGAGTTCTGCGCCTCCCGAGCGGGTC11.7241379310344827No Hit
GGACTAGAATGGAATCATCATTGAATGGAATCGAATGGAATCAACGAATG11.7241379310344827No Hit
AGGTCGGGAATTCTCCGGCCTGGAAGCCATAGTCCTCTTCGTTGGCGGTG11.7241379310344827No Hit
GTCCAGAAGTAAACCTCTACATTTATAGGTAACTAATTTTCTTTTGTGTG11.7241379310344827No Hit
ATTATAAGCTGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGA11.7241379310344827No Hit
ATCCGCGCGTGCTGGCGGCCATGCAGGAGCAGGCGGCGCGCGTCACCTTC11.7241379310344827No Hit
GTCCGTTGGGGACCCAGGCTCTGCTTCTCCGTGGTGCGGTTTTTTTTTTT11.7241379310344827No Hit
GAAGTGATTGGATCATGGGGGTGGATTTCCCCCTTGCTATTCTTGTGATA11.7241379310344827No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTAGGCCTTAAAAGTTGTCGTTTGA11.7241379310344827No Hit
AACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGC11.7241379310344827No Hit
GATTCGCACGACGCTCTCGGCATTGTGGTCGTGGGCGGTGCGAGAGAGGT11.7241379310344827No Hit
ACCTCAGCCTCCCGAGTAGCTGGGATTACAGATGTCCACCAACACCTTGG11.7241379310344827No Hit
GTTTGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTATTAAAAAT11.7241379310344827No Hit
GTCCAGCACGTCGTTCTCGTTGGTGGCCACCACCAGCCGCCTGTCTCTTA11.7241379310344827No Hit
AAAGCACCCCCGTCTCGGGGATGAGGTTGTTGAATGGCGCCATGTTGACG11.7241379310344827No Hit
GTTCGTGCCTGACGAGATCTCCGGCATCGTCGTGTCGGGCATCGCGATCG11.7241379310344827No Hit
CTCGTAGACGGCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACC11.7241379310344827No Hit
CGCGTCACCCGTTCGCCGGGTCGGGGCCTCAGGTAAGGGAACGGTAAAGC11.7241379310344827No Hit
GTGGCGAATGCGGTGCTCGGCGAGGTGATCGACCAAGATCTCGTGAGGCG11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)