FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG63.488372093023256Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG52.9069767441860463Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGATCGAGATCTTGATGAAGCGCGAGCTGTCGTAGGGGATGGCGTACTT31.744186046511628No Hit
GGAGAAACAATTTTGAGAACCAGGAAAAAAGAGAGAAAGAGGTAGAGTTG21.1627906976744187No Hit
GTGTAGATCTCGGTGGTCGCCGATCATTCCCATGTACTCCTGTCTCTTAT21.1627906976744187Illumina Single End PCR Primer 1 (95% over 22bp)
ATTATTCCCTGCTCTGAGTTAGTTACCTTCTGAGGAGACGAATGCTCTCC21.1627906976744187No Hit
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTC21.1627906976744187Illumina Single End PCR Primer 1 (100% over 35bp)
GGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCGCAGGCTCCACTCCTG21.1627906976744187No Hit
TCCCTACACGACGCTCTTCCGATCTCTGGTACCGACAGAATCTGAGGCAA21.1627906976744187Illumina Single End PCR Primer 1 (100% over 25bp)
GGTTTCCTGTTGGTGATCCACGGCGCAACCGACAAATTCGCGCCGGCAGG21.1627906976744187No Hit
GTACTCAGGAGCTCTCATCCTCATGCCTTCCCTCAGGCGACTGCAAAAGT21.1627906976744187No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA21.1627906976744187Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGAAACACAAGCTTTAATCACGCGAAAGACACAGACACATGAAAGCCCT21.1627906976744187No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21.1627906976744187Clontech Universal Primer Mix Long (96% over 26bp)
CTGTCAACCAGGCTGGAGTGCAGTGCAGTGGTGAGATCACAGCTCACTGC21.1627906976744187No Hit
GTTTAACAGTTTCAAATGACCCTCCACGGTTGAGCCGTGGGCTTTCACAT21.1627906976744187No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA21.1627906976744187Clontech Universal Primer Mix Long (96% over 26bp)
GCCTTGACCCGCTCGTCACAGACGGATTTCTCGATCGTGTCGCCACCCTG21.1627906976744187No Hit
CATGGTGATTACTATTCAGTTTTGCTAAGTGCAACCATAGGAGTAGGCAT10.5813953488372093No Hit
ATCATCTAGTCAGACAGGAACAGCACCACGATCATCTAGTCAGACAGGAA10.5813953488372093No Hit
GTGCTGGGCCACATCGAACAGACCTCACCGAGCCTGCTGGTGGTGGACTC10.5813953488372093No Hit
GTGTATATGTGCCACATTTTCTTTATGCAGTTTATTATTGATGGACATTT10.5813953488372093No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10.5813953488372093No Hit
GATTCGTCAGGGCCTGGTGCGTTCCCGTATCCGCGGTCGTGCGCTCACTC10.5813953488372093No Hit
GGTAACAATGGGACAAAATGAAAGTAAACATTCTGCTTGTTTAAACTTTC10.5813953488372093No Hit
CTTTTTGCTTATGCAAAAATCTGGCAATTATCCTTGATTCGTTGATTTCA10.5813953488372093No Hit
GTATCATTTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.5813953488372093Clontech Universal Primer Mix Long (96% over 26bp)
CTTGAATGCAAACATCACAAAGAAGTTCCTGATAATGCTTCTGTCTACAA10.5813953488372093No Hit
ATGTATAACTGTATTCCTTGGTATCACATGGTGCATTGGCCAGCCTACTA10.5813953488372093No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.5813953488372093Clontech SMARTer II A Oligonucleotide (95% over 21bp)
ACATGGCTGATAGGCGCCCCGAGATCTACACTCTCGACGCTCTTCCGGTC10.5813953488372093No Hit
AGCCAGGACTTGGCTTGTTCGATCTGGGGACCGGCAGCGACAGGCACAAC10.5813953488372093No Hit
TCCCTACACGACGCTCTTCCGATCTGGGCCTCCAGTTCCTGAATGAGGAA10.5813953488372093Illumina Single End PCR Primer 1 (100% over 25bp)
CCGCAAACTCCTTTCGTTCGCTTTCAAAAAGACATTCCCGCGTGACCGAC10.5813953488372093No Hit
ATGTGTTAACAAAGCACTTCTTAAAATGGTATTTTTTAAAAGTTCAAATA10.5813953488372093No Hit
AGGTTGAAAGATATAAAATCAATACACAAAATATAAAATATAGCATACCA10.5813953488372093No Hit
GCATGCCAGCTGTGGCTTGGACAGACTATTTATTGGATCTCTCTTTGCCT10.5813953488372093No Hit
GACCCGGGGTCCCCGGCGGGCGTGCGCGGCGGGTGGTCGCCCCGTTACGA10.5813953488372093No Hit
TCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTTCTGGTATCGAC10.5813953488372093Illumina Single End PCR Primer 1 (100% over 36bp)
GTGTCCTCTCCTACGTGGTTGACGGCTCCTGAGACCACCTCGGTGACGAT10.5813953488372093No Hit
CTATAAGACAGAACAAAGAAATCCTGCAGTCCTGTCGGCTTTTAGTGAGT10.5813953488372093No Hit
ACCGAGATCTACACTCTTTCCCTACACGACGCTGTCCCTGTCTCTTATAC10.5813953488372093Illumina Single End PCR Primer 1 (97% over 35bp)
CATGTACTCTGCGTTGATACCACTGCTCCCATGTACCTGTCTCTTATACA10.5813953488372093Clontech SMART CDS Primer II A (96% over 25bp)
GGCGTAGGCAAGCGTTTTCAATTTTTCCGCAGTCGTTTACGCCAGTCCGG10.5813953488372093No Hit
TCCCTACACGACGCTCTTCCGATCTCTGGTACCGACAGAATCTGAGGCAC10.5813953488372093Illumina Single End PCR Primer 1 (100% over 25bp)
ATAGTAACCTGCATCGCATCTCAGGTTGCCCGATCCCGTGGTTGTGCCCG10.5813953488372093No Hit
CATGTGGTGTTATTTGGAGATGGGACCTTTCTGTCTCTTATACACATCTC10.5813953488372093No Hit
GAAGAAGATCGCCAGGATCACGTCGGAGTCGAATTCGATCATGGTCTTCA10.5813953488372093No Hit
GACCAGCATGGTGAAACCCTGTCTCTACTAAAAATACAAAAAAAGAAAAG10.5813953488372093No Hit
CAGTTCGGCGGGGCTGGAAGAGTTTGCTGGGTATCCTGGAGGGTCCAGCC10.5813953488372093No Hit
GGCTTGTGTAAGAATTCCTGGTCTAGGTTCTTTCCTGACAGTTCACTTGG10.5813953488372093No Hit
ATGGAATATTATTTAGCTTTAAAAAAGGAATGAAATTCTGATAAATACTA10.5813953488372093No Hit
CTACTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCTGTCTCTTATAC10.5813953488372093No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.5813953488372093Clontech Universal Primer Mix Long (95% over 22bp)
CGCGCGAGCTTCCCCAAGCCGCACGAGCTCGACCAAACCGTCGAACCGGC10.5813953488372093No Hit
CGCGGCGCTCGACCAGTGGCCCCAGGCGACGGGCATTAGGGTCGACCGGT10.5813953488372093No Hit
TTATGGGGCAGGAACACTGAGCTTGGGGAATCCCTGTCTTTGTAGAGAAC10.5813953488372093No Hit
GGTCTATATATCATTCTGATATGTCCAAGAAGATCGCTAAAGGCATCACG10.5813953488372093No Hit
CATAAAGGCTGAGTTGCACTGCATGTCCATTAGGATGTTTTTCACATCCC10.5813953488372093No Hit
CCCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTCTGTC10.5813953488372093Clontech SMART CDS Primer II A (96% over 25bp)
GTGTATCTTCGAGGGGTTGCTGACGCAACGACCCTCCTGCCACGGATAGT10.5813953488372093No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCCAGAACAGATCGGAAGAGCGT10.5813953488372093Illumina Single End PCR Primer 1 (96% over 29bp)
GAGGTGGAGGTTGCTGTGAGCCAAGATCGCACCACTGCACTCCAAGCCTG10.5813953488372093No Hit
CGCGATCACCGTCGTGGTCATCACCTGCCCCGACGCGTTGGGTCTGGCCA10.5813953488372093No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCT10.5813953488372093Clontech SMART CDS Primer II A (96% over 25bp)
CTTTTCCACTTAGCAAATATCGACTTTGACTCTTGTCTGTGAGTGGAGCT10.5813953488372093No Hit
GGTGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGCTGTCT10.5813953488372093No Hit
GAATGGAAGGGAATGAAATTGAATCAACAGGAATGGAAGGGAATAGAATA10.5813953488372093No Hit
ACCCTCCATTTCAGAAGATGCTCTCTGACGTTCAAACCGCCTCGTGTGTC10.5813953488372093No Hit
CCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCAGATCACGAGGTCA10.5813953488372093No Hit
ATCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.5813953488372093Clontech Universal Primer Mix Long (96% over 26bp)
GTGACCTCTCCTACGTGGTTGACGGCACCTGAGACCACCTCGTAGACGAT10.5813953488372093No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.5813953488372093Clontech Universal Primer Mix Long (96% over 26bp)
GTGTTAAGAAAGAGAAGACTCGGCCAGGTGTGGTGGCTCATGCCTGTAAT10.5813953488372093No Hit
TATTACTGCTTTCCAGAGGCAAGTGCTTATAACTTTTAGCTCTTTCTTGT10.5813953488372093No Hit
GCGCGATTCCGAGCAGTCAGGCAGCGCGTCCACGTCGCCGGGGGTTACGT10.5813953488372093No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCGGATCGATCGGCGACGGCGTCGCGCTCGGCGTGCCCGTCGGCGACG10.5813953488372093No Hit
GGGCAAAGGTCGGAGACGTCTGGGCCGCTGCAGGGCCGGTTGCCGTGGAG10.5813953488372093No Hit
GTGTGTAAGCATTCCCTTTTCTCCACAACCTTGCCAGCATCTGTCATTTT10.5813953488372093No Hit
TTCTTAGATCCATCAGAGAGGAGTGAGGTCACAGGGCAAACTTCTGTTCC10.5813953488372093No Hit
TCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGA10.5813953488372093Illumina Single End PCR Primer 1 (100% over 36bp)
GTTCTCGGGCAAGGTCGCGGATTCGGGCGAGGGCCAGTGGACGATCGACG10.5813953488372093No Hit
CATGGTGATTACTATTCAGTTTTGCTAAGTGCAACCATGGGAGTAGGCAT10.5813953488372093No Hit
GTTTGAAGGTGTATATCAATTCGTTTCGCGAAGTGAAAATCCTGTCTCTT10.5813953488372093No Hit
AAATGTGAACTAGGCTTTAGGGTATGGGAGACAGAGAAGAGGAACAGAGG10.5813953488372093No Hit
GTATGGTGTTGGAGAAGCTGTTGGACAGGTAGATGTCGGGCGACCCGCCG10.5813953488372093No Hit
GGAAACAGTTCATCGATACCAGCGCAGTCTCTCCGGCAGCATCAACGATG10.5813953488372093No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGTTGATACCTGTCTC10.5813953488372093No Hit
CCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGTCGATACCAGAAC10.5813953488372093Illumina Single End PCR Primer 1 (100% over 24bp)
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT10.5813953488372093No Hit
ATGTTGGCCAGGCTGGGCTCGAACTCCTGACCTCATGATCCAACCGCCTC10.5813953488372093No Hit
TCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCGACAGA10.5813953488372093Illumina Single End PCR Primer 1 (100% over 36bp)
ACACGAAGCCGAGCGGCACGTTGCTCGATCCGCCGACGCATGCGCATTTG10.5813953488372093No Hit
ATGCACAACTCCACCCTGAAGCTGAGACTCCCCCAGCCACATCCTGCTTC10.5813953488372093No Hit
TGACAAGGCAGTGCAGTCTGTTCAAGACAGCCTGAAACACTGAAAGCTTA10.5813953488372093No Hit
GGAAGACGTCGTGTAGGGAAGAGTGTAACCGGCCCCATGTACTCTGCGTT10.5813953488372093No Hit
TGTAAAGATACTCCTTTTCCCATTTAACTTTTCATACTGAATTGGGCACT10.5813953488372093No Hit
GTCCCGCACCAGGCGAACGCGCGCATCCTCGACGCGACCGCGCGCAAGCT10.5813953488372093No Hit
CTCCAGTCTACTGCATCTCTCGGGAAATACCGAAAGATTTCCCGAAGTGT10.5813953488372093No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.5813953488372093Clontech SMART CDS Primer II A (96% over 26bp)
GCCATGGTGCAGCGTGTCAGACTGGCTGACCTGGCAAATCCGGCGGCTCG10.5813953488372093No Hit
TAGTAAAACGGTAGCATAATTTTCAGAAAAGTAGTGACTTATGCCTGTTT10.5813953488372093No Hit
GGTTCTCGATGACGGGCGGGGACGCGATGTCGGGCCTGGTCCGGCGGCAC10.5813953488372093No Hit
CCACCAGCCCACTTGGGCCACCTCACCTGAGACGAACCCTGTGGGTAAGC10.5813953488372093No Hit
CTGTAATTCATTAAAAATGTTTATTGATTTAATTCAGTAAATGTTGAGTA10.5813953488372093No Hit
GCACAGGAAGGCGATCCAGCTCGCGCGGCCGGGCAGCGCCGCCCACGCGG10.5813953488372093No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA10.5813953488372093Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGTACCGACC10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCATGTCTGGTACTTTTGAGGAATTGACCGTACCACCAACCTTGGTTG10.5813953488372093No Hit
CGGTTCGAGGCGGCGAGCCCGTTCGACTATCCCGCGCACGCGCAGGTGCT10.5813953488372093No Hit
AACAGATTGTATCCATTGAAGTAAATTTTTCTTCTCACTATTGCTTCTGC10.5813953488372093No Hit
TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGG10.5813953488372093No Hit
CGACCTGGTGTTCGCGCTCGACTCGATCCCCGCCATCTACGGCCTCACGA10.5813953488372093No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTA10.5813953488372093Clontech SMART CDS Primer II A (96% over 25bp)
GGCACGTAGACCCGTACCCGGCGACCCTGGGCGTGCAGCCGCCGCTGTTC10.5813953488372093No Hit
GTAGCGACCCGGCCAGCATGCGCACCTTCGCCCGCCGGGACGGGGCGGGG10.5813953488372093No Hit
GATTACAGGCATGCACCATCACGCCTGGCTAATTTTTGTATTTTTAATTG10.5813953488372093No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.5813953488372093Clontech Universal Primer Mix Long (96% over 26bp)
CGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACACCCATGTACT10.5813953488372093Illumina Single End PCR Primer 1 (97% over 38bp)
GGATAACTCGAGGTGAAGGCCAGGATGACCTGCCCCAGAGCCTGTCTCTT10.5813953488372093No Hit
GCCTGGCGGGGACCGTCTCCAGCCGGCGCACTCGCTTCCTGCTCGGGTGA10.5813953488372093No Hit
TCTCTGCCTGATAATCCAGAGAATTCCCCTGGAGCATGTCCAAAACCATC10.5813953488372093No Hit
CATGGGGCCGATACACTCTTCCCTACACGACGCTTGCCTCGATTCTGTCG10.5813953488372093No Hit
GCTTGGCAATCTGCATCTGTCGAACCGCGAGCTCTTTGCTGAACTGCGAA10.5813953488372093No Hit
ACCCTGGCTGGCCCACCGCCTCGACGCCGCCGCCGAGCTCTACACCAGCG10.5813953488372093No Hit
TATCTTAAATATGCAACAAGAAATTTTCCTCCATATTTTGTCTTTGCTTT10.5813953488372093No Hit
AAGTCATTATGGACACTGCAACGCAACCCTTGTTTTCAAGGATTCTATGA10.5813953488372093No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTCGCGGGGG10.5813953488372093Clontech Universal Primer Mix Long (96% over 26bp)
GTCTGGGTGTGGGGTCCCGACGGAGGGGGCCGGCGTCCCAGGACCCTGAC10.5813953488372093No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCAGACCATGATGCG10.5813953488372093Illumina Single End PCR Primer 1 (100% over 36bp)
CCACGCACGAGAGGGTGCGATCTCAAGGAGCAGATCACGTTCTACCGGAG10.5813953488372093No Hit
GAGTCCCCCGGGACAACTGGTCGACACCGCAGGCCTACGTTCACGGGACT10.5813953488372093No Hit
CACTAAGTTAGGGACTGTTTATGAACCAACTATTTACTACGTAATGCCAA10.5813953488372093No Hit
AGACTGAGTAAGTCTTGTCCAGTGGCACGTGAGGAGGCCAGGCCCAAGCT10.5813953488372093No Hit
GAACTCGACGATACACTCGTCTCGACGCAAGATGCCGCCATCATCTGAGT10.5813953488372093No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGCCGACCAAGTCCCCCTGGTTTATGAAGTTGCTTCTATTCCTGAAAAA10.5813953488372093No Hit
CCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACC10.5813953488372093No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTAT10.5813953488372093Clontech Universal Primer Mix Long (96% over 26bp)
TCTGTGAGTTCAAGGCACACATCATAAACTAGTTTCTGCGAATGACTCTG10.5813953488372093No Hit
ACATGGGTGAACGGGCACCGAGATCTACACTCTTCCCTGAGTTGCTGTAG10.5813953488372093No Hit
GATCTACACTCTTTCCCTACACGACGCTGTCCCAAGTTCTGCAGGTACCA10.5813953488372093Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCGTCGTGTAGGAAAGAGAGATCCGGCCCCATGTACTCTG10.5813953488372093Illumina Single End PCR Primer 1 (96% over 25bp)
AGAGTTTACAGATTATTTGTTCAACAAGTCACTAGAAATTGAACCTCGAA10.5813953488372093No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)