FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52.9069767441860463Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCACCGTTCGCCTGCGCGACGTGGCCCGCATCGAGTTGGGCGCGCAAAG31.744186046511628No Hit
GGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAA21.1627906976744187No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA21.1627906976744187Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGAACGCTTGAGCCCGGGAAGCGGAGGTTGCAGTGAGCTGTGATCTCA21.1627906976744187No Hit
CTCTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAG21.1627906976744187No Hit
GTATGGATTCGAAGAGGTCATCAAGTCCAACCCCCCTTGAATATCCCTTA21.1627906976744187No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC21.1627906976744187Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCGACCGCCGCACCTGCACCCGCACCCGCCGAGCGTGGATCACCGACTC21.1627906976744187No Hit
GTGCTCCTGTTGTCCCAGCATCATCACCAGTAGGAATCCCTGTCGCTCTC21.1627906976744187No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21.1627906976744187Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAATGATCGGCGACCACCGAGATCTACACCTGTCTCTTAT21.1627906976744187Illumina RNA PCR Primer (95% over 24bp)
GTCCGGGCGGGGGTCCAGAATTTCTGAAGGGCTTTCATGTGTCTGTGTCT21.1627906976744187No Hit
TTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGT21.1627906976744187No Hit
CCATAACACTTCGGGAAATCTTTCGGTATTTCCCGAGAGATGCAGTAGAC10.5813953488372093No Hit
GATGTCAGCCATTTTAGGTACATTATTTATACTGGGAAGGAAGTGCCAAA10.5813953488372093No Hit
CTGCCAGTGTGTAGGGGAGGAAATGAGGCAAAGAGAGATCCAAGAAATAG10.5813953488372093No Hit
GATCACCTGAGGTCAGGAGTTCGAGACTAGCCTGGCCAACATGGTGAAAA10.5813953488372093No Hit
CATAAGACCTCATCACCTCCTGTGGACTAGTGAAAACGGCCACGGACTAT10.5813953488372093No Hit
AAAAAAATGCTCATCACCAATCAGTAGAGATATGCAAAGAAAAACCACAG10.5813953488372093No Hit
CGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCACCCTGTCT10.5813953488372093No Hit
CCTATCACATACTTCTTGCCGTCATACTGTCAATGGTGGCAATTCCAAGT10.5813953488372093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG10.5813953488372093No Hit
GTTCAAATGATTCTCCTGCCTCAGACTCCCGAGTAGCTGGGATTACAGGT10.5813953488372093No Hit
CTTTAATGTCGTAGGAACCCTTTGCAGCATCAGGTAAAGCTTGAATACAA10.5813953488372093No Hit
GTACTTGGGATACGGCGACCACCGAGATCTACACACTTTCCCAACACGAC10.5813953488372093Illumina Single End PCR Primer 1 (96% over 27bp)
AGTCTCTACAGTGAAAACTAACATTTGAGCATGATTCTTTTTTTAAATCA10.5813953488372093No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTCTTAT10.5813953488372093Clontech Universal Primer Mix Long (96% over 26bp)
ACGCCGTCCAGGATCCAGTCGGCGCCGTCCCCCGCCCCGTCCCGGCGGGC10.5813953488372093No Hit
CAGAAACTATGTACTCGTTAAACAATAACCCCACCCCCCATTTCCTCCTC10.5813953488372093No Hit
GTACATGGGAGTACGGCCACCGAGACTAACTTTCCCTACACGACGCTCTT10.5813953488372093Illumina Single End Sequencing Primer (95% over 22bp)
ATATCACGTACATTTGTGTTAGTGGATTTTATTTCTGTTACTAGGTCATA10.5813953488372093No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.5813953488372093Clontech Universal Primer Mix Long (96% over 26bp)
GGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGTCTCTTATAC10.5813953488372093No Hit
CGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTC10.5813953488372093No Hit
AACTGAACACTTGTTCCCGAAATTATAAAAGACTTACAAATCGAGGTGGC10.5813953488372093No Hit
CCATACCGCGTTGCTTCGCGGTGCGCACCAACGCTCCAGTGACCTCGCGC10.5813953488372093No Hit
GGGCCATCACCCCGCTGGGCAACACGCTGATCGCGCTGACCAAGAACACC10.5813953488372093No Hit
AACAAGATGAATACACGAAAAAATGGGAATACGCTGAAGAAAGTCTAGGT10.5813953488372093No Hit
GTACTATTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTATAAAACCACAC10.5813953488372093No Hit
GTGCAGGGTTGAGGGGCAAAAGCTGTTCCCAAAGTTTCCCAGAAACCCAG10.5813953488372093No Hit
GTGCTTCATGTGTTACTGTCAGGCTTTGTAACTCACTAAAAGCCGACAGG10.5813953488372093No Hit
ACACGACGCTCTTCCGATCTCAGTCCCAAGTTCTGCAGGTACAAGATATC10.5813953488372093No Hit
GTCCAAGGCGATCGCCTGCTCGGGGATCCCCCGGGAGAGGAGGTAGCTGC10.5813953488372093No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.5813953488372093Clontech Universal Primer Mix Long (96% over 26bp)
AAAGGTCCCATCTCCAAATAACACCACATGCTGTCTCTTATACACATCTG10.5813953488372093No Hit
CTCCTGAATCTATCTTTGACAAAATCTACAGCACCAAGAGCGACGTGTGG10.5813953488372093No Hit
CACACTGTACTATTATCAATTTTCTCTTTCTTCCTTCCAACTTCTTCATT10.5813953488372093No Hit
GGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCG10.5813953488372093No Hit
ATATTAAGGCTATTTGCCAAAATGTTTTAAAATCCTGGTATTTAAAGCAC10.5813953488372093No Hit
GGCAGATAGAACGCTGCGGGGGAGAGGCCGGAAGCAACCACGCCCTCCAG10.5813953488372093No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTCTATCCAATATCCATCCAGGAAGCAGAAGGCCAGGGAGCAGGAGGGA10.5813953488372093No Hit
GGACACACGAGGCTGTTTGAACGTCAGAGAGCATCTTCTTAAATGGAGGG10.5813953488372093No Hit
ACTCTGTCTCAAAAAACCAAAAACAACAACAACAAACAAAAACAAAAAAC10.5813953488372093No Hit
GATGTAGGCCTCGTTCGTGAGGCCGTAGATGGCGGGGATCGAGTCGAGCG10.5813953488372093No Hit
GTCTGCGACTTGCCGCAGCCCGGCGGCCCGTCGAGCACGAAGCTGTGCCC10.5813953488372093No Hit
GAGTACATGGGTACGGACCCCGAGATCTACACTCTTTCCCTACACGACGC10.5813953488372093Illumina Single End PCR Primer 1 (100% over 31bp)
GTATATGGGATATGGGAACCCTCCTTTCCTTCCTCTCAGTTTTGCTGTGA10.5813953488372093No Hit
CCTAGGAAGTCTACAGTAGCTGGACACAGTGAACATCCTAGACCTGGAAG10.5813953488372093No Hit
TGGGAATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGTCG10.5813953488372093Illumina Single End PCR Primer 1 (97% over 46bp)
TATCAACGCAGAGTACATGGGAATGTAGGCGACACCGAGATCTACACTCT10.5813953488372093No Hit
CTCCCCCTCTCCATAATAAATGCCTTAGAAAGTTTAAACAAGCAGAATGT10.5813953488372093No Hit
GCATAGTACATGGGTGATACGGCGACCACCGAGATATACACTCTTTCCCT10.5813953488372093Illumina Single End PCR Primer 1 (97% over 36bp)
ATTCAGCTGCGTCTTCGGGAAGGGGCTCGTCGGTCACGCGGGAATGTCTT10.5813953488372093No Hit
TTCCTGTTCTGCCGCCCTTGCAGAAACATTCCTCAACTGCCACGCGGGGG10.5813953488372093No Hit
CTCTTCCGATCTCTGGTACCTGTCCCAAGTTCTGCAGGTACCAGAGATCG10.5813953488372093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGTTGATACCACTGCTT10.5813953488372093Clontech Universal Primer Mix Long (95% over 21bp)
CCCTGGTGGTGGGGGCGCCTCCCCCCCGGGGACCGGGCTGGGCTTGGTGG10.5813953488372093No Hit
GGGTTTGGAAGAATTGACTGGTGATTTCTTCTTTTTTAAAGTCCTTAATG10.5813953488372093No Hit
GAGTCTCGCTCTGTCGCCCAGTCTGGAGTGCAGTGGCACGATCTTCGCTC10.5813953488372093No Hit
CGTTTAATGCGGCCGTCTTTGACCGCGACGTCCAGCGCGAGCGGAACGGA10.5813953488372093No Hit
GATAGCTTGCTGAGCACTGATGACGCCCGCGCTACGATCGAGGGCTCCAA10.5813953488372093No Hit
ACCACGCTCAGTCCAGCTCCACTCACAGACAAGAGTCAAAGTCGATATTT10.5813953488372093No Hit
GGTGTACGGGCCACCGAGATATACACTCTTTCCCTACACGACGCTCTTCT10.5813953488372093Illumina Single End PCR Primer 1 (96% over 28bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAGAAAATAAAAAAAAAAAAA10.5813953488372093Clontech Universal Primer Mix Long (95% over 22bp)
ATATAGAACAACTAGATGGGAATAGCACAGTAGTCATTGGCCCTGGTGGT10.5813953488372093No Hit
GTTTTGAAACACTCTTTCTGTGGAATTTGCAAGTGGAGATTTCAAGCGAT10.5813953488372093No Hit
TGCCCGCCGAAACCGAAGCGCATCGCCGACAGCACCTTGTCGCCGAACGT10.5813953488372093No Hit
CTGCGGTCAGTGTGCCAGGGCCCGCAGGGTCGACCGTCTGCCCTGGGAGA10.5813953488372093No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATAGGCGCC10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAGAGTACATGGGTG10.5813953488372093Clontech SMARTer II A Oligonucleotide (95% over 23bp)
AAATAAATGTTCTCAATAGACCAGGTGTGGTGGCTCACGCCTGTAATCCC10.5813953488372093No Hit
GCACGCTGCTCCCTCCTGCAAGCTCTACTGCTTCATCTTGCTCTGTTCCT10.5813953488372093No Hit
GGGCACCCAACCCGGAACCGACCATGATCGCGGTCGGGGTGGCCAGACCC10.5813953488372093No Hit
GTACATGGGTGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACG10.5813953488372093Illumina Single End PCR Primer 1 (100% over 41bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTCAAGGGGGCCTACGACGAGCCGGAGGACGTGGCGTTCACCGATCGTCA10.5813953488372093No Hit
CTTGGGCCTGGCCTCCTCACGTGCCACTGGACAAGACTTACTCAGTCTCT10.5813953488372093No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATACAAGTAGTATAGCATGTTTCTCTAAGGCATTCAAGACTCGATAAAC10.5813953488372093No Hit
CAACCGCCGGATCGCGGTGAACAGCCCCAGCGTCCCACCCTCCGCCGCCA10.5813953488372093No Hit
AGACTCGGTGGCTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10.5813953488372093Clontech Universal Primer Mix Long (96% over 26bp)
CATCTACACCGTGGTGCGCAGCGGGCGCGTCGATTTGACCGAGTTCGCCG10.5813953488372093No Hit
CTCCTGAATCTATCTTTGACAAAATATACAGCACCAAGAGCGACGTGTGG10.5813953488372093No Hit
ACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGCTGTC10.5813953488372093Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGATCTCT10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTAAAGCGCGCGCAGGTGGTTTAATAAGTCTGATGTGAAAGCCCACGG10.5813953488372093No Hit
GTCTCTCGTCGCACAGGCTGGAGTGCAATGACGCGATCTCAGCTCACTGC10.5813953488372093No Hit
CCCGACGCCCGCGCCGACCGCGAGCCCGCAGCCTGGCTTCGTCCAGCCGT10.5813953488372093No Hit
TGGGAATGATACGGCGACCACCGAGATCTACACTCATTACCTACACGACG10.5813953488372093Illumina Single End PCR Primer 1 (97% over 34bp)
TCACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAGAGATC10.5813953488372093No Hit
GCCGTCTACATCGACCGCCGCGAACTCAAATGCGCATGCGTCGGCGGATC10.5813953488372093No Hit
CGCCTGCCCCGACCGCACCGACCTGCGCGTGCCCGGCCAACAACACCGCG10.5813953488372093No Hit
GCGGAAAGCGCGCGCAGGTGGTTTAACAAGTCTGATGTGAAAGCCCACGG10.5813953488372093No Hit
ACGTCACTCAGATGATGGCGGCATCTTGCGTCGAGACGAGTGTATCGTCG10.5813953488372093No Hit
AGGTTTCACGAAGCGCGCCATGATCGCGCAGTCCAACATCGATCCTGAGC10.5813953488372093No Hit
GAGCAAGACTGTCTCAAAAAAATAATAATAAATAATAAATAAAAAAACAG10.5813953488372093No Hit
GGCCCATCATGACCTAGGCTCTTTCTCTCTTTTCAGTTTGGTCTTCCCCA10.5813953488372093No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATCTACACTCT10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACCGACCCGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTAT10.5813953488372093Illumina Single End PCR Primer 1 (100% over 38bp)
GTACATGGGAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATACA10.5813953488372093Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCCCCAACCGCCGCGCGTTGGCCGCCGCGACCCTGAGCGCGTGCGGCGAC10.5813953488372093No Hit
ATTAAGAAGTTAGAGGAAAGATGACTTAGTGCCCAATTCAGTATGAAAAG10.5813953488372093No Hit
TCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTAC10.5813953488372093Illumina Single End PCR Primer 1 (100% over 25bp)
GGACATGGGAGTACGGCCACCGAGACTAACTTTCCCTACACGACGCTCAT10.5813953488372093No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATACGGCGA10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCGAACAGCTGCCCTTGTGGCTCCCCGTCGCGCTCGGCTTCGGGATCAC10.5813953488372093No Hit
GTACATGGGAGTACGGCCACCTAGACTAACTTTCCCTACACGACGCTCTT10.5813953488372093Illumina Single End PCR Primer 1 (95% over 22bp)
GTACTGCCTCGATGGTTTTGGACATGCTCCAGGGGAATTCTCTGGATTAT10.5813953488372093No Hit
GTCCCATCATGACCTAGGCTCTTTCTCTCTTTTCAGTTTGGTCTTCCCCA10.5813953488372093No Hit
GTCGAAGGTCGAGAAAAAGAAGCTTTTGCCGTTCCGGAATTTATGAAAAA10.5813953488372093No Hit
TTCCGGTTCGGCCGCCCTTGCCGAAACCTTCCTCAACTGCCTCGCGGGGG10.5813953488372093No Hit
GTTCCTGTCTGACTAGATGATCATGGTGCTGTTCCTGTTCCTGTCTGACT10.5813953488372093No Hit
GGAAACGGGAATATCGTCATATAAATTGTAGACAGAAGCATTATCAGGAA10.5813953488372093No Hit
GCCTCTAGCAATTCATGAATTATAGTTTAGGTTTTCCTATCCTGGTACTG10.5813953488372093No Hit
GTTCAACCCGTTCTTCCAGAGCCGCTACGGCTTCCCGAACACCAAGACCT10.5813953488372093No Hit
GGTTCACGCCATTCTCCTGCCACAGCCTCCCAAGTAGCTGGGACTACAGG10.5813953488372093No Hit
CAGTGGTAACAACTCAGAGTACATGGTAACCCTGTCTCTTATACACAGCA10.5813953488372093No Hit
CAGTTATTTCTTCTGATACAACTTGGGATGTGAAAAACATCCTAATGGAC10.5813953488372093No Hit
GATTATAAATCATTCTGCTATAAAGACACATGCAAAATTATGTTTTTTGT10.5813953488372093No Hit
GCCCTGAAGCTGATGGTGCAGGCCTGGCCTTTCCTCCGCCTTCCTCTGGG10.5813953488372093No Hit
GGTCTGGGGGAGGTCATCCTGGCCTTCAGATCGATTTATCCCTGTCTCTT10.5813953488372093No Hit
GGCGGCGGCCGCCAGCGCGTTGGCGATGTTGAACTCGGCCGTTCCCGCCA10.5813953488372093No Hit
AGGTAACTTTTTATTCTTAAGATTGTACTAAGAAATAATGAAGTGGAAAA10.5813953488372093No Hit
GTACAAGGGAAGCAGGGGTATCAACGCCGAGTACATGGGATCTACACTCT10.5813953488372093No Hit
GGGATATCGCGGTCCGGCCTTCGGGCAACCCGGGCTATTACCACACGAAT10.5813953488372093No Hit
CGCTCGGGAATCCAGGCCGTCTCGGTGCGCACTGCAGTCAGGTCGCGGAT10.5813953488372093No Hit
GTATCAACGTAAAAAAAAAAATAAAAAAAATAAAAAAAATTACCTTGCAA10.5813953488372093No Hit
CCCTACATGACGCTCTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTACC10.5813953488372093Illumina Single End PCR Primer 1 (95% over 24bp)
TGCGATTTCATTCCAATCGATTCAATTCCATTCGATGACATTCCTTTCGT10.5813953488372093No Hit
GGCGACCACCGAGATCTACATCTTTCCCTACACGACGCTCTTCCGATCTC10.5813953488372093Illumina Single End PCR Primer 1 (96% over 30bp)
GTGTACAAATCCTGCACATCTTTGTTATTTTATTTATTCTTTTTGATGCA10.5813953488372093No Hit
AGAGAGCACCGTGCGGCTATCTGCCGTCGTCACCCCAAAGGCAACCAAGG10.5813953488372093No Hit
GTTTCTTGGTGCAAACACAGCCCTGGGAAATGGGTCAGGTAAAGAGCAGG10.5813953488372093No Hit
GGACAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTCTGTCTCTTATAC10.5813953488372093Illumina Single End PCR Primer 1 (96% over 33bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTA10.5813953488372093Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GATTTTCACTTCGCGAAACGAATTGATATACACCTTCAAACCTGTCTCTT10.5813953488372093No Hit
GGCCGCGAGAGGCGGGAGATGGGCTCCAGCACCCCCTTGCTCTCCGTCAG10.5813953488372093No Hit
GCACCACAGGACTTAGAGTCTGTGGAGGGGGAAGAGGAGGCGCCCGAGCC10.5813953488372093No Hit
GATCACCGGCAATGCGAGCGTCGAGACGTCGGTGCAGGCCCTTCGGCTGG10.5813953488372093No Hit
GTACATGGGTGATACGGCGACCACCGAGATCTACACTCTACTTGGGACAG10.5813953488372093Illumina Single End PCR Primer 1 (96% over 30bp)
GTACATGGGTACGGCGACCCCCGAGATCTAAACTCTTTCCCAACACGACG10.5813953488372093Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)