FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG32.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
GTCTGACTTGTGGAATTGCAGTATCAGTGTTCAGAAAACTTACCAGACTT32.564102564102564No Hit
TTCCCTACACGACGCTCTTCCGATCTATCCCTGTTGGTACCAAAAAAGGG32.564102564102564Illumina Single End PCR Primer 1 (100% over 26bp)
GCTCCAGGCCCCTCGGTGGGTGTTCAAGGAGGAAGACCCTATTCACCTGA32.564102564102564No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.7094017094017095Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.7094017094017095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATAGAGACCATCCTGGCTAACACAGTGAAACCCTGTCTCTATTAAAATT21.7094017094017095No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT21.7094017094017095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAGATCCGAACCTTTTCTAAATTTGGGCTTTACTTTCGAGGGTTCCTCC21.7094017094017095No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.7094017094017095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTGGGTTGTTACCCCGGCTTCCCATGTA10.8547008547008548No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT10.8547008547008548No Hit
AATAAACAATAACAAAAAACGACACAATTCACATATAAACAACACCGAGC10.8547008547008548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8547008547008548No Hit
CGGTATCCACCCATTCGGGGACTTTCAGCTTCCCGGACTTTTTGAGGAAG10.8547008547008548No Hit
GAATATTACGGCAGCTAAGGAAATTGAAGTTGGTGGTGGTCGGAAAGCTA10.8547008547008548No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCGATCATCCCATGTACTC10.8547008547008548Illumina Single End PCR Primer 1 (96% over 33bp)
GATCACGTATATACCACTTCCATTTGATGATGGAATGCTGCTGTGCATGA10.8547008547008548No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAA10.8547008547008548No Hit
GATCGGAAGAGCGTCGTGTAGGGAAGGTTATCTCGGGGTCGCGTACTCCC10.8547008547008548Illumina Single End PCR Primer 1 (96% over 27bp)
AACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAG10.8547008547008548No Hit
GACCTTGTCTCTACTAAAAATGCAAAAATTATCTGGGCATGGTGGTGGGC10.8547008547008548No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCCAGAGTACATGGGA10.8547008547008548Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG10.8547008547008548Illumina Single End PCR Primer 1 (100% over 22bp)
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGTTATAACACATC10.8547008547008548No Hit
GCTTGTATCAGACCAAGAGCTGCAAGAGTTAAAAATGAGAACTCCCCACA10.8547008547008548No Hit
GATCGGAAGACGTCGTGTAGGGAAAGATAGATCTCGGGGCCACTGCCCAT10.8547008547008548No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTATTCGTTTATACCA10.8547008547008548No Hit
GTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCAT10.8547008547008548No Hit
GAGTACATGGGACGCCACCGAGCTAACACTTGCCTCAGATTCTGTCGGTA10.8547008547008548No Hit
AACGCAGAGTACATGGGACGGCGACACAGACAACTCTTTCCCTACACGAC10.8547008547008548No Hit
ATCTGAGGCAGCGTCGTGTAGGGAAAGTGAGATTCCCTCCCCCTGTCTCT10.8547008547008548No Hit
GTANCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACACG10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGT10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTATGCGTTGATACCAC10.8547008547008548No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCA10.8547008547008548Illumina Single End PCR Primer 1 (100% over 38bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACA10.8547008547008548Clontech SMART CDS Primer II A (96% over 25bp)
GGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTC10.8547008547008548No Hit
CCTTGATGTCGTCGCGTAGCCGCCCGCGCTCCTGTCTCTTATACACATCT10.8547008547008548No Hit
GCTAGTAGCGGCAGGAAGATGTCAGGCTCACTTTCCTCTGATTCCCGAAA10.8547008547008548No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATACC10.8547008547008548No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTAAGCGTTTAAAACA10.8547008547008548No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGTACGGGACCCCGAGTCTACCTCTTCCCTACACGGAGCTGCCGAT10.8547008547008548No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTGGGTTGATACCACTGCTTCCTGTC10.8547008547008548No Hit
GCTCTGGTCCGCTTGGCCTTTCTCTCCAGCTGCAGTAGCATGATGTCGTT10.8547008547008548No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGC10.8547008547008548No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.8547008547008548Clontech SMART CDS Primer II A (100% over 26bp)
GTGCGGTCGCGGGCGCTGTCGGGTGGGCTCAGGAGGCTGTAGGAGGACTG10.8547008547008548No Hit
GCCGTGGTGGTGGCAACCCGGTCCGACTGCACTGCGGTCCGCGTGCTGAT10.8547008547008548No Hit
GCAAAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTCTGCGTCTGT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT10.8547008547008548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8547008547008548No Hit
GTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTA10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTATTTAAATAGCAATAGATAATCAAGACTTAGTGGCTCACACCACTAAT10.8547008547008548No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACACA10.8547008547008548No Hit
GCATCATATTCGGAAATATCTTCCTAGGTTTATCTACCATTTAGTGTTGT10.8547008547008548No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.8547008547008548Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCA10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
ATCACAGTTGTCTCATTCACCCTCAGTGATCTCCGTCCAAGAGGCAGCAC10.8547008547008548No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
AGAAAGTGATGGCTCAGGGCAGCATTGGGGTGGCACCTGGTATGAACAGA10.8547008547008548No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATTACGGCG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GAGAAATATTTTTCGAGTGTGGGGAGGACAAGGAAAGAGTAGTGCAGGGG10.8547008547008548No Hit
GTACATGGGTGAAGCCACCGAGATCTACATCTTTCCCTACACGACGCTCT10.8547008547008548Illumina Single End PCR Primer 1 (95% over 22bp)
GGTATACATCATACATTTATTAGTGAATATCCCTCTGAAATCAGCAACAA10.8547008547008548No Hit
AATACATAGTGTAACTTGACACTAACAATTTCCAGACTCAAGGAACATGT10.8547008547008548No Hit
GTTCCAAAGCCAGTGAAATGTGAAGGAAAGTGGGGTCCTTCGGGGCAATG10.8547008547008548No Hit
GAGTTTGGTGGACTGGGTTGAAGTTTACCTTTATTTAGCAAACTCTTTCT10.8547008547008548No Hit
TCACAGAGTTGAACCTTTCTTTAGAGAGAGCAGAGTTGAAACACACTTTT10.8547008547008548No Hit
GTGTAGGACGGGGAGGTCACGATGGCGCGACGTCAGCAGAAAATACATGA10.8547008547008548No Hit
GTACATGGGAACGACTTTTCCCTACACGGAGCTGCCGATACCAGTAAACA10.8547008547008548No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
TCTCGGTGGTCGCCGTATCATCCCATGTACTCTGCGTTGCTGTCTCTTAT10.8547008547008548Illumina Single End PCR Primer 1 (95% over 21bp)
GAGCAGCCTTTAGTCTGAGAGGGCTAACCCAAAGGCCAAGCATTTGATGC10.8547008547008548No Hit
CCAGCAGATAGTGTGGATGACCACGCCATTTTGGTGTACGATGCATGCCA10.8547008547008548No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACAAGTCGTTTTAACAACA10.8547008547008548No Hit
GAAATAAGACAGGCCAACAAAAACAGAAAACAGAGTGAAAAAAATGAACA10.8547008547008548No Hit
GTGTAGGACGGGGAGGTCACGATGGCGCGACGTCTGCAGAAATTTCATGA10.8547008547008548No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAACTGAGTTTATACCA10.8547008547008548No Hit
CACTAAACTACACAAATACGAACACAGTTAACTGAAATACTTTAGAAATC10.8547008547008548No Hit
CTCCGATGAACATTTACTTTGAGAATCATAGTGGTGCCCAATAAGTTTCG10.8547008547008548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTAATCGTTTATACCACATCAT10.8547008547008548No Hit
CCACATATACAAATGAGGAATTTTATTTTTTTATTTTTTAAAATTTTATT10.8547008547008548No Hit
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAGA10.8547008547008548No Hit
TATCAAGACATGTATAATTTTTTGTTTTGATCAGTATTTGTTACCTTTAA10.8547008547008548No Hit
GTGTAGATCTCGGTGTCGCCGTATCCCATGTACTCTGCGTTGATACCTGT10.8547008547008548No Hit
TAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGG10.8547008547008548No Hit
GGAGCCCCGAAACCGCCTAGGAACGCTCAGCCCCTGGGCTCGTGCAGGGC10.8547008547008548No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.8547008547008548Clontech Universal Primer Mix Long (95% over 21bp)
GATCTACACTCTTTCCCTACACGACGCTCTTGCCCCAGATTCTGTCGGTA10.8547008547008548Illumina Single End PCR Primer 1 (96% over 33bp)
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAGAGTACTGGGACG10.8547008547008548Clontech SMART CDS Primer II A (100% over 24bp)
GCAGTGGTATCAACGCAAAAAAAAAAAAAAGTACCTGTCTCTTATACACA10.8547008547008548No Hit
AACTAAGAACGGCCAAGCACCACCACCCACGGAATCGAGAAAGAGCTATC10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATCTGCGTTGAAAACA10.8547008547008548No Hit
ATAGTGTAGATCTCGTGCGCCGTATCATCCCATGTACTCCTGTCTCTTAT10.8547008547008548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)