FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54.273504273504273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG43.418803418803419Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG43.418803418803419Clontech Universal Primer Mix Long (96% over 26bp)
GGCTTAAATCACTTTCAAGAGAAGAACCCACAGATGAAATTGCACTTGTG32.564102564102564No Hit
GGAGTACCATCACCAAGCAGAAAGAGACTTGGTACCCAGGTGGAAAGAAT32.564102564102564No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGCAAAAAAAAAAAAAAAAAAAAA21.7094017094017095Clontech Universal Primer Mix Long (96% over 25bp)
GCTCAGAGGGGACCTGTAGAAGGTATACACAACCTGAGGTCCTCACAGAT21.7094017094017095No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.7094017094017095Clontech Universal Primer Mix Long (96% over 26bp)
CTTGAGGGCTAATATTCCACTATTTGTGTAGATAGATGGATATCTCACAA21.7094017094017095No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT21.7094017094017095Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA21.7094017094017095Clontech Universal Primer Mix Long (95% over 22bp)
TCACAGGGCAGGGGACCCTGGAGCATCAGAGGGCGCTCATCCAGCTGGCG21.7094017094017095No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA21.7094017094017095Clontech Universal Primer Mix Long (95% over 21bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTTTTCCCAAGCCCCTCAGCCAAAGCAGGGAGCAGGGTAGAGCTCCT10.8547008547008548No Hit
TCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAA10.8547008547008548No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACA10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
CCATTTGGCGGAACCTAGTGTTTTTCTACTATAGGTTCAGCCATCTCTCT10.8547008547008548No Hit
GTACATGGACGCGTCACGAGATCAACACACATTCCCAAAACGCGCGCTTC10.8547008547008548No Hit
GTATCTTTAGTGATGCGGAGAAACTACTGGAATGCGACCCCAGGGATACA10.8547008547008548No Hit
GAGGACAGGTACTGACTGGCAACATTCATGGGCAGAAAAAAATCCAGGTG10.8547008547008548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8547008547008548No Hit
GAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT10.8547008547008548Clontech SMART CDS Primer II A (100% over 26bp)
AACGCAGAGTACATGGGATGATCGCGACCACCGAGATCTACACTCTTTCC10.8547008547008548Illumina Single End PCR Primer 1 (100% over 27bp)
CNCGTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAATTA10.8547008547008548Clontech Universal Primer Mix Long (95% over 21bp)
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA10.8547008547008548No Hit
GTACTTTTTTTTTTTTTTGCGTTGATACCACTGCCTGTCTCTTATACACA10.8547008547008548No Hit
GGCCTAAGCAAGGTGTCTGCACAATTTTATCTTGTACAGTACAGATGTGT10.8547008547008548No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGATACGG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGTCAGGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
ACACAGCTCTTAAGTGGCAGAGCCTGGCTTCAAATCCCATCAGCTTCATC10.8547008547008548No Hit
GAGTACATGGGATGATACGGCGCACGAGATCTACACTATCTGTCTCTTAT10.8547008547008548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTAAAAAG10.8547008547008548No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACAGCTTCC10.8547008547008548No Hit
TGAAGGTATGTTTTCCAAGTTGGTTCCATTCTTGTCTCTTTCAGGTACCC10.8547008547008548No Hit
GGGGAGGGAATCTCACTTTCCCTACACGACGCTGCCTCAGATCTGTCTCT10.8547008547008548No Hit
GATCTCGGTGGTCGCCGATCACCCATGTACTCTGCGTTGATACCACTGCT10.8547008547008548Clontech SMART CDS Primer II A (96% over 25bp)
CAAATCTGCTCTCTCTAAAGGAAGGTTCAAATCTGTGAGTTGAATACAAA10.8547008547008548No Hit
GAGGGGGGGGCGGCCCGCCGGCGGGGACAGGCGGGGGACCGGCTATCCGA10.8547008547008548No Hit
CCTCAAGGATGGCATCGTGCACAGCTGTCAGAGTACGGCTCCTGGGACGC10.8547008547008548No Hit
ATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGG10.8547008547008548No Hit
GAGCGCGGGCGGCTACGCGACGACATCAAGGCTGTCTCTTATACACATCT10.8547008547008548No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAATTAATCTGCGTTTATACCACTG10.8547008547008548No Hit
ATTAATTGGCGTAAATGCAACCATATAAAAACATAAGTTATGAAAAACAC10.8547008547008548No Hit
CAAATAACCTGTGTTCTCCTTTCCCTTTTCCTCATTTTATTTTCTTCTGT10.8547008547008548No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTAACAA10.8547008547008548No Hit
CTCCTCATACTGCCGCTGGTCAGCTTCATTGATCACTCCCTGAGCAAAAG10.8547008547008548No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCT10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGATACGGCGACACCGAGATCTACACCTGT10.8547008547008548No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACACTGCGTTGATACCAC10.8547008547008548No Hit
GTTTGGTGGGGATAAAATAGAGAACACTGAAATGGTAAAACCACAATAAT10.8547008547008548No Hit
ACAGTTATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCC10.8547008547008548No Hit
GTACATGGGTGATACGCCCCGAGATCTACACTCTTTCCCTACACTCTGTC10.8547008547008548Illumina Single End PCR Primer 1 (96% over 28bp)
GTCTCATTCTGTTGCCCAGGCTGGAGTGCAGTGGTGTGATCTCGGCTCAC10.8547008547008548No Hit
CTGCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCGAGATTAGTGGTGTGAGCCACTAAGTCTTGATTATCTATTGCTAT10.8547008547008548No Hit
CCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGC10.8547008547008548No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA10.8547008547008548No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGAC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAATGATACGGCGACCACCGAGATCTACACTTTTTTTTTT10.8547008547008548Illumina Single End PCR Primer 1 (97% over 34bp)
CTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGGA10.8547008547008548Illumina Single End PCR Primer 1 (100% over 25bp)
GGCCTGTTACTGGGCTTTGGCGGAAACGGAACGTTTGACTATGTGTCATC10.8547008547008548No Hit
GCTTGCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAGTACTCCTGTC10.8547008547008548Clontech SMARTer II A Oligonucleotide (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
CTACTAAAAGTACAAAATTAGTCGGGTGTGGTGGCGCATGCCTGTTATCC10.8547008547008548No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGTATC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GCTAAGTACAGAGCCATGTGGCCAGTGCAATGTCTATTTAAGGAAAAGGG10.8547008547008548No Hit
GCAGTGGTATCAACGCAGAGTACATGGGCCCCGAGCTCACTCTTCCCAAC10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATATATATAATACAACATACTAGTTATGTTAAATGCTACAAACCAATGTG10.8547008547008548No Hit
ACATGGGCAGTGGCCCCGAGATCTATCTTTCCCTACACGACGTCTTCCGA10.8547008547008548No Hit
GGTCCTGAAAAACGAGAGCGTCGTGTAGGGAAAGAGTTGTCTGTGTCGCC10.8547008547008548Illumina Single End PCR Primer 1 (95% over 24bp)
GGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAG10.8547008547008548Clontech Universal Primer Mix Long (95% over 24bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCT10.8547008547008548No Hit
ATTTATACTAGGGATGAAGAAAGAGTTTGCTAAATAAAGGTAAACTTCAA10.8547008547008548No Hit
GCTGCTCACTGTTGGGGAAGCTCCATAAATGTCACCTTGGGGGCCCACAA10.8547008547008548No Hit
GGCTGAAGCCGCAGACACCGGTCGGGCCTCTGCGGAGAACGCCAGTAGCA10.8547008547008548No Hit
CAACGCAGAGTACATGGGATGATACGGCGACCACCGAGACTGTCTCTTAT10.8547008547008548Illumina Single End PCR Primer 1 (95% over 22bp)
ACGCAGAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTTTGCCTGT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GGCCCGGGGGGGTGGTGACACAGCTCCCAGCGCGGCCCCCACCTGCCCTG10.8547008547008548No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATC10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGAC10.8547008547008548No Hit
GTTGTTTATCAGCCAGGGCCATTGGACAAGTTCTGTGACTTCCTTATTCC10.8547008547008548No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC10.8547008547008548No Hit
TATCAACGCAGAGTACATGGGTGATCGGCGACCACCGAGATCTACACTCT10.8547008547008548Illumina Single End PCR Primer 1 (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.8547008547008548Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAATTTTGTT10.8547008547008548No Hit
ACCATGTACTCTGCGTTGATACCATTGCTTCCCATGTACTCTGCGTTGAT10.8547008547008548Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACA10.8547008547008548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)