FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC43.7735849056603774Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG43.7735849056603774Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32.8301886792452833Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGCCGCCGTCGTCGATCGAGGACGCGATGTCGCATCAGATGCGTCACTG32.8301886792452833No Hit
TCCCGACGTTGTCGATCACGGTACGAAACAGCGGCCACTCGTCGTAGGCG32.8301886792452833No Hit
GTTTAATAGGAATAGCATTGAATTTGTGAATTGCATTGGGCAGTACGGCC21.8867924528301887No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT21.8867924528301887Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTAGTATCAACGCAGAGTACATGGGAA21.8867924528301887Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.8867924528301887Clontech Universal Primer Mix Long (96% over 26bp)
ACGCTCTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGCGTCGTGTA21.8867924528301887No Hit
CCTGTATATTTCACATGTTGTGTTCCTTAATGCCCATGGTTAAGACATTG21.8867924528301887No Hit
GTGTAGGGAAAGAGTGTAGTTCGGTGGTCGTTACCCATGTACCTGTCTCT21.8867924528301887Illumina Single End PCR Primer 1 (95% over 22bp)
GTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGACACCCATGTAC21.8867924528301887Illumina Single End PCR Primer 1 (97% over 38bp)
ACGCGCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGAGCGTCGTG21.8867924528301887No Hit
TCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGTCCA21.8867924528301887Illumina Single End PCR Primer 1 (100% over 36bp)
GTATCAACGCAGAGTACATGGTAATGATACGGCGACCACCTGTCTCTTAT21.8867924528301887No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTT21.8867924528301887Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCCGAGAACAAGTGGGAGCATGCGCTGGTATTCGTCAAGACGCGCAACGG10.9433962264150944No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCCTGT10.9433962264150944No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGGAGCCCATCGCGTATG10.9433962264150944No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCCAGGAGTCTGGTATGAATGAATTAAATACACACACTTTGATGACTC10.9433962264150944No Hit
GGTTCAGACATTTGGTGTATGTGCTTGGCTGAGGAACCAATGGGGCGAAG10.9433962264150944No Hit
ACAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAGGG10.9433962264150944Illumina Single End PCR Primer 1 (100% over 24bp)
GTGGTCGCCGTATCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
ACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAG10.9433962264150944No Hit
GGTTCAAGCTGCTGACCTTTCGCGAGACCTGGGCCTATGCCGCCGGCAAG10.9433962264150944No Hit
GCGTCGTGTAGGGAAAGGTGTAGATCGGGTGCTTCCCATGTACTCTGCGT10.9433962264150944No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGGACACCTG10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCACGTCATTGCACTTCAGCCTGGGTGACAGAGCGAGACCTGTCTCTT10.9433962264150944No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTG10.9433962264150944Illumina Single End PCR Primer 1 (100% over 38bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGACAAGATCC10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTT10.9433962264150944Illumina Single End PCR Primer 1 (100% over 38bp)
GTACTGGGTGATACGGCGACCACCGAGATCTACACGCTTTGCCTTAGACG10.9433962264150944Illumina Single End PCR Primer 1 (96% over 32bp)
CCACTACACTTCCCTACCGGAGCTGCCGATACCAGTAAACATGAGATCGG10.9433962264150944No Hit
GAGTACATGGGACCAGTCCTCTTTCCTACACGACGCTCTGTCCCAAGTTC10.9433962264150944No Hit
TATCAACGCAGAGTACATGGGAAGCATGGTATCAACGCAGAGTACATGGG10.9433962264150944No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9433962264150944Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACATGGGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.9433962264150944No Hit
GTTCTGTAAATGGGATTAATTATTCTCAAGTAATGTGGCCAATTCCTCTG10.9433962264150944No Hit
TATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAATTCT10.9433962264150944No Hit
CAACCTGGCTGCAACCGCCCATCAGTGCCGATACGGCGAAACCACCGGTA10.9433962264150944No Hit
GAGTGAGATCTCGGTGTCGCATCCCATGTACTCTGCGTTGATACCACTGC10.9433962264150944Clontech SMART CDS Primer II A (95% over 24bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA10.9433962264150944Clontech Universal Primer Mix Long (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGTATCCGACACGAGATCTACACTTTTCCCTAC10.9433962264150944No Hit
GACCGAGGCGTTCCACGACACCGTCAACACGGTCACCGATCTGCGTGACA10.9433962264150944No Hit
TCCATGTACTCTGCGTTGATACCCTGCTTCCCTGTACTCTGCGTTGATAC10.9433962264150944No Hit
TCGTGTAGGGAAAGAGTGTAGATCTCGGTGGCGCCGTCACCCATGTACCT10.9433962264150944Illumina Single End PCR Primer 1 (96% over 31bp)
ATCTGCAAGTGGATATTTGGATAGATTTGAGGATTTCGTTGGAAACGGGA10.9433962264150944No Hit
CTCTTTCCCTACACGACGCTCTTCCTGTCCCAAGTTCTGCAGGTACCAGA10.9433962264150944Illumina Single End PCR Primer 1 (96% over 25bp)
GGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACACCCATGTACTCTGCGT10.9433962264150944Illumina Single End PCR Primer 1 (96% over 32bp)
GGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGT10.9433962264150944No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTACACTCTTTCCCTACACGACGCTCCCCGGGTCTTGTCGATACCAGTA10.9433962264150944Illumina Single End PCR Primer 1 (96% over 27bp)
TTCCCTACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCT10.9433962264150944Illumina Single End PCR Primer 1 (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGCCTGTCTCTT10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACATGCAAAGAATTGATATCAACGCAAATTACACTTAAACAAATCTAA10.9433962264150944No Hit
GTCACTCTAAAGAAATAACTTATGTAAAACTCTTAGTAACCCTGTTTGTC10.9433962264150944No Hit
GTCAATGAACTGTGCATATCGACCGCGGACTCCGCCGGTTTGTCGGTGGA10.9433962264150944No Hit
GTACATGGGAAGCAGTGGTATTAACGTAAAAAAAAAAAAGGGTACTTTTT10.9433962264150944No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGTGATACGGCGACACCGAGATTACACTCTTTCCCT10.9433962264150944No Hit
ATCAATCAGCACCACTGGGAAATGGAAAAAGAGTTCCAGAAGTCCTCGTT10.9433962264150944No Hit
AATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATTGGACTGTCTCTTA10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAGTAC10.9433962264150944No Hit
CAACATGCAAGCACTGGAATAAAAGCAAATTACAATTATACACAACTCAT10.9433962264150944No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGATGTACGGCGAC10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCTACACGACGCGCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAA10.9433962264150944No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATTTC10.9433962264150944Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAA10.9433962264150944Clontech Universal Primer Mix Long (95% over 24bp)
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
CACGCATTTTTATCTGGTCGCCGCTGGTGCGCGGCTATCTTTTCTGGCTG10.9433962264150944No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAGGGGTTTTTAA10.9433962264150944No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.9433962264150944Clontech Universal Primer Mix Long (95% over 21bp)
GCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCA10.9433962264150944No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGGGTGCCGACCCATGTACTCTGCGT10.9433962264150944Illumina Single End PCR Primer 1 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTAAAAAAAATAAAAAATAAAAAAAT10.9433962264150944No Hit
GTATGGTGGTATAAGCCTGTGGTTCCAGCTTCTCAGGAGGCTGAGGTGAG10.9433962264150944No Hit

[FAIL]Adapter Content

Adapter graph