FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32.8301886792452833Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGACCAGGCTCACTCGAGGATCGGATCGCAACAGGTCCGCATAGGAGCCG32.8301886792452833No Hit
TACCCGGCCCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC32.8301886792452833Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGTCACTCTTTCCCTACAAGACCTGGGACAGATCGGAAGA32.8301886792452833No Hit
CTCGAAGACCTCCGCGCCATCCCGTGGGTGTTCGCGTGGTCGCAGGCGCG32.8301886792452833No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA32.8301886792452833Clontech Universal Primer Mix Long (95% over 21bp)
GGCAACTGTACTTCTTTAACAAATGTGTCTTCTTCTAAGTGGGCAGTGTC21.8867924528301887No Hit
GTACATGGGTAACGACCACCGAACTACACTCTTTCCCTACACCTGTCTCT21.8867924528301887Illumina Single End PCR Primer 1 (95% over 22bp)
GTATCAACGCAGAGTACATGGGACGGGACAGATCTACCTCTTTCCCTACA21.8867924528301887No Hit
GGAATACAGCTAACCAGGGAGGTGAAAGATCTCTACAAGGGGAACTACAA21.8867924528301887No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGTATCAACGCAGAGTACAGGGA21.8867924528301887No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.8867924528301887Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACTACATGGGGTCGGGACACCAGACTCACCTTGCAGTCTGCGTTGATAC21.8867924528301887No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.8867924528301887Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGATCGGCACCCCGAGATCTACACTCTTTCCC21.8867924528301887Illumina Single End PCR Primer 1 (95% over 23bp)
GTGGTCGCCGTATCATTACCATGTACTCTGCGTTGATACCTGTCTCTTAT21.8867924528301887No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTT21.8867924528301887Clontech SMART CDS Primer II A (96% over 25bp)
GTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACAT10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGCTGT10.9433962264150944No Hit
GTATCAACGCAGAGTACATGGGTGATACGGCGACCACCGAGATTACACTC10.9433962264150944Illumina Single End PCR Primer 1 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
GGAGTGCATCCAGTTTGTCCAGGCTGGCGGTGATGGCATTGAATTTGTCA10.9433962264150944No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGTCT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
GGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCG10.9433962264150944No Hit
TATCTGTGGTGGTGGGCAAGAATGGGAAGGGTACAGAATGGGCAGCCTGA10.9433962264150944No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGTACGGCGACCACCGA10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA10.9433962264150944Illumina Single End PCR Primer 1 (100% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.9433962264150944Clontech SMART CDS Primer II A (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAACCACTACAC10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAGAGATC10.9433962264150944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10.9433962264150944No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
CGATGGGCACGACCGCCAGGGCGCAGAGCAGCATGGTGGTCTTGCGCGCC10.9433962264150944No Hit
GATACGGCGACACCGAGATTTACATTTTCCCTACACGACGCTCTTCCGAT10.9433962264150944Illumina Single End PCR Primer 1 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC10.9433962264150944No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGA10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
NCGCAGAGTACATGGGAAGCACCCGATCTACACCTTTCCCTACACGACGC10.9433962264150944No Hit
ATCAGGCTGAAAAAGCCGGGCATACCGGCGCGCTGGATCCGCTTGCTTCT10.9433962264150944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.9433962264150944No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTAATCGTTTAAAACAAATAAAAC10.9433962264150944No Hit
TCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG10.9433962264150944Illumina Single End PCR Primer 1 (100% over 22bp)
GTATCAACGCAGAGTACATGGGTGATACGGCGACCACCGAGATCTACACT10.9433962264150944Illumina Single End PCR Primer 1 (100% over 28bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGGAAAGAGTGTAATCTCGGTGTCGCCGTATCACCCATGTACTCT10.9433962264150944No Hit
AACTCGGGTCGCGTATCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACCAGTCCTCT10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAGTCTACTTCCCTACCGACGCTCTTCCGATCTCTGGTACCT10.9433962264150944No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGTTTTAACACAGGATAC10.9433962264150944No Hit
GTACATGGGTGTACGGCGACCACCGCTCTTTCCCTGCTGGTACCAGTAGA10.9433962264150944No Hit
CATGGGAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10.9433962264150944Clontech SMART CDS Primer II A (96% over 26bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAGTGGTTTTAAACAAAG10.9433962264150944No Hit
TCATCCGCACACACCAGGGACACCGCCACGCCACTGGCGCCCGCGCGGCC10.9433962264150944No Hit
GCAATGGCATCGTTCTGGTGCCGGACATTTACGGTGCCAACCGGCACCCG10.9433962264150944No Hit
TCCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTA10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
CAGTCACAAAGAAGTTTCTGAGAATGCTGCTGTCTACTTTTTATATATAA10.9433962264150944No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGTCGGCACCCCGAGATCTACACTCTTTCCCTAC10.9433962264150944Illumina Single End PCR Primer 1 (96% over 26bp)
GTAAATGGGTGATACGGCGACCAACGATATCTACACTCTTACCCTACAAT10.9433962264150944No Hit
CGTTTACACTGCGTGTTTCGTTCACCACGCTGCCACTCAAACAGTGGACG10.9433962264150944No Hit
ACGCAGAGTACATGGGTACGCACCACGAGATCTACACTCTTTCCCTACAC10.9433962264150944Illumina Single End PCR Primer 1 (96% over 26bp)
GTCTCGCTCTGTCACCCAGGCTGAAGTGCAATGACGTGATCCTGTCTCTT10.9433962264150944No Hit
GGTGTGATCAGAGCTCACTGCAGCCTCACCTTCCAGGCTCAAGCAATCCT10.9433962264150944No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.9433962264150944Clontech Universal Primer Mix Long (96% over 25bp)
GGTACCCATAGGGCAGCACAAAGAGAGGCAGGTGACAAGTGGATGTGCAG10.9433962264150944No Hit
CACGGAATGAAACTGCTGGTGGATATTCAGCACGGACACAAAACGGGCTA10.9433962264150944No Hit
GTGTCCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTG10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
CACTTAAAAGCCCCCAATAAATAAAGCAAAAAGAAAGGTAAAACTGAAGT10.9433962264150944No Hit
GCTGTAGTTAGTTTTGATTCACTATATACTCTCTGTACTTGAGGAAGAGT10.9433962264150944No Hit
TATCAACGCAGAGTACATGGTGGAAGATACCTTCCTACACGACGCTCTTC10.9433962264150944No Hit
TCAACGCAGAGTACTTTAATTTTTTTTTTTTATATTTAATTATTTTCGCA10.9433962264150944No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGAGGCGTTTATAC10.9433962264150944No Hit
GTACATGGGTGACGGCGCCACCGAGATCTACACTCTTTCCCTACACGACT10.9433962264150944Illumina Single End PCR Primer 1 (96% over 33bp)
GAGTACATGGGTGTACGGCGACCACCGATCTACACTCTTTCCCTACACGA10.9433962264150944Illumina Single End PCR Primer 1 (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACAAAGCGTTTAAACCAAA10.9433962264150944No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTACATGTATC10.9433962264150944Clontech SMARTer II A Oligonucleotide (95% over 21bp)
ACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAG10.9433962264150944No Hit
GTACATGGGAATATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC10.9433962264150944Illumina Single End PCR Primer 1 (97% over 39bp)
ACACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAAC10.9433962264150944No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGG10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTGGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCT10.9433962264150944No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)