FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT43.418803418803419Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCACGTGGGGAGCGGTGGCTGAGAAAATGTAAGGATTCTGGAATACATA32.564102564102564No Hit
GAATAGTATTTTGAGGAAAGATGAATATATGGGATCTGGTCTGAATTGGG32.564102564102564No Hit
TGCCCAGCGAGGCCATGGGCATCGACTTCGAGGCCGAGATCGCCGTCATC21.7094017094017095No Hit
ATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGA21.7094017094017095Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.7094017094017095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATCT21.7094017094017095Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTTTGATCCACAGATGTCTCATATGGTGGAATGAGAGAACCAAACACAAG21.7094017094017095No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCGGGGGGGG21.7094017094017095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAACACCCGTCATCAACAGGGCACCACCTACCATGCCGCCACAGCACCTG21.7094017094017095No Hit
GCCCACAACGCCATTGAGAAGCGCTACCGCTCCTCCATCAATGACAAAAT21.7094017094017095No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT21.7094017094017095Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGCCGCTTCCGCCGGCGCCTCGCCGGCTTCGATCGCGCCCTTGG10.8547008547008548No Hit
CAGTAAACTGAAGAACTTGTAACAACTCACACTAAAACACAAATACCACC10.8547008547008548No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA10.8547008547008548No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGGCCTGTT10.8547008547008548Illumina Single End PCR Primer 1 (100% over 30bp)
GATCGGAAGAGCGTCGGTAGGATCTCTGCCCCATGTACTCTGCGTTGATA10.8547008547008548No Hit
ACTTGGGACAGAGCGTCGTGTAGGGAAAGATGTAGTCTCGTGGTCGCCGT10.8547008547008548Illumina Single End PCR Primer 1 (95% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTCAGATTGGCCTGGCAGCCCCTGGCACAGAGCAGACCCGGCCACAGG10.8547008547008548No Hit
ACGCAGAGTACATGGGATAGGGACACCGAGATCTACACTCTTTCCCTACA10.8547008547008548Illumina Single End PCR Primer 1 (100% over 27bp)
CAGACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATACATGGGAA10.8547008547008548Clontech Universal Primer Mix Long (95% over 21bp)
TCCACATCGGCCCGCGCGACCGGCTGGCGCTGATCGGGCGCAACGGCGCG10.8547008547008548No Hit
GTGGTATCAACGCAGAGTACATGGGATAACGGCGACCACGAGATCTACAC10.8547008547008548No Hit
ATCTATACGTTAACTTTTTTAACTGAGAAGCACCCAGGGAAGGGCCTGGG10.8547008547008548No Hit
ATATAACCTTGGGATATCCAATGGTTCATTAGATATGACCCAAAATCACA10.8547008547008548No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCATTATAAAA10.8547008547008548No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAGAGGG10.8547008547008548No Hit
GACCAGGCACAGTGGTTCACGCCTGAAATCCCAGCACTTTGGGAGGCTAA10.8547008547008548No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.8547008547008548Clontech Universal Primer Mix Long (95% over 22bp)
GCTTCCCCCCTGCATTGGAGATGCAGGGGGGAAGCAGCCCATAAATGGTC10.8547008547008548No Hit
ACACGAAGGATCCCTCCCCATTCAGTCCCATCTCTGACTCCGCATCTAGA10.8547008547008548No Hit
GTGTAGGGAAGGTTATCTCGTGTCCGATCCCATGTACTCTGCGTTGATAC10.8547008547008548No Hit
TGAAGGGGCTTATCCTGTTTGGAGCTTGTTCAGCTTCTTGAATCCTGGTT10.8547008547008548No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGCGGCACTTCCAACACGACGCGTGAAAAACGAGCG10.8547008547008548No Hit
TCCCTACACGACGCTCTTCCGATCTCAGTCCCCGCATCATGGTCTGAGAT10.8547008547008548Illumina Single End PCR Primer 1 (100% over 25bp)
CATTAAAAAAGAACAGTAGTATCAACGAATATAACATATACACAACACAT10.8547008547008548No Hit
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG10.8547008547008548Illumina Single End PCR Primer 1 (100% over 25bp)
CAATAAACAAAGACTACATTTGAAGCAAACTTAAAAAAAAACACAACACC10.8547008547008548No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGGGCAAAACCACACGAAACAACG10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGCAGCACATTCGAGACGGCGATGCAGTGCCCCGAGCTGTGGGGCATCA10.8547008547008548No Hit
CTGCCACATCTGTGCTTAGATTCCCTTAAGCTTTTGGATTTTAGGAAAAT10.8547008547008548No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGTAGATCTCGGTGGTCGCCGT10.8547008547008548Illumina Single End PCR Primer 1 (96% over 29bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGTACACCTCCT10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGGGTTGATACCACTGCTACGCAGAGTACATGGGAAGCAGTGGTAT10.8547008547008548Clontech Universal Primer Mix Long (95% over 22bp)
CAACATGCAAGAACTACAAAAGATATCAACATTAACATAAACAAATCTCA10.8547008547008548No Hit
ACATGGAATGATCGCGACCACCGAGATCTACCTCTTTCCCTACACGACGC10.8547008547008548No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTATGGGTTTTTAACAAAGCAACC10.8547008547008548No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGACCCCG10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCCAGAGTAC10.8547008547008548Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGCGTGTGTAGGGAAA10.8547008547008548No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTG10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.8547008547008548Clontech SMART CDS Primer II A (100% over 26bp)
CTATGATAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGC10.8547008547008548No Hit
CAAATGTTCAGTAAACACAAGATGCTCAGCATCATTAGTCATTAAGAAAA10.8547008547008548No Hit
CATCTACAATCATAACATACAACACCATAATCATATAAAAATAACCGAGC10.8547008547008548No Hit
CTCTATGAACTGATATCATGATTTTCTGTATATATTAAGCTGTCTCTTAT10.8547008547008548No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAACCACAGTGACGAAACCGATCGTTCCTAGCGTCTCCGACATGCCTGCA10.8547008547008548No Hit
CCATGGGATCGAGGCCGAGAACCGCCGCCTCGAAAGCGCCGCCGGCGATG10.8547008547008548No Hit
ATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.8547008547008548Clontech Universal Primer Mix Long (96% over 27bp)
TTCAAACCGGGGGAGAAGGTCACGGCAGAGCAGGCACACCAGCTCGGTGT10.8547008547008548No Hit
AGATCCATCTTTCCCTACACGACGCTCTTCCGATCTGGGCCTCCAGTTCC10.8547008547008548Illumina Single End Sequencing Primer (96% over 30bp)
GGGAAAGAGTAGATCTCGGTGGTCGCCGTATCTTACCCTGTCTCTTATAC10.8547008547008548Illumina Single End PCR Primer 1 (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTACC10.8547008547008548Clontech Universal Primer Mix Long (95% over 21bp)
TACTGTATGAGCGGGAAATCGCTGGACGCTTTGGAAGGATTCGCCTCGGC10.8547008547008548No Hit
GTCGTGTAGGAAAGTGTAATCTCGGTGGTCCGACACCCATGTACTCCTGT10.8547008547008548No Hit
ATCTCGGCCTCGCCCGGCTCTAACAGGCACGCGTACGCGCCGCCCTGGTA10.8547008547008548No Hit
CTTCAATTAACATCTGGTGAATAATGCCCAATGCACGGCGTATACAGTCA10.8547008547008548No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGTGAAGCAG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTCTAATATAAGCAGTCATACAAGGGATAAGTCCTCCAACCTGGATGCCG10.8547008547008548No Hit
GGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACTTCCCATGTACTCTGCG10.8547008547008548Illumina Single End PCR Primer 1 (96% over 32bp)
GCTTCAGCTTGTTTCTCCAAGAGTTTCCTAACACAGAGATAAATACATAC10.8547008547008548No Hit
GTGGCAGCCGGACAGTCGCTGGATCGACCTGAAGGCCAATCTCTGGGCCA10.8547008547008548No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCCCCATGT10.8547008547008548No Hit
ACTGTGCACTTTTTTCTAGACTCTGGGTATACAGTGGAGATCAAAGTTAA10.8547008547008548No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA10.8547008547008548No Hit
GTCCAGAATTGAGTATGTGCTTCTTGGCAGGGCGGAGGTGGCATCAGTCT10.8547008547008548No Hit
CACTAATCATGCATGGCTCTCAAGCACTTGAAAATGTTGCTAGTGTGATT10.8547008547008548No Hit
TCTACACTCTTTCCCTGCAGCTCCGTGTAGGGAAAGAGTGTAGATCTCGG10.8547008547008548Illumina Single End PCR Primer 1 (100% over 27bp)
CTTTCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGAGATCGG10.8547008547008548No Hit
TATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGCGTTG10.8547008547008548No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTATGACTTTCTTTTTGATTGGTAATGAATCACATGCCTGTCTCTTATAC10.8547008547008548No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTACTATGCGTTGTTAC10.8547008547008548No Hit
GCGCCACCGAGATCTACACACTTTCCCAACACGACGCACTTCCGATCTAT10.8547008547008548No Hit
CTTATAAGGTTTTTTTACCCTCTTATCTAGTATACCATCTTTATTTTTTT10.8547008547008548No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGT10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTGGGTGTACGGGACCACGAGATACACTCTTTCCC10.8547008547008548No Hit
GCGCAGCGCGGTGGTGGGCAGCTTGATCACGGGCTGGCCCGTGGGGCCCA10.8547008547008548No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAT10.8547008547008548Clontech Universal Primer Mix Long (95% over 21bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAGAGCGTCGTGTAG10.8547008547008548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10.8547008547008548No Hit
GAGTGTAGATCTCGGTGGTGCCGTTATCCCATGTACTCTGCGTTGATCTG10.8547008547008548No Hit
GTTTTTAAAAGTATTTTCTGTTTAAATTTTAAAAACTCTAAGGAAATTTA10.8547008547008548No Hit
GGTATCAACGCAGAGTACATGGGAACCACAGATTACATCTTCCCTACACG10.8547008547008548No Hit
ATACAGTTCCATATCATTCATCGTGGTGCAGAGGTAAATTTGCTCGTCGG10.8547008547008548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)