FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTGACTTACAATATTCAATTCACTCTGACCAAACTTCCTATGAGAA32.564102564102564No Hit
ACCCAATTCAGACCAGATCCCATATATTCATCTTTCCTCAAAATACTATT32.564102564102564No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG21.7094017094017095Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA21.7094017094017095Illumina Single End PCR Primer 1 (100% over 21bp)
GGAAGAAGCCGAAGCCCTTGGCCAGCTCGGCCGGGATGAGGTTGCGCAGG21.7094017094017095No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.7094017094017095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTTCCTGAGCTGGGTTTGAAGCATGGCTGAGCAGGAAAGTCCGAAGGGG21.7094017094017095No Hit
GCAGAGTACATGGGAAGACGGCGCACCGAGATCACACTCTTTCCCTACAC21.7094017094017095No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT21.7094017094017095Clontech SMART CDS Primer II A (100% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCT21.7094017094017095Clontech SMART CDS Primer II A (96% over 25bp)
GACTTAGGTTCTCCTGCTTGAGTTTCTGGTTGCTGTGTTGCAGAAAGCGA21.7094017094017095No Hit
GGGAAGCCTCTTGGAGTTTTCAGAAGATGAAAAGGAATGGCTGAGAATCC21.7094017094017095No Hit
GAGGTAGAGGGGTGCCGATCCCATGTACTCTGCGTTGATACCACTGCTTC21.7094017094017095Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.7094017094017095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCGTAGGGCGCGCGCGGCTCCTCGCACAGCAGCGTGACCGACCACGAGC10.8547008547008548No Hit
GTACATGGGTGATACGGCGACACCGAGATCTACACTCTTTCCCTACACGA10.8547008547008548Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGATGTATCCGGGTCTCTCCCATGT10.8547008547008548Illumina Single End PCR Primer 1 (96% over 28bp)
CATGATGCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGT10.8547008547008548No Hit
GCCAGTGATTGCTCGCCCCTTCCCCGTTCTGCCAGTCAGGCATGATCAGC10.8547008547008548No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
TCAGCCCCCCGAGCAGCTGGGATTACAAGCATCAAGCATCACGTCTGGCT10.8547008547008548No Hit
CCATAGAAGGACAGATGTTTTTAATCATTATGAAGCCCAAGTGATCTATA10.8547008547008548No Hit
TGTTTTTTTTTTCTATTTTTTTCATTGCTTTTAAAGTTTCAAAAGCTTTC10.8547008547008548No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAACTGCGTTGATACCACT10.8547008547008548No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTCGTTTTAAACAAAGGTTCCCA10.8547008547008548No Hit
CATTAACATGCTATGGATTTATGGCCAGCGCTGTGCAGTGCCCCCAGCGC10.8547008547008548No Hit
ACCCTGGCCTCAGAATTAGTCATTTCTCCAAGCAACCCTAGGAATTACCA10.8547008547008548No Hit
ACCCAATCTGAACAACAAAAAAATACACTGGGGAAAAAAAAAAGCGCAAA10.8547008547008548No Hit
GAAGCAGTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.8547008547008548No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
CGCTCCTTGTGTCGGTGGCCCAGAGATTGGCCTTCAGGTCGATCCAGCGA10.8547008547008548No Hit
GTGCCGATCCGGTCACGCGCACCTACCAGGTGAAGGTGGCTTTGCCCACC10.8547008547008548No Hit
GTGCATCGGACAGGCCACGAATCCTTCCGATCGTGTCCTTCTGGCACCGG10.8547008547008548No Hit
TCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG10.8547008547008548Illumina Single End PCR Primer 1 (100% over 22bp)
GTACATGGGAATGATACGCGACCACCGAGATCTACACTCTTTCCCTACAC10.8547008547008548Illumina Single End PCR Primer 1 (100% over 33bp)
CTTAATATATACAGAAAATCATGATATCAGTTCATAGAGCTGTCTCTTAT10.8547008547008548No Hit
CCGGGCGGCCTCGGCAAGTGCCTCGAAGCCACCATATATCCTGCCCTCGA10.8547008547008548No Hit
ATGCTGTGCTGTTCCGTACGGCAGCCACTACCCACATGTAACTTTTTAAG10.8547008547008548No Hit
CTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGGA10.8547008547008548Illumina Single End PCR Primer 1 (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCTTCCCATGTACTCTGCGTTGA10.8547008547008548Clontech Universal Primer Mix Long (95% over 21bp)
ACTCCAGTCTGGGTGACAGAACGAGACCTTGTCTCTTAACAAAAAATATA10.8547008547008548No Hit
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAA10.8547008547008548Illumina Single End PCR Primer 1 (100% over 35bp)
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG10.8547008547008548Illumina Single End PCR Primer 1 (100% over 25bp)
CTTCCGATCTGGGCCTCCAGTTCCTGAATGAGGAACTGGAGGCCCAGATC10.8547008547008548No Hit
GCTGGCTCTCTGTCTCTGTAACCCCTCTAGCCTTTCCTTTCTGGGGGACT10.8547008547008548No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA10.8547008547008548Clontech Universal Primer Mix Long (96% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATGTGATTCATTACCAATCAAAAAGAAAGTCATACCTGTCTCTTATAC10.8547008547008548No Hit
TAACAATAGAGCACTAAAAATACGAATTTTTAGTCTTTGGGACCTTCATA10.8547008547008548No Hit
GTACATGGGAAGCAGTGGTATCACGCAGAGTACATGGATTACGGCGACCA10.8547008547008548No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8547008547008548Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGTCGCGGGCCCCCGCAAAAAAAAACATTACACAAAAGACCCG10.8547008547008548No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGAA10.8547008547008548Illumina Single End PCR Primer 1 (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.8547008547008548Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACATGGGTGAACGCCCACCGAGTCTACACTCTTTC10.8547008547008548No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGATCGACCA10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA10.8547008547008548No Hit
GTACATGGGTCGGGGCCCCCCAGTAAAAAAAAAAATACCAAAACATCGCA10.8547008547008548No Hit
ACGTGGTCGTCTGGCTCGCCAGTTGAGAAGAAGAACGGAATAGCCGAGGC10.8547008547008548No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA10.8547008547008548Clontech Universal Primer Mix Long (95% over 22bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTTT10.8547008547008548No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCTG10.8547008547008548Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCACTGT10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAATGTACGGCGACCACCGAGATCTACCTCTTTCCCTACACGAC10.8547008547008548Illumina Single End PCR Primer 1 (95% over 22bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGTATACGGCGACA10.8547008547008548Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGTGTCGGACCACCGAGATTACACTTTCCTACACGACCTGT10.8547008547008548No Hit
GGTAAGATACGGCGACCACCGAGATCTACTCTTTCCCCTGTCTCTTATAC10.8547008547008548Illumina Single End PCR Primer 1 (96% over 27bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAA10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAAGAGTGTATCTCGTGGTCCCGTACACCCAGTACTCTGCGTTGATA10.8547008547008548No Hit
GCGCAGTTGATGGCGACGAACTGGCCCTTGGAGCGGCGGCTCTTCTCGTG10.8547008547008548No Hit
GTCTTGAACTCCTGACCTTGTGATCTGTCCGCCTCAGCCTCCCAAAGTGC10.8547008547008548No Hit
GTACATGGGAATACATCCATCCCTACACGGAGCTGCCGATACCAGTAAAC10.8547008547008548No Hit
TTCTTGATCCTGCCGTGCTCGTCGAACAGCCCGAGGGTGTGCTCCAGCGG10.8547008547008548No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAATTTA10.8547008547008548Clontech Universal Primer Mix Long (95% over 21bp)
CCTTTCCAATGGATCAGGGTAGATTTATGACTCATTTGCAAAGCAAAAGC10.8547008547008548No Hit
AGCTAATTTTTGTAGTTTTAGTAGAAATGGGGTTTCACCATGTTGGCCAG10.8547008547008548No Hit
GTTAATCTGGCCTTTATGGCAGGTTTGGCATTTTCAATTACAGGGTTAGG10.8547008547008548No Hit
CTACCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG10.8547008547008548No Hit
GTCCGACGAGCAAATTTACCTCTGCACCACGATGAATGATATGGAACTGT10.8547008547008548No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8547008547008548No Hit
ATCAACGCAGAGTACATGGGGAACGACCGAGATACACTCTTTCCTACACG10.8547008547008548No Hit
GTACATGGGGACGTGTGCTCTTCCGATCTCCTGATTAACAGGGCTTAACC10.8547008547008548No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA10.8547008547008548Clontech Universal Primer Mix Long (95% over 21bp)
GACCCGGCCACTGGTAGCTCCCCACTTACTTACACCTGCTGCTCTGCCAT10.8547008547008548No Hit
CAGTGGTATCAACGCAGAGTACATGGGGCGACCAGCTACTCTTCCTACAC10.8547008547008548Clontech SMARTer II A Oligonucleotide (100% over 22bp)
ATCAACGCAGAGTACATGGGATAACGGCACCACCGAGATCTACACTCCTG10.8547008547008548No Hit
GTTCACTACCTTTTCAGGTTTATTGTTTTTATTTTTGCATGAATTAAGAC10.8547008547008548No Hit
CATGGGTATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGC10.8547008547008548Illumina Single End PCR Primer 1 (100% over 43bp)
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAA10.8547008547008548No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAAG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTA10.8547008547008548No Hit
TCCTGCGCCAGCGCCCGCACGATCGCCGCCCGCCGCCGCTCGCCGCCGCT10.8547008547008548No Hit
ATAAATATATCACAAAGCCTTTTGACTGTAAAGTGTTTCTTCAGATAAAT10.8547008547008548No Hit
CTACACGACGCTCTTCCGATCTCTAAACAGGATGAGTCGGGCATACCGGA10.8547008547008548Illumina Single End PCR Primer 1 (100% over 22bp)
GCTTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.8547008547008548Clontech Universal Primer Mix Long (96% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACACTGCGTTTATACCAA10.8547008547008548No Hit
GAGTACATGGGTAGCGGACCCAGATCAATAATTACCCACACAGAGCAGCC10.8547008547008548No Hit
ATCCTCCTGTTCCTCCGCCGCGGCGAGGAGACCGAGGTCCTGCTCGGCCA10.8547008547008548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)