FastQCFastQC Report
Tue 10 May 2022
EGAF00003643410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255614
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC18350.7178793023856284No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13870.542615036735077No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13820.5406589623416558No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10710.418991135070849No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA10270.4017776804087413No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9920.38808515965479196No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC9100.35600553960268216No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT8480.3317502171242577No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA8160.31923134100636114No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT8110.3172752666129398No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC7110.27815377874451325No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7090.2773713489871447No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7000.2738504150789863No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT6930.2711119109281964No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6890.26954705141345936No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6720.2628963984758268No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6270.24529172893503487No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG6080.23785864624003378No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG5860.22925191890897997No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA5840.22846948915161142No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT5820.22768705939424289No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG5800.22690462963687436No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT5720.22377491060740023No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT5600.21908033206318903No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT5590.21868911718450476No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT5570.21790668742713623No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT5270.20617024106660825No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG5240.20499659643055546No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC5050.1975635137355544No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT4860.19013043104055333No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG4850.1897392161618691No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA4690.18347977810292082No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG4680.18308856322423656No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC4670.18269734834555226No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC4640.18152370370949947No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG4590.17956762931607814No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT4590.17956762931607814No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA4550.17800276980134108No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA4390.1717433317423928No Hit
GCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCT4300.16822239783423443No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT4210.16470146392607604No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG4090.16000688538186483No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG4080.15961567050318057No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC4040.1580508109884435No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA4030.15765959610975924No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG3920.1533562324442323No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG3920.1533562324442323No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG3900.1525738026868638No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA3860.15100894317212674No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA3760.14709679438528406No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA3740.14631436462791553No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG3640.14240221584107285No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC3620.14161978608370432No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT3540.13849006705423023No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC3530.13809885217554593No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA3430.13418670338870328No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG3410.13340427363133475No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG3400.1330130587526505No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC3370.13183941411659766No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC3360.1314481992379134No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA3310.12949212484449207No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT3300.1291009099658078No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG3300.1291009099658078No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG3300.1291009099658078No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA3260.12753605045107078No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT3230.12636240581501795No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG3210.12557997605764942No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA3180.12440633142159663No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA3170.12401511654291236No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA3170.12401511654291236No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA3170.12401511654291236No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC3140.12284147190685957No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA3130.1224502570281753No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA3120.12205904214949102No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT3120.12205904214949102No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG3120.12205904214949102No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC3080.12049418263475396No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC3080.12049418263475396No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT3060.11971175287738543No Hit
ATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGA3050.11932053799870115No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC3000.11736446360527983No Hit
ATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAA2910.11384352969712144No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA2880.11266988506106865No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT2850.11149624042501585No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG2850.11149624042501585No Hit
GCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCC2850.11149624042501585No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC2840.11110502554633157No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC2840.11110502554633157No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG2820.11032259578896304No Hit
CAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTT2810.10993138091027878No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT2780.10875773627422598No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA2770.10836652139554172No Hit
ATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCA2760.10797530651685744No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC2740.10719287675948891No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC2720.10641044700212038No Hit
ATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAAC2710.10601923212343611No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG2710.10601923212343611No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT2700.10562801724475185No Hit
GAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGC2690.10523680236606758No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT2680.1048455874873833No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG2680.1048455874873833No Hit
ATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTA2670.10445437260869904No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT2670.10445437260869904No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG2660.1040631577300148No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA2660.1040631577300148No Hit
GGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAG2650.10367194285133054No Hit
GGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACAC2650.10367194285133054No Hit
ACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTCAA2640.10328072797264626No Hit
AGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTC2640.10328072797264626No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG2640.10328072797264626No Hit
ATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACA2620.10249829821527773No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA2610.10210708333659346No Hit
AACATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCC2580.10093343870054067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)