FastQCFastQC Report
Tue 10 May 2022
EGAF00003643431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174222
Sequences flagged as poor quality0
Sequence length140
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG22721.3040832960246123No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC18271.0486620518648622No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG18271.0486620518648622No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG18231.0463661305690442No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT17090.9809323736382316No Hit
AGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT14420.8276796271423815No Hit
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG13750.7892229454374304No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA13500.7748734373385681No Hit
GTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCT12320.7071437591119376No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT12060.6922202706891207No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG11520.6612253331955781No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA11190.6422839825050797No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC10080.5785721665461309No Hit
CTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTT9640.553317032292133No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC9630.5527430519681785No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT9510.5458552880807246No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA9360.5372455832214071No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG8280.4752557082343217No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG8240.4729597869385037No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT8120.4660720230510498No Hit
CTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTC7490.42991126264191665No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT7440.4270413610221442No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT7270.4172836955149178No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC7160.4109699119514183No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC7070.4058040890358278No Hit
GCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCC6920.39719438417651043No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC6900.3960464235286014No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA6880.3948984628806924No Hit
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT6760.38801069899323853No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA6710.38514079737346607No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC6550.3759571121901941No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG6170.3541458598799233No Hit
GCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCC6150.3529978992320143No Hit
CTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCT6100.35012799761224184No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA6040.34668411566851487No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA6020.34553615502060586No Hit
ACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC5980.3432402337247879No Hit
GTATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGA5840.33520450918942496No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG5810.3334825682175615No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA5600.3214289814145171No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC5540.3179850994707901No Hit
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG5460.3133932568791542No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT5410.31052335525938174No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT5330.30593151266774576No Hit
CCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGC5300.30420957169588225No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT5300.30420957169588225No Hit
GGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGC5240.3007656897521553No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG5170.29674782748447387No Hit
CACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCT5160.29617384716051937No Hit
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT5150.29559986683656486No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGC4930.282972299709566No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC4890.280676378413748No Hit
TTTGTGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC4770.27378861452629405No Hit
GCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACAC4750.27264065387838504No Hit
CCCCGAGCCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTG4730.2714926932304761No Hit
GGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAG4700.2697707522586126No Hit
GTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGC4650.2669008506388401No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC4640.2663268703148856No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC4640.2663268703148856No Hit
CAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGG4600.2640309490190676No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT4580.26288298837115864No Hit
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC4570.26230900804720414No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC4550.26116104739929513No Hit
CCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAG4520.2594391064274316No Hit
TCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTA4490.25771716545556816No Hit
TCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACT4460.25599522448370465No Hit
CTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGT4410.2531253228639322No Hit
GTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATC4290.24623755897647828No Hit
CTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGA4220.2422196967087968No Hit
GCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGA4200.24107173606088783No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC4110.23590591314529735No Hit
GCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCC4110.23590591314529735No Hit
CCTCATAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGA4080.2341839721734339No Hit
GGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGG4010.23016610990575243No Hit
TAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTC3990.22901814925784342No Hit
GCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTT3940.22614824763807095No Hit
CTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCG3900.223852326342253No Hit
GGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTGTGTCCATGAG3900.223852326342253No Hit
TTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGA3880.222704365694344No Hit
GTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGG3870.22213038537038948No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC3850.22098242472248053No Hit
AGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACACCCTCCAGTTA3840.22040844439852603No Hit
AGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT3840.22040844439852603No Hit
CTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGC3770.21639058213084456No Hit
CTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGG3750.21524262148293555No Hit
TTTGCGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC3710.2129467001871176No Hit
GGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATG3660.21007679856734512No Hit
GCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAAT3600.20663291662361816No Hit
TATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAA3580.20548495597570915No Hit
TGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGAT3560.20433699532780014No Hit
CTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATG3500.20089311338407317No Hit
GAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTT3450.1980232117643007No Hit
ACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGA3430.19687525111639176No Hit
GCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAG3370.19343136917266476No Hit
GAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG3350.19228340852475578No Hit
CTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCAT3210.18424768398939284No Hit
CTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTT3170.18195176269357485No Hit
CTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATG3140.18022982172171137No Hit
ACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC3130.17965584139775687No Hit
GGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGG3100.17793390042589338No Hit
GGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGC3090.1773599201019389No Hit
TGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGA3070.1762119594540299No Hit
GACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCA3040.17449001848216641No Hit
GTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGC2940.1687502152426215No Hit
GGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGA2940.1687502152426215No Hit
CCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTC2910.167028274270758No Hit
GTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTG2830.16243643167912203No Hit
CTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCC2830.16243643167912203No Hit
CTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCT2800.16071449070725854No Hit
TAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGAAAGCT2770.15899254973539506No Hit
CAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGG2740.15727060876353158No Hit
CCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGG2720.1561226481156226No Hit
CTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCC2720.1561226481156226No Hit
TCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCC2600.14923488422816866No Hit
GGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGG2580.14808692358025968No Hit
GCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCAC2580.14808692358025968No Hit
CTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCC2550.1463649826083962No Hit
CCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGC2530.1452170219604872No Hit
GCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGG2530.1452170219604872No Hit
GTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATCAG2510.1440690613125782No Hit
CTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCAGCTCAGCCACAC2410.13832925807303326No Hit
CTCAGAGACGGAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATG2380.13660731710116977No Hit
CTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAG2330.13373741548139728No Hit
CTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATCAGGGCTGAAAAGTT2260.1297195532137158No Hit
CTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTC2240.12857159256580683No Hit
ACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTG2230.12799761224185233No Hit
TTTTCGAGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGG2210.12684965159394335No Hit
GCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACC2210.12684965159394335No Hit
CCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGC2190.12570169094603437No Hit
GTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGC2180.12512771062207986No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA2180.12512771062207986No Hit
GCTTTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCT2180.12512771062207986No Hit
CCCTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTG2180.12512771062207986No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG2160.12397974997417088No Hit
TGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGGGTGCCGAGGGGG2150.1234057696502164No Hit
AGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACACCCTCC2130.1222578090023074No Hit
CTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGGCCC2100.12053586803044392No Hit
ACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTG2100.12053586803044392No Hit
CACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGAC2090.11996188770648941No Hit
GCCTGGGGACCCAGCTCAGCCACACACTTCTGGGAGCCCTCTATGAGGTG2090.11996188770648941No Hit
AGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTT2080.11938790738253492No Hit
CCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTGGCCCTGT2070.11881392705858043No Hit
CCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGG2000.11479606479089896No Hit
GTATCAACGCAGAGTACATGGGAGCCCACCAGACTCAGAGACGGAACCAG2000.11479606479089896No Hit
TTTGAGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC2000.11479606479089896No Hit
GAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCT1950.1119261631711265No Hit
CCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCT1930.11077820252321749No Hit
GGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACA1920.11020422219926301No Hit
ACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGAAAGCTCG1910.10963024187530851No Hit
CATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTG1900.109056261551354No Hit
GACTCAGAGACGGAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCA1900.109056261551354No Hit
GCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGG1900.109056261551354No Hit
GGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTT1900.109056261551354No Hit
GCCCCTGCCTCCCTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGC1890.10848228122739953No Hit
GTACATGGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGG1860.10676034025553605No Hit
ATCGAAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGA1750.10044655669203659No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)