FastQCFastQC Report
Tue 10 May 2022
EGAF00003643432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174222
Sequences flagged as poor quality0
Sequence length140
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG21341.2248740113188918No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC18121.0400523470055445No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT15430.8856516398617855No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG14390.825957686170518No Hit
AGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT13530.7765953783104315No Hit
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG13450.7720035357187955No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG12880.7392866572533894No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA12420.7128835623514825No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT12210.7008299755484382No Hit
GTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCT11290.6480237857446247No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG10550.605549241771992No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC9810.5630746977993595No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG9270.5320797603058167No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA9260.5315057799818622No Hit
CTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTT9170.5263399570662718No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC9000.5165822915590453No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA8950.5137123899392729No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT8520.4890312360092296No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA8180.4695159049947768No Hit
CTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTC8130.46664600337500434No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC8040.46148018045941386No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC7260.4167097151909632No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT7130.40924797097955484No Hit
GCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCC7010.4023602070921009No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG6830.39202856126091995No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT6720.3857147776974206No Hit
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT6660.38227089575369355No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA6380.36619944668296767No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC6300.36160760409133175No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC6210.35644178117574127No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA5890.3380744108091975No Hit
GCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCC5860.33635246983733397No Hit
ACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC5680.32602082400615306No Hit
CTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCT5590.32085500109056264No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT5560.31913306011869913No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT5550.3185590797947446No Hit
GTATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGA5460.3133932568791542No Hit
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG5330.30593151266774576No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA5260.3019136504000643No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG5160.29617384716051937No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC5160.29617384716051937No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT5150.29559986683656486No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC5110.2933039455407469No Hit
GGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGC5090.2921559848928379No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC4980.28584220132933846No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT4930.282972299709566No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT4920.2823983193856115No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG4890.280676378413748No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC4880.2801023980897935No Hit
CTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGT4850.27838045711793No Hit
AGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACACCCTCCAGTTA4840.2778064767939755No Hit
GCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACAC4800.2755105554981575No Hit
CCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAG4720.2709187129065216No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG4670.26804881128674907No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC4650.2669008506388401No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC4630.2657528899909311No Hit
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT4620.2651789096669766No Hit
TCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACT4600.2640309490190676No Hit
CACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCT4600.2640309490190676No Hit
CCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGC4410.2531253228639322No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA4290.24623755897647828No Hit
CCTCATAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGA4290.24623755897647828No Hit
CAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGG4150.23820183444111537No Hit
TCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTA4060.2330360115255249No Hit
CTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGA4040.23188805087761588No Hit
GTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATC4030.23131407055366143No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGC4020.23074009022970693No Hit
TGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGAT3980.2284441689338889No Hit
GCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGA3960.22729620828597996No Hit
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC3950.22672222796202546No Hit
GTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGC3930.22557426731411645No Hit
TAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTC3900.223852326342253No Hit
CCCCGAGCCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTG3900.223852326342253No Hit
GGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGG3860.22155640504643498No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC3830.21983446407457152No Hit
CTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCG3810.21868650342666252No Hit
GCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCC3790.21753854277875356No Hit
CTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGC3760.21581660180689005No Hit
TTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGA3750.21524262148293555No Hit
AGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT3720.2135206805110721No Hit
GGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAG3630.2083548575954816No Hit
GAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTT3630.2083548575954816No Hit
GTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGG3590.20605893629966365No Hit
GGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTGTGTCCATGAG3560.20433699532780014No Hit
GCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTT3520.20204107403198218No Hit
CTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGG3440.1974492314403462No Hit
CTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCC3410.19572729046848275No Hit
CTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATG3390.19457932982057377No Hit
CTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATG3330.19113544787684678No Hit
ACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC3290.18883952658102882No Hit
GAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG3230.18539564463730182No Hit
GGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATG3190.18309972334148383No Hit
AGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACACCCTCC3150.18080380204566587No Hit
TTTGTGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC3080.1767859397779844No Hit
CTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTT3080.1767859397779844No Hit
GCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAG3070.1762119594540299No Hit
ACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGA2920.16760225459471248No Hit
TAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGAAAGCT2880.16530633329889452No Hit
GCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAAT2830.16243643167912203No Hit
GGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGC2830.16243643167912203No Hit
GGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGG2800.16071449070725854No Hit
CTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCC2780.15956653005934957No Hit
CCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTC2770.15899254973539506No Hit
CTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCC2760.15841856941144059No Hit
TATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAA2730.1566966284395771No Hit
CCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGG2700.15497468746771362No Hit
CTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTC2700.15497468746771362No Hit
CTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAG2680.1538267268198046No Hit
TGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGA2680.1538267268198046No Hit
TTTGCGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC2680.1538267268198046No Hit
GTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTG2650.15210478584794113No Hit
GCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCAC2630.15095682520003215No Hit
CCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGC2620.15038284487607764No Hit
GACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCA2600.14923488422816866No Hit
CCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGG2580.14808692358025968No Hit
TCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCC2530.1452170219604872No Hit
CAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGG2470.14177314001676022No Hit
CCCTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTG2460.14119915969280575No Hit
GCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGG2450.14062517936885124No Hit
CTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCAT2420.13890323839698776No Hit
GCTTTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCT2400.13775527774907875No Hit
GTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGC2350.1348853761293063No Hit
AGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTT2310.1325894548334883No Hit
CACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGAC2310.1325894548334883No Hit
ACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTG2300.1320154745095338No Hit
CCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGC2290.13144149418557932No Hit
CATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTG2270.13029353353767031No Hit
CTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCAGCTCAGCCACAC2260.1297195532137158No Hit
CCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGC2240.12857159256580683No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA2230.12799761224185233No Hit
CTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCT2210.12684965159394335No Hit
CTCAGAGACGGAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATG2210.12684965159394335No Hit
GGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGA2210.12684965159394335No Hit
GAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCC2160.12397974997417088No Hit
GTCCATGAGGAGGGTTTCGATGACACGCTGAAAGCTCGGGCAGATCTCTG2110.1211098483543984No Hit
GTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATCAG2090.11996188770648941No Hit
CTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGC2050.11766596641067144No Hit
CTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATCAGGGCTGAAAAGTT2040.11709198608671695No Hit
GCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTC2030.11651800576276244No Hit
CCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTGGCCCTGT2000.11479606479089896No Hit
GTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGC1980.11364810414298998No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG1970.11307412381903548No Hit
GCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCG1960.11250014349508097No Hit
CCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCA1950.1119261631711265No Hit
ATCGAAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGA1930.11077820252321749No Hit
ATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTT1900.109056261551354No Hit
ACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGAAAGCTCG1890.10848228122739953No Hit
GTATCAACGCAGAGTACATGGGACTAGCCCACCAGACTCAGAGACGGAAC1880.10790830090344503No Hit
GCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGG1880.10790830090344503No Hit
GGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGG1870.10733432057949052No Hit
GACTCAGAGACGGAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCA1850.10618635993158154No Hit
GCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACC1840.10561237960762704No Hit
CCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAG1830.10503839928367256No Hit
CTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCC1810.10389043863576355No Hit
GCCTGGGGACCCAGCTCAGCCACACACTTCTGGGAGCCCTCTATGAGGTG1810.10389043863576355No Hit
CTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACACCCTCCAGTTATG1770.1015945173399456No Hit
GGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTT1760.10102053701599109No Hit
GGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACA1760.10102053701599109No Hit
TCGAAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAA1760.10102053701599109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)