FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003643439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC29.090909090909092Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTCACGCCCGCGCCGGCCTGCAGCAGCGTGGGGTCGAGCACCCACAG29.090909090909092No Hit
CTTCTCGACTTCGTCGATCACCTGTCGCAGTCGCTGCTCGAACTCGCCCT29.090909090909092No Hit
GTTTGAGGCCCCGAGCTGGAGGGTTCTTATCTGCCTAGTACGCGCGGGGC14.545454545454546No Hit
TCAACGCAGAGTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTAT14.545454545454546No Hit
TCTTTCTGCTCCGCTGAGAAGTACACCTTTTTGCTAGCATCTCGCACGGC14.545454545454546No Hit
TTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCG14.545454545454546No Hit
CGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTCCC14.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
GTGGAAAGCTGTGTGTTGATTTCATGAAAGCGATTTCTGATATGGCGGCG14.545454545454546No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG14.545454545454546No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA14.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
CCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGC14.545454545454546No Hit
AAACAGGATGAGTCCGGTATGCCCATGTACTCTGCGTTGATACCCTGTCT14.545454545454546No Hit
TCTTTCTGCTCCGCTGAGACGTACACCTTTTTGCTAGCATCTCGCACGGC14.545454545454546No Hit
GACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACT14.545454545454546No Hit
ATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGC14.545454545454546No Hit
ACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCC14.545454545454546No Hit
GTGTAGGGAAAGAGTGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAATTA14.545454545454546No Hit
CACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCAC14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)