FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003643440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length140
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAGATCATCGACATCCTCATGCGCCGCCGCCAGAACAACCCGCTGCT29.090909090909092No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC29.090909090909092Clontech SMART CDS Primer II A (96% over 26bp)
AGAACAACCCGCTGCTGACCGGCGAGGCCGGCGTCGGCAAGACGGCGGTG29.090909090909092No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA29.090909090909092No Hit
GGCGAGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAAT29.090909090909092No Hit
TTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAA14.545454545454546No Hit
CACCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGACCTTGGAGTTT14.545454545454546No Hit
GGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACAC14.545454545454546No Hit
GGTATCAACGCAGAGTACATGGGCATACCGGACTCATCCTGTTTCTGTCT14.545454545454546No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCCCAAAACAATTT14.545454545454546No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT14.545454545454546Clontech Universal Primer Mix Long (100% over 23bp)
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG14.545454545454546No Hit
ATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGGAAAAGTCACA14.545454545454546No Hit
ATATGTAGCGGCGTCAGCCCTGTTCCGTTTTTGTGTGACGCTGGTCAGTA14.545454545454546No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA14.545454545454546Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG14.545454545454546No Hit
CTGAAGGCTATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATC14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)