FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA35.084745762711865Illumina Single End PCR Primer 1 (100% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23.389830508474576Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTA23.389830508474576Clontech Universal Primer Mix Long (96% over 27bp)
TAGTAGACAGGGTTTCGCCATATTTGCCAGGCTAGTCTCGAACTCCTGAC23.389830508474576No Hit
ACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGTAGGG11.694915254237288No Hit
GTCCAAAATCTATGAACAAGGCCTTGGCGCGGATAAAAATCCAATCATGG11.694915254237288No Hit
TCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAAAAAAAAAAAA11.694915254237288Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG11.694915254237288Clontech Universal Primer Mix Long (96% over 26bp)
CTGTTCTGGTATCGACAAGACCTGGGACAGATCGGAAGAGCGTCGTGTAG11.694915254237288Illumina Single End PCR Primer 1 (100% over 22bp)
GTACATGGGGCCGTCTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTG11.694915254237288Illumina Single End PCR Primer 1 (96% over 25bp)
CGTTCTTCTTCTCCCATGTACTCTGCGTTGATGCGTTGATACCACTGCTT11.694915254237288No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAGTAAC11.694915254237288Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT11.694915254237288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGT11.694915254237288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.694915254237288No Hit
GAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGCTGTCTC11.694915254237288Clontech Universal Primer Mix Long (96% over 27bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAACACGGGGTC11.694915254237288No Hit
GGAGTGTACTGGAGTGGAAAGGACTGGAATGTAGTGGAGTGTAATGGAAT11.694915254237288No Hit
GCCTTAGGAAACAAATATAATTCTGAGGCAGTTGCAGTTGTCCTCAAGGA11.694915254237288No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.694915254237288Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGA11.694915254237288Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAGGACC11.694915254237288Clontech Universal Primer Mix Long (95% over 21bp)
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG11.694915254237288No Hit
GTGGAGAGCGCGCGGCAGTGCAGATTGCCGTCGACCTCGGCTCTACGGAT11.694915254237288No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAATTAAC11.694915254237288Clontech Universal Primer Mix Long (95% over 21bp)
GTGTAGATCTCTGTCGGTACTTCCCATGTACTCTGCGTTGATACCACTGC11.694915254237288Clontech SMART CDS Primer II A (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGGCAGTGGTA11.694915254237288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCGAATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGG11.694915254237288Illumina Single End PCR Primer 1 (100% over 37bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA11.694915254237288No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAGTACC11.694915254237288Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGATACTACTGCTTAACCCATGTACTCTGCGTTGATACCACTGCTT11.694915254237288Clontech SMART CDS Primer II A (96% over 26bp)
GTGTAGATCTCGGTGTGCCGTACACCCATGTACTCTGCGTTGATGCCACT11.694915254237288Clontech SMARTer II A Oligonucleotide (95% over 21bp)
CCCATGTACTCTGCGTTGATACAACTGCTTCCAGTCTCTTATACACATCT11.694915254237288Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAATTAAAA11.694915254237288No Hit
CAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATA11.694915254237288No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTAT11.694915254237288Clontech SMART CDS Primer II A (96% over 25bp)
ATCAAAAGTGTATTTAGAGATCAAAATACTGTAAGCAATTCATTTTGCAG11.694915254237288No Hit
GTATGTCCTGTGAACTTCTTCAGAAATTCTGCCTTTTTCTGGTGGGCATC11.694915254237288No Hit
TTCCACTACCGGCGAGAACGCAATCACATCGACCTTCTGCGCGATGTACG11.694915254237288No Hit
GTATCAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACACTGCGT11.694915254237288No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTGTC11.694915254237288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTAT11.694915254237288No Hit
GTGATGCCCCAGCGCTCCAGTTCGATCTTCTCGTCGAGGATGGGTTCGCG11.694915254237288No Hit
GTCATGAAATCTTAGGTTGGACCTTTGGTTTGGAAACTTTGTCTTCCTCT11.694915254237288No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.694915254237288Clontech Universal Primer Mix Long (95% over 21bp)
CCCTATGCGCTGATCGTGCTCGATCTGGGATTGCCGGACATCGACGGGAT11.694915254237288No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGTATCA11.694915254237288Clontech SMART CDS Primer II A (95% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGAACATGGGAAGCAGTGGTA11.694915254237288Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGGTGGGCTTCGATCCCGTTTTCGGGGCCCGGCCGCTCAAGCGCGCGATA11.694915254237288No Hit
GTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGG11.694915254237288No Hit
TTTTTTTTTCTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCCTGTC11.694915254237288Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT11.694915254237288Clontech SMART CDS Primer II A (100% over 26bp)
TGGCATGAAATCGTCTCATTGGCAAAGGAAGTCGAAGCTGCCGGCGCCAC11.694915254237288No Hit
ACATAATACCACAAAAATAAAAATAGAAGTAGCTTCCATGTGTCCAAAAT11.694915254237288No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)