FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGGTCGGCGACCCCGAGATCTCACTCTTTCCCTACACGACGC35.084745762711865Illumina Single End Sequencing Primer (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35.084745762711865Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA35.084745762711865Clontech Universal Primer Mix Long (95% over 21bp)
ACATGGAGAAACCCCATCTCTACTAAAAATACAAAAATTTGGCTGGCCGT23.389830508474576No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCCATGTACCTGTCTCTTA23.389830508474576Clontech Universal Primer Mix Long (96% over 27bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGTCGAT11.694915254237288Illumina Single End PCR Primer 1 (100% over 32bp)
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT11.694915254237288No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCT11.694915254237288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATGGGAAGCAG11.694915254237288No Hit
GTGTAAATCATCCACCAGGCTGCGGCCATGATTGGATTTTTATCCGCGCC11.694915254237288No Hit
CACTGAGCTAAAGGCCCTGGAAACTTCTGGGAGGGAGCCAGGTGAGCAAA11.694915254237288No Hit
GCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC11.694915254237288No Hit
CGCCCGGCTCCAGCACCGGGTCCACATGTACCGCAATCGTGCTCTTGGGC11.694915254237288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT11.694915254237288No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCCATGTACTCTGCGTTGATA11.694915254237288Clontech Universal Primer Mix Long (96% over 27bp)
GCTGACAAGTCGTTCACGATGGGCTTTGCCCAGGTGGGTGCCGAGAGCGA11.694915254237288No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAGAAAAAAAAACTGTC11.694915254237288Clontech Universal Primer Mix Long (95% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTAT11.694915254237288Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAGAATGCGTTTTA11.694915254237288No Hit
CTTAAAGAGAACCAGGATGCTGGATAAGCCTGACTTTTAGGGCTTATCTC11.694915254237288No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.694915254237288Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11.694915254237288No Hit
CCATGATCCAGGCCGGTGATCCGATCGGCAACTGCATCGCGTGCGCTGAG11.694915254237288No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT11.694915254237288Clontech Universal Primer Mix Long (96% over 26bp)
GGAATGTCGACGTGCTTCTCGAGCTTGCTCGTGATGTCCACGAAGGCTTC11.694915254237288No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGATCATCTTTCC11.694915254237288Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTGCGTTTTTAACAAAGATAACCCATTT11.694915254237288No Hit
ATGTGGGATACAAGACGTCAGGGTGAGGCTTGCGCGAAGCCTCCCGCAGC11.694915254237288No Hit
CTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGCGTCGTGTAGGGAA11.694915254237288No Hit
GCCGTGTCGCTGTTCGCGCGCATGAAGCCCGAAGAACTGTCGGTGCTCAA11.694915254237288No Hit
ATTCACATGTCTTTCAGTAAAAAATCTTTTGAGGAACCCAGACTTTTGAA11.694915254237288No Hit
TCAGAGTCTAGTGAAGGACCGTCATGTGAACATGGAAATATGACACACAG11.694915254237288No Hit
GTACATGGGTTAAGCAGTAGTATCAACGCAGAGTACATGGGAAGCAGTGG11.694915254237288Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACAGCGTCCCAAA11.694915254237288No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.694915254237288Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA11.694915254237288Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACTGCTTCCCAC11.694915254237288No Hit
GTGAATATGTCAGCCAAGCAAAGAGCAGCCCAGATAAAAGTAACAGTGAG11.694915254237288No Hit
TTCCATGCCTTTCCATTACTTTCCATTCAATTCCACTCCAATCCATTCCA11.694915254237288No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGTACCGACA11.694915254237288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG11.694915254237288No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTACCACAGCGTCCCCC11.694915254237288No Hit
TTTTAGCGTTGATACCACTGCCTCCCATGTACTCTGCGTTGATACCACTG11.694915254237288Clontech SMART CDS Primer II A (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTAACAAGGGTTACCCC11.694915254237288No Hit
GTATCACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.694915254237288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAGACGTGTGCTCTTCCGATCTTTCTATTAACAATTGTTAACATAGA11.694915254237288Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT11.694915254237288Clontech SMART CDS Primer II A (100% over 26bp)
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAGTACTCCTGTC11.694915254237288No Hit
GTACATGGGAATGATACGGCGACCACCGAATCTACACTCTTTCCCTACAC11.694915254237288Illumina Single End PCR Primer 1 (95% over 22bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCCATGTACTCCTGTCTC11.694915254237288Clontech Universal Primer Mix Long (96% over 27bp)
GTACATGGGCAGACGTGTGCTCTTCCGATCTGGGAATTAACGCTACTTAA11.694915254237288Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)