FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTACATGCTGAGCCTTCACCATTTAAGGAAGGGCATCCCCCCCTATGC34.225352112676056No Hit
CCCATCGGCTCGAACTCACGCCCGTGGGCGCCGAGGTGCTCGCGCGCACC22.8169014084507045No Hit
GTTTTTCAATTAGTCCACAGGTATTTATTGAGAACATACTACAGTCTCAC22.8169014084507045No Hit
GCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGA22.8169014084507045No Hit
ATATAAAGCCTGGAAGGCAGCTGGCTCCATCTGCAGAGCACTCTCCAACT22.8169014084507045No Hit
GCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAAT22.8169014084507045No Hit
GGCATGAGCTTCTCGATCCAGCCGGTGCCGGTCGCCTGCACGAGCACGCC22.8169014084507045No Hit
CGTTTATTGAGAGGGGCCGGGGGAAGGGGATGGACGGTCCTCCCCGCGGC22.8169014084507045No Hit
GCTTGGTCTGAGCAAATTGCTCAGCCTGAATGGCGATATCCGCAGCGGTA22.8169014084507045No Hit
CCTTCAAGCCCAGATCGCGGAACTGTTGCGCAGGGAGTTGCGCTTCGATG22.8169014084507045No Hit
CCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT22.8169014084507045No Hit
GTGCATGGGGCAGAATTCAGGATGGTTCCAAGGATGGAGCTTCCAGTTGT22.8169014084507045No Hit
AAAGGTGCCGCCGGCCGGGGAAAGCTACTTCTCAAAGGGGCCTCCAGGCC11.4084507042253522No Hit
CAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCC11.4084507042253522No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.4084507042253522No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA11.4084507042253522No Hit
CAGTATGATATTGGCTGTGGGTTTGTCATAGATAGCTCTGATTATTTTGA11.4084507042253522No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.4084507042253522Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCAAGTGGTCTGTAGTTTTCTTTCCTTGCAGTGTCTTTGGTTACTGAA11.4084507042253522No Hit
AAAGAATTGGACAAAATGCACAAACAAAGCAAGGAAGGAATGAAGGGTTT11.4084507042253522No Hit
GTCGCATGTTCCAGACGTCCAAAACGGGGTCCTTGTCATAACCATGGGAG11.4084507042253522No Hit
CCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATC11.4084507042253522No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTA11.4084507042253522No Hit
GTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTGCGCCCT11.4084507042253522No Hit
CAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAA11.4084507042253522No Hit
TTGCTGGAGACTGTCCTCATTCTCCTCATAGCGGTGAAACGATCGTGCGT11.4084507042253522No Hit
GCGTGAGCTGGACCTCAGCAACAACTGCCTGGGGGACGCCGGCATCCTGC11.4084507042253522No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTT11.4084507042253522No Hit
GGCGGGCGGGCCCTGCCCGGCTCGCCGCGCTAGCCCTGCTGACCTGCAGC11.4084507042253522No Hit
AAGATATACAGATAACTAGAGTTCAAACCGAGAACCAAATTTGAGTCAGC11.4084507042253522No Hit
CTTCAAAAATGAAGTCCCAACTGGGAGCAGTGGCTCATGCCTGTAATCCC11.4084507042253522No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATATTAAT11.4084507042253522No Hit
TGCCCGTGTCGGCCGTCTCCAGCCCCGCGATGATGCGCAGCAGCGTGGTC11.4084507042253522No Hit
GGAGCAGATACGGTATTTACATGAGGAATTTCCAGAGTCCTGGTCAGTTC11.4084507042253522No Hit
GGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATCTCGTGTCGG11.4084507042253522Illumina Single End PCR Primer 1 (97% over 34bp)
GAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAATAAAT11.4084507042253522No Hit
GAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAAT11.4084507042253522No Hit
AAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTT11.4084507042253522No Hit
GCCAGGATGGGTTCGACGATCTGAGCGGCAACCTCGGCGGCAGCGCCCAA11.4084507042253522No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.4084507042253522Clontech Universal Primer Mix Long (96% over 26bp)
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT11.4084507042253522No Hit
CTTTTTCACCATAGGCCTCAATGCACTCCCAAATGTCCATTCGCAGAATG11.4084507042253522No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA11.4084507042253522No Hit
GACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGAT11.4084507042253522No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGA11.4084507042253522No Hit
GGACAGAAACCTCCCGTGGAGCAGAAGGGCCTCACGATCCTTCTGACCTT11.4084507042253522No Hit
ATCCTGCCCAAGAACAACCCACCCACCATCGTGCATGACAAAGTGCTCAA11.4084507042253522No Hit
GCCAAGGTCCCCACCACGGGGGTCCCCAGTGCACCCCAGCTCCGGGGCAG11.4084507042253522No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG11.4084507042253522No Hit
TTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGT11.4084507042253522No Hit
GCGCAGGAGGGCGAACACGGCATCGACGACATTCATCGTCGGGTGGGGGG11.4084507042253522No Hit
CGTATCGGGTCGCCGAGAGGAGTGCGACGCGGTCGCCTGCCTGCACGCCA11.4084507042253522No Hit
GGTTATCCCGGCGGAAACTGACATTTTAGCCGGCGTGCAGGCGGACATTA11.4084507042253522No Hit
AGCGTCAGCACTTCCGGTGTGTAGATCGGGAGATGGAAGTGACATTTCTC11.4084507042253522No Hit
GCGCAGGAGGGCGAACACGGCATCGACGACATTCATCTTCGGGTGGAGGG11.4084507042253522No Hit
CCGCTACGCCCTACAGATGGAGCAGCTCAACGGGATCCTGCTGCACCTTG11.4084507042253522No Hit
GGAGAGCGAAAGGTTCACGTCCCAGTCCACTAAGACAGCACCTGTGGGCG11.4084507042253522No Hit
TCGGAAGAGCGTCGTGTAGGGAAAGAGTATCTCGCCCCCATGTACCTGTC11.4084507042253522Illumina Single End PCR Primer 1 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)