FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG100.328515111695138No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG90.2956636005256242No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT70.22996057818659657No Hit
GGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCT60.19710906701708278No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC60.19710906701708278No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT60.19710906701708278No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC60.19710906701708278No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT50.164257555847569No Hit
TCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAG50.164257555847569No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGC50.164257555847569No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT50.164257555847569No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC50.164257555847569No Hit
GTACATGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCA50.164257555847569No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT50.164257555847569No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.164257555847569No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40.1314060446780552Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC40.1314060446780552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAT40.1314060446780552No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40.1314060446780552Clontech Universal Primer Mix Long (96% over 26bp)
GGAGTTCAAGACTGCAGTGCACACTGATGGCACCTGTAAGTAGCCACTGC40.1314060446780552No Hit
GATCAATTACAGTTAGGCCGCTGACACATTAGTAACGTCGGCAAGCACTT40.1314060446780552No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT40.1314060446780552No Hit
GTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCC40.1314060446780552No Hit
GTGGTATCAACGCAGAGTACTGGGAATTCCTCGTTCATGGGGAATAATTG40.1314060446780552Clontech SMART CDS Primer II A (100% over 21bp)
CGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGT40.1314060446780552No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT40.1314060446780552No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG40.1314060446780552No Hit
TAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGAC40.1314060446780552No Hit
TCACAGCACTGTCTGGCTGTTGCTGGTGTTTCTCCACCCACTCCTGTCTC40.1314060446780552No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT40.1314060446780552No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT40.1314060446780552No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG40.1314060446780552No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA40.1314060446780552No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG40.1314060446780552No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA40.1314060446780552No Hit
CAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATT40.1314060446780552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1314060446780552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)