FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA153.640776699029126Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT92.1844660194174756Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG71.6990291262135921Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT40.9708737864077669Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40.9708737864077669Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT40.9708737864077669Clontech SMART CDS Primer II A (100% over 26bp)
CTTCCTACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCT30.7281553398058253Illumina Single End PCR Primer 1 (100% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT30.7281553398058253Clontech Universal Primer Mix Long (96% over 26bp)
CCTGCTGTGGGATAACTGAAAATCTTTTTATGGTAAACCATATGGGAACA30.7281553398058253No Hit
CGATTACACTCTTTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAA30.7281553398058253No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG30.7281553398058253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT30.7281553398058253Clontech Universal Primer Mix Long (96% over 26bp)
GCACTCATAGTGTTTATTACTTTTTACCTTGTATTGTTAATTCATCAAAC30.7281553398058253No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA30.7281553398058253Clontech Universal Primer Mix Long (95% over 22bp)
GAGGCAAACGCAGTCATCCTCGAGATCACTTTACCTTTTAGGCAAACCAG30.7281553398058253No Hit
GAATATTGATCTTGCATATGTCTCTGAAGGTGTTGCCAAAGGAGATTAGC30.7281553398058253No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA30.7281553398058253Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG30.7281553398058253Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30.7281553398058253Clontech Universal Primer Mix Long (96% over 26bp)
GTCATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT30.7281553398058253Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.7281553398058253No Hit
GGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.7281553398058253Clontech Universal Primer Mix Long (95% over 23bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30.7281553398058253Clontech Universal Primer Mix Long (96% over 25bp)
TCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAA30.7281553398058253No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT30.7281553398058253Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30.7281553398058253Clontech Universal Primer Mix Long (96% over 26bp)
ACATATATTTGGGGAAATTTGCTCAGATGCTTTTTACTGATGAGCTGGAT20.48543689320388345No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTG20.48543689320388345Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA20.48543689320388345Illumina Single End PCR Primer 1 (100% over 21bp)
AAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.48543689320388345Clontech Universal Primer Mix Long (96% over 26bp)
CAATATACAGAAATCAGTAGCACTTCTATAACAACAAGTTGTCTGAAAAG20.48543689320388345No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA20.48543689320388345Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA20.48543689320388345Clontech Universal Primer Mix Long (95% over 22bp)
TGCTATCTCACCAGCTCCTCACCCCCATGTACTCTGCGTTGATACCACTG20.48543689320388345Clontech SMART CDS Primer II A (95% over 23bp)
CATCTCCTCCATCCAGATCCCAAAACATCTTGTTTTTGGACTCATCGTTC20.48543689320388345No Hit
GCTTGAGCCCAGGAGTTCAAGCCCAGCCTTGGAACTGTCTCTTATACACA20.48543689320388345No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA20.48543689320388345Clontech Universal Primer Mix Long (96% over 26bp)
TCCGTGGCCTGACCAATGACGTTTACTTCCAGGTAGGCCTGCTCACAACC20.48543689320388345No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.48543689320388345Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTA20.48543689320388345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT20.48543689320388345Clontech Universal Primer Mix Long (96% over 26bp)
CTGGTTTAGTGGGGGATGGGGTGCTGTCTTTTCCAAGCAGGGGTGTGGCT20.48543689320388345No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA20.48543689320388345Clontech SMART CDS Primer II A (96% over 26bp)
GAAGAACAGAAGTACCCATGGCAGATGACTGAAGAAGCACTGAAAGTGTA20.48543689320388345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.48543689320388345No Hit
ATCCTGACTCACTGCAACCTCTGCTTTCCAGGCTCAAGTGATTCTCGTGC20.48543689320388345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.48543689320388345No Hit
CCCTCTCCCCTACTGGGGCCCATTTCCTGGTCTCTTTCCCTTCTCATCTG20.48543689320388345No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACT20.48543689320388345No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.48543689320388345Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGA20.48543689320388345Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATC20.48543689320388345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAG20.48543689320388345No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCCAGAGT20.48543689320388345Clontech Universal Primer Mix Long (95% over 23bp)
GTACATGGGTGACGCACACGAGATTACACTCTTTCCTACACGGAGCTGCC20.48543689320388345No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20.48543689320388345Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGTGGCGACGGCAGCGAGGCAAAAAAAAAA20.48543689320388345No Hit
CCCTCCCTGAGAGAGGCAACCTTGTGAGAGATGGCATCCTTGTCTGGTGC20.48543689320388345No Hit
GTCCAAAAAAGCACTTGCAGGCTTTACGAACAGAGTGTTTCCAAACTGCT20.48543689320388345No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGAT20.48543689320388345Clontech Universal Primer Mix Long (96% over 28bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC20.48543689320388345Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGACCCATCTAACTCTTCCCTACACGACGCTCTGTCCC20.48543689320388345No Hit
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATA20.48543689320388345Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.48543689320388345No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.48543689320388345Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.48543689320388345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT20.48543689320388345Clontech Universal Primer Mix Long (96% over 26bp)
GCTGAAACATAATTTTTCTCTTTTTGGTTTCACATTTTTACTAAAGACAA20.48543689320388345No Hit
ACCTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCGAAGAATCTG10.24271844660194172Illumina Single End PCR Primer 1 (100% over 30bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAACCAG10.24271844660194172No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGTAAAAAAAAA10.24271844660194172No Hit
TCCCTACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTG10.24271844660194172Illumina Single End PCR Primer 1 (100% over 25bp)
CTATAATATTATGTTAATGGTGGAAGAGTGTAGGAACATACAAAGGAGAT10.24271844660194172No Hit
GTACATGGAAGCAGTGGTATCAACGCAGATACCACTGCTTCCATGTACTC10.24271844660194172Clontech Universal Primer Mix Long (95% over 23bp)
ACGCTCGTTTTTCAGGACCTGTTGGTACCAGACGGAAGAGCGTCGTGTAG10.24271844660194172No Hit
TATCAACGCAGAGTACATGGGAGAACGCGACACCGAGTCTGTCTCTTATA10.24271844660194172No Hit
ACGCAGAGTACATGGGTGACGGCGACCACCGAGATCTACATCTTTCCCTA10.24271844660194172Illumina Single End PCR Primer 1 (95% over 23bp)
TATCAACGCAGAGTACATGGGCGAGTCTACACTCTTTCCCTACACGACGG10.24271844660194172Illumina Single End PCR Primer 1 (96% over 25bp)
ATTTACAACTGTCTGATCTTTGACAAACCTGACAAAAAGAAGAAATGGGG10.24271844660194172No Hit
CTCNTTCCCTACACGACGCTCTTCCGATCTGGTACCAACAGGTCCTGAAA10.24271844660194172Illumina Single End PCR Primer 1 (96% over 30bp)
GAGTACATGGGTAGCCCCGAGTCTACACTCTTTCCTACACGGAGCTGCCG10.24271844660194172No Hit
CTCTTCCGAAGAAAAAAAAAAAAAAAAAAAAAAAAATGAAAAGTGGTTTT10.24271844660194172No Hit
TCCTACTTTCTGTTAGGTTTGGGTTAAATTTGTTCTCATTTTACTAGTTT10.24271844660194172No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGCTGTCTCTTATACAC10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTATAAATATCAAACAGGATGTGGATATTCAAAATCATGCTTTACTTCAG10.24271844660194172No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTC10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAGGCG10.24271844660194172No Hit
CCCTACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGG10.24271844660194172Illumina Single End PCR Primer 1 (100% over 24bp)
GTATCAACGCAGAGTACATGGGTGGCGCCACCGAGATCTACACTCTTTCC10.24271844660194172Illumina Single End PCR Primer 1 (95% over 24bp)
CTGACAGTATTAGACAGATCCTCGAGGCAGAAAACTAACAAAGATATTTG10.24271844660194172No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGCCGTTGATACCACTGCT10.24271844660194172No Hit
TATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAGAGTACATGG10.24271844660194172Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TTTTTGCGTTGATACCTCTGCTTCCCATGTACCTGTCTCTTATACACATC10.24271844660194172No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAAAGTACATGGAAGCAG10.24271844660194172Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACAGAGAGGACAGGGAACTGTTCTGCCTGAAAGTGACTAAAGCCCAGGTC10.24271844660194172No Hit
ACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCCTGTCTCTTA10.24271844660194172Illumina Single End PCR Primer 1 (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATGATACGGC10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGACTGAGATGCTTTGGTTACCTGGTCACAGTATTCTATCCCTGTAGTAG10.24271844660194172No Hit
TTGCTATTGTGAATACGATGCAGCTATAAAAAAGAATGAGATCTTATCCT10.24271844660194172No Hit
GCGCGCATCCCACTGCGCTCACCGCCGCGGGCAAGCGCTTCCAGCCGCTG10.24271844660194172No Hit
TCCCTACACGACGCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGA10.24271844660194172No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGGAGCAACGCAGAGTACAAGG10.24271844660194172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT10.24271844660194172No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.24271844660194172Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT10.24271844660194172No Hit
GAGTACATGGGAACAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.24271844660194172Clontech Universal Primer Mix Long (95% over 22bp)
AGTGAGAGGATTGCTTGAGGCCAGGAGTTTGAGACCAGCATGGGCAACAT10.24271844660194172No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC10.24271844660194172No Hit
GGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAGT10.24271844660194172Clontech Universal Primer Mix Long (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACCCAGAGTACATGGGAAGCAGTGGTA10.24271844660194172Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAA10.24271844660194172No Hit
GATGTGTATAAGAGACAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.24271844660194172No Hit
CCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAGG10.24271844660194172Illumina Single End PCR Primer 1 (100% over 23bp)
GAGTGTAGATGGTGGTCGCCGTATCCCATGTACTCTGCGTTGATACCACT10.24271844660194172Clontech SMART CDS Primer II A (95% over 22bp)
CTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGAGCGTCGTGTAGGG10.24271844660194172Illumina Single End PCR Primer 1 (100% over 24bp)
GCTCTCGTGGCCCGATATCATGGGCCTGATCGTGCTCGAGGGCCTGCTGA10.24271844660194172No Hit
GGTTGTCAGGGGAACCCGGATCACGGGAGCCCAGCGCGAGACGCTCGCCG10.24271844660194172No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTACCGCAACACATT10.24271844660194172No Hit
CGCCGAAGACGATCAAGGTGGTCGCCACGATCCAGAGGTTGCGCAGCGCG10.24271844660194172No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGTCTGTATTATTTCTTACAACTGCATGTGAATCTGTAATTGTCTCAAA10.24271844660194172No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTAT10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGTACGGCGACCACCGAGATT10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTCCAACAAACACTAACATAGTTTCTAATGGCTCAAGGCCACATGCCTAT10.24271844660194172No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGTGAGGCGACACCGATATCTACACTTTTTACCTACACGAC10.24271844660194172No Hit
ATATTAGATTCACATTTATGTGTGTTTTCCTTTGTCTCACTGAAGCAGGC10.24271844660194172No Hit
CCCTACACGACGCTCTTCCGATTTCATGATGCAAGGACTGGAGTTGCTGG10.24271844660194172Illumina Single End PCR Primer 1 (95% over 24bp)
CCACTGCTTCCCATGTACTGTATCAACGCAGAGTACATGGGAAGCAGTGG10.24271844660194172No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)
GTTTCAACGCAGAGTACATGGGCGGAACACTTTCCCTACACGACGCTCGT10.24271844660194172Illumina Single End Sequencing Primer (95% over 22bp)
GTGTAGAAAAGTGTACTGGGCCCCATGTACTCTGCGTTGATACTGTCTCT10.24271844660194172No Hit
GACGGTGGGGACGGACACGGCCTCGGTGATCTCGCGCGCCAGTCCCTCGG10.24271844660194172No Hit
AAAGCACACTACAATGTCAATAAGAGTAATCAACCCAAATTAAATGGAAA10.24271844660194172No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGC10.24271844660194172No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAA10.24271844660194172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTAT10.24271844660194172No Hit
AGACCGGCCCGCGCCAGCTTGGCACCATCGGTTTTGCCACCGTGCGCGGT10.24271844660194172No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAA10.24271844660194172No Hit
TCGTAAGCCAGCCTGGCCAACATGGTGAAACCCTATCTCTACTAAAAAAA10.24271844660194172No Hit
GGTACGGCGACCACCGAGATCAACACACTTTACCAACACCGAGCTGCCCA10.24271844660194172Illumina Single End PCR Primer 1 (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAAGC10.24271844660194172No Hit
CTCCATTCCGGCGCCTGGGCCGGGCGCAGGCCCGTCAGCTGCGGCGCCGC10.24271844660194172No Hit
GTATAAAGTTATATACAGCAGCCACAGGAAACTAATATACCAGGTAAATA10.24271844660194172No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAAGTTAGTCCGGTGGTCCTATCCCC10.24271844660194172Illumina Single End PCR Primer 1 (96% over 26bp)
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAA10.24271844660194172No Hit
CTCCCCAGGAAGAGGGGCCCGGGAAGCTCCACTCTCTGGCAGAAATCCCA10.24271844660194172No Hit
GGCTGTAGAGGGGGCAGCCTGTGCACTTTGCAGCCTGGGCCGCGGTCAGC10.24271844660194172No Hit
CCACTTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACC10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGCTGTCTCTTAT10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)
TCCCTACACGACGTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAGA10.24271844660194172No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAACAATG10.24271844660194172No Hit
GTTTTGAGTGAGTGTCTTAATCCTGAGTTCTAGTTTGATTGCACTGTGGT10.24271844660194172No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCCTGTCT10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)
GAGAAAAATACTGCGGTGAGTGGGAAAGAGGTTGACTGACATTCATATGG10.24271844660194172No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATC10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAACAAAAGA10.24271844660194172No Hit
CCTAAATATTTTCTGCCCCTTTTGGGGACCAAGCAACAAAGTCCAGCCTC10.24271844660194172No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAATACATGGGAA10.24271844660194172Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACGGCTTACCAAGT10.24271844660194172No Hit
GTATCAACGCAGAGTACATGGGAAGCGTGGTATCAACGCAGAGTACATGG10.24271844660194172No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGCGTTTAAACCA10.24271844660194172No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10.24271844660194172Clontech SMART CDS Primer II A (100% over 24bp)
CTCCACCTCCAGGGACAGGATATGGAGCAACAAGGTAAATGGAAACATCC10.24271844660194172No Hit
GAGTTGCTGTAGGAAGAGATCTGGGTCGCCTATCCCATGTACTCTGCGTC10.24271844660194172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAGGCGTTGAAACCAAT10.24271844660194172No Hit
GGTGTAGGCTGTCTCGCCCGAGGGCAGGGTGACCTGGAAATAGCCCTTGC10.24271844660194172No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG10.24271844660194172No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACCAGAGTACATGGGAAGCA10.24271844660194172No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGTGGTCGCCGTATCACCCATGTACTCTGCGTTGATACCAC10.24271844660194172Clontech SMART CDS Primer II A (95% over 21bp)
GTCTCCAGCCCTGTGGCCACCAGGGCTGCTCCTCCCTAGGCCACCCTGCC10.24271844660194172No Hit
ACCTAGCAAGATGAGCCAACATTCAAATTCAGAAAATAAAGAGAACACCT10.24271844660194172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTA10.24271844660194172No Hit
GTTGATACCACTGCTTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.24271844660194172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC10.24271844660194172No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.24271844660194172Clontech SMARTer II A Oligonucleotide (95% over 21bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAACAGCGTTGAT10.24271844660194172No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCAATCTAATCTTCGATTAT10.24271844660194172Clontech SMART CDS Primer II A (100% over 26bp)
CTTACCTAGTTTGCATTTCTTCATCTATTCTTATAATTACTCCTGCCCCA10.24271844660194172No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATG10.24271844660194172No Hit
CACTGGGGTTGATTCCATTCCATTGCATTCCATTCCATTCCATTCCATTC10.24271844660194172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10.24271844660194172No Hit
GTATCAACGCCGAGTACTTTTTTTTTTTTTTGTTTTTTGTTATGTTTTTG10.24271844660194172No Hit
TATGTCACTCTCAGCATTGGACAGATCATCTAGACAGAAAATCAACAAGG10.24271844660194172No Hit
AGACCATAAATCCACAGTTCTTACCCTATGCGGTTTTTCATGAGTAAACT10.24271844660194172No Hit
CTGTAGCCTCGACCTGGTGGGCTCAAGTTATCCTCCCACCTCAGGCCCTC10.24271844660194172No Hit
GTGTAGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGCGGC10.24271844660194172No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCT10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC10.24271844660194172No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAACTGCGTTGATA10.24271844660194172No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGTAC10.24271844660194172Clontech Universal Primer Mix Long (95% over 22bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTATAACAC10.24271844660194172No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.24271844660194172Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTAC10.24271844660194172Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAGATGCGTTTAAACCAC10.24271844660194172No Hit
CATGCCTCAGATTCTGTCGGAAACAGAGATAGGAAGAGCTTCGTTTAGTT10.24271844660194172No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTGGTACCGACAGGTCCTGAAA10.24271844660194172Illumina Single End PCR Primer 1 (100% over 30bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGGGTTTAAAACAC10.24271844660194172No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTC10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGTACGGCGACCACCGGATCTACACTCACGACGCTCTTGC10.24271844660194172No Hit
GTATCAACGCAGAGTACAAGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.24271844660194172Clontech SMART CDS Primer II A (100% over 26bp)
TCACTACAGAGATATGTCACAATGTCCCCTGTAGGCAGAGCATAGGGAAG10.24271844660194172No Hit
TTCCCTGCTGTACCAGTAGACAGTGAGATCGGAAGAGCGTCGTGTAGGGA10.24271844660194172Illumina Single End PCR Primer 1 (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA10.24271844660194172No Hit
CAGCAACTCCAGTCCTTGCATCGATCGGAAGAGCGCGTGTAGGAAGAGGT10.24271844660194172No Hit
GATGAGAGGAGGAGCCAGCGTTGGAGCTCTGGCCTTCCAAGATCCCCATC10.24271844660194172No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGGAAAAAAAAAAAAAAAAAA10.24271844660194172No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCAT10.24271844660194172Clontech Universal Primer Mix Long (95% over 21bp)
GGTCCCGGTCGCGGCGGGGGCACCCCGCCCCCCGCCCGGGGGGGCGGTGG10.24271844660194172No Hit
CGGATAATGCTGCTATGAACATCCTTGCATAAGTTTTTGTGTGGACATAA10.24271844660194172No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAATTTACTCTG10.24271844660194172No Hit
CTCTCAAACTGTCTTTTTCTCATTCCTTTGACTCTGCCAGACTTCATCAC10.24271844660194172No Hit
GTATCAACCGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.24271844660194172No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAATTAC10.24271844660194172Clontech Universal Primer Mix Long (95% over 21bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATAA10.24271844660194172Clontech Universal Primer Mix Long (95% over 22bp)
TATCAACGCAGAGTACATGGGTGTACGGCGACCACGAGACTACACTCATT10.24271844660194172No Hit
GCTTCACCCGTTTCGAGGTGGGCGAGGGCATCGAGAAGGAAGAGGTCGAT10.24271844660194172No Hit
GTACAATAATGGATTCATTCTGATAATTGCTAAGGTGTTTTGAGCCAAAA10.24271844660194172No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.24271844660194172Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTAGGTTTTAAAT10.24271844660194172No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.24271844660194172Clontech SMART CDS Primer II A (100% over 26bp)
GTTATAGAGTTGGAGGGAAATCTGTGTCATTCAATCTTTATGACAAGTAA10.24271844660194172No Hit
GTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACAT10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTACCCATGTACTCTGCGTTGATACCACCTGTCTCTTATACACATC10.24271844660194172Clontech SMART CDS Primer II A (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGGGGTAAAAAAAAAAAAAAAAAAA10.24271844660194172No Hit
AAAGTACTCTGCGTTGATACCGCTGCTTACCCATGTACTCTGCGTTGATA10.24271844660194172Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAAAACA10.24271844660194172No Hit
ACAGCGACTATCGCGGCGAAGTCGGGGTGATCCTCTCCAACATCGGCTCG10.24271844660194172No Hit
GAGACAGAGAGACAAAGAAAGACAGAGACAGAGAGAGGCAAAAAAGACAG10.24271844660194172No Hit
CTTCCGATCTCTGGTACCGACAGAATCTGAGAGCGTCGTGTAGGGAAAGA10.24271844660194172Illumina Single End PCR Primer 1 (95% over 22bp)
GCCAGGAACGGTACTTCTGCTGCCATGATGGTCCAGCTTAAGTACGCGCC10.24271844660194172No Hit
GTTCTGCCCTCGTCTCTACCCAGGGCTCTTGGCTGTGGTGGCTGAGGGAG10.24271844660194172No Hit
CTCCACCTCAGCCTCCTGAGTAGCAGGGACTACAGCCGAGTAGCTGGGAC10.24271844660194172No Hit
CTTATACACATCTGACGCTGCCGACGTCCCATGTACTCTGCGTTGATACT10.24271844660194172No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGATACCACTGCTTCCCATGT10.24271844660194172Clontech Universal Primer Mix Long (95% over 24bp)
TATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCTACAC10.24271844660194172Illumina Single End PCR Primer 1 (100% over 29bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTCATTCACCAGGATTAATGTTCAAAGAGCAACAGAATTCCGCAGCT10.24271844660194172No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCT10.24271844660194172No Hit
TTCTACGGGCAAACCTTCGCCCCATAAAATGACCGGATCCCCGGCTTTGT10.24271844660194172No Hit
GAGTACATGGGAAGCAGTGGTGTCAACGCAGAGTACATGGGAAGCAGTGG10.24271844660194172Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)
CGATTGCAATCGAATGGAATGGAATGGAATTAACCCGAATAGAATGGAAT10.24271844660194172No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACGCAGAGTACATGG10.24271844660194172No Hit
GCAGAGTACATGGGACTTTGGGAGGCCAAGGCGGGTGGATCACCTGAGGT10.24271844660194172No Hit
AACTATAACACTCATACCAGCTAAAAAATCCTTTGTACTTAAAACTCTAA10.24271844660194172No Hit
AGTCTTGAGTCATGTGACTGTAGTCACGGCCCTGCACCTCGGCGACTTGC10.24271844660194172No Hit
AATTTAACCTTGATATAGGCTTCGTATCTAATATACAGTCCACATTCAGA10.24271844660194172No Hit
GCTAAAAATTCACCTTATTGCCTTGTAACAGTTCTCTTGTATGTTATTTG10.24271844660194172No Hit
AAGCCAGCGTCGGGGCTCGCGGCGTGTTGGCTCTGGCGTTGCTCGCCCTG10.24271844660194172No Hit
GTACATGGGAAGCGGGACCGAGACTACACTCTTCCTACTGTCTCTTATAC10.24271844660194172No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTAAATAAAAAAATAAAATAAAAAG10.24271844660194172No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCAGA10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGT10.24271844660194172No Hit
GTATCAACGCAGAGTACATGGGTATGTACGCGACCCGAGATTACACTCTT10.24271844660194172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTAT10.24271844660194172No Hit
ACATCTGACGCTGCGACGAAAAAAAAAGTGTAGATCCGGTGGTCGCCGTA10.24271844660194172No Hit
GCCCCGCAACTCCAGTCCTTCATCATGAGATCGGAAGAGCGTCGTGTAGG10.24271844660194172Illumina Single End PCR Primer 1 (100% over 23bp)
CCCCTCAACCAATACCTTAACTAATTTTACAATTTGGCGAATATTCCTTT10.24271844660194172No Hit
ATATATCACTTCACACCTACAATGATGACTAACATTTTAAAAAGGCAGAT10.24271844660194172No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTCCCGCAGCACCTT10.24271844660194172No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTATTTAT10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAAAAAACC10.24271844660194172No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGCTGTCTCTTATACACATCTG10.24271844660194172No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA10.24271844660194172No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.24271844660194172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGAGACACCGTCTCAAAAAAAAAAAAAAAAAGTACTCTGCGGTATCAAC10.24271844660194172No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCGAAAAAAAA10.24271844660194172No Hit
GAGTACATGGGAATAGGCACCACCGACTTTCCCTACACGACGCTCTTCCG10.24271844660194172Illumina Single End PCR Primer 1 (96% over 25bp)
ACTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTAACACAGGATACCAATT10.24271844660194172No Hit
ATTCCAGTTTGGCCCATTAAAAACCTTCCAAAAGTGATTCTCTGTTCTCT10.24271844660194172No Hit
GAAACCAGATGTCCTTGATATTTTTTGATTTAGCATGAAGACAATGTTCC10.24271844660194172No Hit
ACGCAGAGTACATGGGAAGCAGTCGTATCAACGCAGAGTACATGGGAAGC10.24271844660194172Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGGCGGAGAGGTGGTTGCTCGCCAGGATCGCCGCCCCTTGGTCCGGGAC10.24271844660194172No Hit
GTGTAATCTCGGTCGCCGACATCCCATGTACTCTGCGTTGATACCACTGC10.24271844660194172Clontech SMART CDS Primer II A (95% over 24bp)
GGCTGAGGCGGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAG10.24271844660194172No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATATGCGTTTAA10.24271844660194172No Hit
GGAAGGAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.24271844660194172Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGATGGTCTTGTCTCAGTGTTGATAGCTACTGACTGATCAGGGTGATGGT10.24271844660194172No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATACACATCT10.24271844660194172No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAA10.24271844660194172No Hit
CATGGGATGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAATAAA10.24271844660194172No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAATGCGTTTTAACCAAT10.24271844660194172No Hit
GTATCAACGCAGAGTACATGGGAAGAGTGGTATCTACGCAGAGTACATGG10.24271844660194172No Hit
CCCAGCAACAGCCCGGCGATAGTGACGCCGATACTCATCGACAATTGCAT10.24271844660194172No Hit
ACTTCAGGTCGAGCGCATCTTCGGTTCCATGCAGGCCGGCCATCTGCCCG10.24271844660194172No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAA10.24271844660194172Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)