FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4433
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.6767426122264832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.3383713061132416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG120.27069704489059326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.22558087074216107No Hit
GTACATGGGACTGGCTGCTCTGAAAAGCCATCTTTGCATTGTTCCTCATC80.18046469659372885No Hit
GTCCTGTAGGATCCTCTCCTGCCAGCGTTGGGGCTCCCCGTGGAGTGACC80.18046469659372885No Hit
TTTCGGAGCTGGTGTCTACGGCTGCGTCTGACATGGTGGGGCACGCCGGT80.18046469659372885No Hit
GTACATGGGAGAGCTCGGCGGCTGGGACTGGAGGACAGCGGTGGCGGAGG70.15790660951951274No Hit
GTACATGGGACTCTGGGCAGGATCCAACGTCGCTCCAGCTGCTCTTGACG60.13534852244529663No Hit
CTTCTGGCTGACAGCACAAGAGAAACTCCTAGTTTAGACAACTGGTAAGC60.13534852244529663No Hit
GTACATGGGAGAGCGCGCGAGCCCGGGGCGGGCGGGGCGCGTCCCGGAGG60.13534852244529663No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.13534852244529663No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13534852244529663Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA50.11279043537108054No Hit
TCCCTGTACGCTGCTTCACTTGTGGCAAGATCGTCGGCAACAAGTGGGAG50.11279043537108054No Hit
GTATCAACGCAGAGTACATGGGACTGGCTGCTCTGAAAAGCCATCTTTGC50.11279043537108054No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA50.11279043537108054No Hit
TTTTTGAAGAGATGGGGTCCCACTTGGTCATCCAGGCCAAAGTGCAGTAG50.11279043537108054No Hit
TTTTAGTAGAGACGGGGTTTCACCGTGTTGGCCACAATGGTCTCGATCTC50.11279043537108054No Hit
CTTCCTGATGTTGCCATGGCATTTGTAAACTGTGATGACGCTGGTGGGAG50.11279043537108054No Hit
GTGCTAGATGGCGCCTGGAAGGGGGGTCACCCAAGTCTCCCTGCTGTCAT50.11279043537108054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT50.11279043537108054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)