FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length140
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAGATCGGGCAGGATCCCGGCCCCACCTGCCGGATCTCTGGGTGCA27.142857142857142No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA27.142857142857142No Hit
GCGTGATCACGCCCAGGTAGAACGCGATCAGCCCAAGCGCGGCCAGGAGG27.142857142857142No Hit
ATCTACGACGATCTGCAACTGATGATCAAGCTCGACGCCGACTACGCCGC13.571428571428571No Hit
AGGTTGACGAACATGCCGAAACTCCGGTCACCACAAGAGTCGCCGTAGCA13.571428571428571No Hit
GTCCGCGCTGCGGCAGGAACTGTCGGACTGGATCGACAGCAAGACCGACT13.571428571428571No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT13.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
CCTTCATCTCAAAACACACTTTGCCTTTTGACACACCATAGGATGCTCTT13.571428571428571No Hit
GATGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACACC13.571428571428571No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATAGGCG13.571428571428571No Hit
GTGTGTGAACCCTGCCCTCCAGGCACCTACATTGCCCACCTCAATGGCCT13.571428571428571No Hit
GTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTG13.571428571428571No Hit
GGCTATCTGGGTTGGGCAGCCGAAAGGGGGCTTGAAACCCTGCTGCCCTC13.571428571428571No Hit
GACGATCCCCGCGAGCGCCGCCAGCATCCCCATGTTGACGAAGGTCAGGA13.571428571428571No Hit
GCCCAAGCCCGAGTGCTCTGAGAGCTACACGCTGACGCCGGAGGTGGGGG13.571428571428571No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT13.571428571428571No Hit
ACGATCCTTCTTGCGATCATGGCGTTCGGCCTGTCGCTGGTCGGCACGTT13.571428571428571No Hit
GATCCTTGTCCAGCTCATCCAGATCCCGTTTTATTGACAGGACATCCAAA13.571428571428571No Hit
CTGTCGAACAGGGTGATCTGGCGCTCGTCGGTGCGGCCGGGGGCCTGCCC13.571428571428571No Hit
AAGTGACCGTGGGCGTGTTCACCACCGTGGTGCCGCCCGCGCCAGCCGTC13.571428571428571No Hit
GAGGTACTCCGCGCGGGTGCCCAGACCGTCCTTCTGCACGGTGTCGAGGA13.571428571428571No Hit
GGCCGGCCGCCACTACTCGGCCACCTCCGCAGCGCAGAAGATCTCGCGCG13.571428571428571No Hit
GTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATT13.571428571428571No Hit
GTTAGGGGCGGACTGACACCTCACACGGCTGGGTACTCCTCTGAGACAAA13.571428571428571No Hit
GTCAACGTGCGCTTCAGCACCATCGTGCGGCGCCGGGCCAAGGGCGCCAT13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)