FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGAGCAGGGAGGGGACCAGAACCATATACTGCGGTTTTCCAGAGAC46.557377049180328No Hit
TCCCACGGCCTCTAGGAAGATTCAGTCATGTGCACAGCCAGCTGGCAGAA34.918032786885246No Hit
CCAGAAAAAGTGTTTATTCATCAAGTCTTTAAAGATACAAAAACACGTGT23.278688524590164No Hit
GCATTGTTATCATTAACCAGTCTTTTACTACTAAACTTAAATGGCCAATT23.278688524590164No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23.278688524590164Clontech Universal Primer Mix Long (96% over 26bp)
CCAGGTGGTCCCGCCACGCACCGTCCACGTGGATTGCGGAGCGCCGGAGA11.639344262295082No Hit
CTCCCAAACATGCACACACACACCCCTCTTGTTTTGGGGGAGACAATGAC11.639344262295082No Hit
GAAAGCTCTTCGAACGGCCTCGATGTCGATAGATATTCGGCCTTGGCCCA11.639344262295082No Hit
ATCCAAACCTCTCTCTCCACAGGTGGCTCAAAATCAATCTGAAGAACTGA11.639344262295082No Hit
TGCTGCAGGGTGCTTTCCGCAAACCGCGACAGGTGATGCTTCCAGAAGAG11.639344262295082No Hit
GGACCTACAGCTCTGTTTACTCTATCAAAGACAAAAGTTTCCAGAAACAC11.639344262295082No Hit
ATGTAAGTGTCCATAAACAGACAAATGAATAAAGAAACATGGTTTATGTA11.639344262295082No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTACTCCATGA11.639344262295082No Hit
GTATGGGGATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGG11.639344262295082No Hit
CCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTG11.639344262295082No Hit
GCAATACGTAGACGGGCAGTCTTGCCGAACAAGTGGCCAGCGGAACGCCG11.639344262295082No Hit
CGCTGCTTGCGCCCTACGTTGTAAAAATTACAAAAATGTGGCGCAAGCTA11.639344262295082No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA11.639344262295082Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAGTACTCAGT11.639344262295082Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTAGACGGAGTAA11.639344262295082No Hit
GAGTGGAGCAGGGAGGCGACCAGAACCAAATACTGCGCTATTCCAGAGAC11.639344262295082No Hit
CTCCCGAGCAGCTGGGAGTACAGGCGCCCGCCACCATGCCCGATTAATTT11.639344262295082No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.639344262295082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11.639344262295082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCTGGTCTACGTGCGCATCTACCAGATCGCCAAGCGTCGCACCCGCGT11.639344262295082No Hit
GTCTTCACGAAGATCTGCATACCACCTCTCAGACGCAGGACCAGGTGCAG11.639344262295082No Hit
ACCCGGACGGCACCGAGATCACCAAGGTGGCGACGATGGAGGGGCGGATC11.639344262295082No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC11.639344262295082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCTA11.639344262295082No Hit
GTACATGGGAAGCAGTGGTATCAACGCATTTTTTTTTTTTTTTTTTTTTT11.639344262295082No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
CGGCAAATTGCCCGGTTCGCAGGCCTGTTCGGGCCTGCGAGCAGAGAAAC11.639344262295082No Hit
GGTACTGTGAGGGATAGACAAGCCTCCATCCACAGCTCCCTTCAGGGCAC11.639344262295082No Hit
GTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGA11.639344262295082No Hit
GTAGTGGCGGGCGCCTGCAGTCCCAGCCACTTGGGAGGCTGAGGCAGGAG11.639344262295082No Hit
AGGTAAAAGTTGCAATGGGCTGAGATTGTGCCACTGCACTCCAGCCTGGG11.639344262295082No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC11.639344262295082No Hit
GTGCAGTGGCTCACACCTGTAATCCCAATACTTTGGGAGGCTGAGGTGAG11.639344262295082No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT11.639344262295082No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA11.639344262295082No Hit
AGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCA11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
ACCCAGGACATGTCTCTGTATACCCTATTGGGCAGAATTAATCATAAAGC11.639344262295082No Hit
GCTTCAGTTTCTGCCTCAATAAAAACCAAATGAGATAATAACACAGCAAC11.639344262295082No Hit
ATGCCCTCCAATTGATGACCATCAGGTTCCATACTATCCTAGGTTTGGGG11.639344262295082No Hit
TTGCCGAAGAGGCCAAGCGCGTCTCCGGCGACGTGATGGCCAGCACCTGG11.639344262295082No Hit
GTACTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCCTGGC11.639344262295082Clontech Universal Primer Mix Long (95% over 21bp)
CTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCG11.639344262295082No Hit
GAAGAAACTGCATCAACTAACGAGCAAAATAACCAGCTAACATCATAACG11.639344262295082No Hit
CTGCTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
ACCCCAGGCTGCCGCCGGCGGGCTCGGGCATCCTCGGTCCGGTGGTGCAG11.639344262295082No Hit
GTGTGAGCCACTACCGTGCCAGGCCTGATATAATTAGATTTAAATTAATT11.639344262295082No Hit
TCCCCACGGCGGGCAGCAGCGGCGGCGGCGGCGACTGAACGCGGAGGGGG11.639344262295082No Hit
GCGCAGAGTACATAGATTAAGGGATTTTGCTTCCTTCTGAACTAGATCAT11.639344262295082No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)