FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003643746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003643746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTCACAGTCACATGTCCAGGGACAGGCTTCCCATATGTGTATAGGCCA46.557377049180328No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34.918032786885246Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCAGCTCCAACCTCTTGGGTTCAGGTGATCCTCCCACCTCTGCCTCCT34.918032786885246No Hit
GGTAAAAAGCTGGAAGATGGCCCTAAATTCTTGAAGTCTGGTGATGCTGC23.278688524590164No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
GATCCAGGATAAGGAAGGCATTCCCCCCGACCAGCAGAGGCTCATCTTTG11.639344262295082No Hit
GGTAATACAGAAGAGAAATTAATTAATATAGTATCATTAAAAAAAAAAAA11.639344262295082No Hit
CCCGTATAGAGGGGGATATGATAGTCTGCGCAGCGTATGCACACGAACTG11.639344262295082No Hit
GTTCATTTTTTTTCCCTTTTAGTTAAGCATGACTTTAGATGGGAAGCCTG11.639344262295082No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGAGTACTTTTTTTTTTTTTTG11.639344262295082Clontech SMART CDS Primer II A (95% over 23bp)
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGT11.639344262295082No Hit
CTCCTGATTTCTATCTCCACAAACTTCACTTCTTGTCATGAATTTACTGC11.639344262295082No Hit
GGAACAGGCCGCGTTGATTCTCGTCCCGAGGGACGCGCATCGGTAGGACG11.639344262295082No Hit
GTTGGAGACATTGTGAATTTCAGATTTGTATTAACCACTTTTTTTGCTAT11.639344262295082No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAGTACCTGTC11.639344262295082Clontech Universal Primer Mix Long (95% over 22bp)
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC11.639344262295082No Hit
GAAATAAGCCAAGCTCAGAAAGACAAATGGCTAATGGATACAAGGATACA11.639344262295082No Hit
CCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGC11.639344262295082No Hit
ATTGTACTCAGCAATTGCAAACTCATCCGTCTCGTAGTGTCTGATTACTA11.639344262295082No Hit
CATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCA11.639344262295082No Hit
CGCCCTGCATGGCCGGACGTTCGTGGATGAGCTCTTCTGGAAGCATCACC11.639344262295082No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
GTGTTGGGCTACTGGATCGATCAGCGCCTGGCCGGGCGCGGCATCGTGCC11.639344262295082No Hit
ACCACACAGACACATGTCCAGGGACAGGCTTCCCATAAGTGTTTACGCCA11.639344262295082No Hit
GTAATCCTAGCACTTTGGGAGGCCGAAGCGGGCAGATCACGAGGTCAGGA11.639344262295082No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.639344262295082No Hit
CTATTAGTTCTTTTTTACGGTGGAACTCAGCAGTGAAGCCATCAGGTCTT11.639344262295082No Hit
AATTCACCCCTTACATTTTTCTGCAAAAGAAAATTAACACTTATCTTCTG11.639344262295082No Hit
CCACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACGAGTTTTCACC11.639344262295082No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
CGGCTTCACCTGGCTCGCTCGGGCCTTGCCTTTGCCCCGGGGACCGCGCT11.639344262295082No Hit
GACCCATCCAGCTACCCCGGGCCACCCTGCAGTGGAAAGGACAGTGTCAG11.639344262295082No Hit
CTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG11.639344262295082No Hit
ACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTT11.639344262295082No Hit
ATTGTACTCAGCAATTGCAATCTCATCCGTCTCGTAGTGTCTGATTACTA11.639344262295082No Hit
GGGTTACATGTCACGGGCGGCATTCCTCATGGATAAAGTCATGAGCCGTG11.639344262295082No Hit
CACCACAGCTCTAAGGAAGACTTCAAGTCCCAAAGCTGGAGACAGGGACC11.639344262295082No Hit
TTTTTTTTTTTTTTTTTCGTTGATACCACAGCTTCCCATGTACTCTGCGT11.639344262295082No Hit
GACTAGGATTGCAACCCCTGCCTTTTTATGTTTTCCATTTGCTTGGTAGA11.639344262295082No Hit
GGCCTGCCCGGCACCGCCGGGATCGGCGGCACCGTGCCTTCCTGCTTCCA11.639344262295082No Hit
GATTGAACTTCCGCGAGTCAATCTTGGTACCTCGATACCGTCACAGGACT11.639344262295082No Hit
GATCAAAGACCATCTGGTGACCGAAGACGCTGACCGTCCCCGTTCCGGTA11.639344262295082No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT11.639344262295082No Hit
TTATTTCATCTTGTAAAAACATGTGAAGACAACGTTCGTTCTGTGCCAGA11.639344262295082No Hit
GCGCGGAACAGCACTTCCATGCTCTTGGAAAGGATGACGCCCTCGGTGAA11.639344262295082No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA11.639344262295082No Hit
CAACAGCGCCTCCTCCCTTCACTCATTCTTCAGGAATCCTCCGCATCTCT11.639344262295082No Hit
TCTTTTGCTGGAGTCCACGGGAAAAAGATACTAGAAGCTAGGATGCCCTG11.639344262295082No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG11.639344262295082Clontech Universal Primer Mix Long (96% over 26bp)
GATGAACGGCGCATGGCCGCCCAGTTCAAGCGCCACCTTCTTCACCGTCG11.639344262295082No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)